T-cell receptor variable β genes show differential expression in CD4 and CD8 T cells

Large granular lymphocyte expansions in patients with Felty's syndrome: analysis using anti-T cell receptor Vβ-specific monoclonal antibodies

“…Since this study was commenced it has become apparent ihat there are differences between TCR V3 usage by CD4 and CD8 cells [27,28]. Infections involving bacterial superantigens, however, can lead to alterations of both the CD4 and CD8 repertoires.…”

T-cell clones derived by CD3 stimulation from hepatitis C virus explanted liver tissue are not representative of dominant clones present in vivo

“…21 Previous studies using reverse transcription polymerase chain reaction (rtPCR) to assess TCR expression by intrahepatic T cells in chronic HCV infection were performed using unsorted T-cell populations as the starting material. 3,22 Although differences in TCR use were observed, it is difficult to interpret these data because TCR use varies solely as a function of T-cell subset, 12 and clonal expansions are known to occur in the CD8 T-cell compartment in healthy individuals. [23][24][25] In the work reported here, CD8 T cells were excluded from the analysis by negative selection with anti-CD8 mAb before rtPCR and by positive selection with anti-CD4 mAb at the T-cell cloning step.…”

“…4 Our previous studies have shown that this technique results in less than 1% CD8 T cells remaining in the sample. 12 This population of cells contains the resident CD4 T cells referred to throughout this report. The term resident is intended to stress that the CD4 T cells in these samples reflect the actual population of T cells present in end-stage livers before in vitro culture.…”

“…Two T-cell clones from patient 1 were identified twice (clones 1-17 and 5-11) in the cells sorted with anti-BV2. The nomenclature used to refer to each clone (for example, [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] indicates that clones 1 and 17, arbitrarily assigned these numbers when cultured in microtiter plates, turned out to be identical or sibs of each other on sequence analysis. One T-cell clone from patient 3 was also isolated twice (clone 3-7).…”

“…First-strand complementary DNA (cDNA) was prepared from 0.5 to 3.0 ϫ 10 6 of the population of cells containing resident CD4 T cells with random hexanucleotides, 12 and spectratyping was performed as previously described. 4 The sequences of the TCRBV2, 3, 5S1, and TCRBC primers have previously been reported.…”