T-cell receptor variable region gene usage in T-cell populations.

Garman, Richard D.; Ko, Jone-Long; Vulpe, Christopher D.; Raulet, David H.

doi:10.1073/pnas.83.11.3987

Cited by 53 publications

(27 citation statements)

References 45 publications

(37 reference statements)

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“…In concert with the analysis of the population, these data reveal a situation in which most adult thymic y/S cells express one y chain, although a minor set of cells expressing other y chains is present in the population. This situation is in contrast to that of the Va and VD chains of the a/P TCR, where predominant V gene use is not generally observed (35,36). It seems unlikely that the predominant usage of Vy2 by thymic y/S cells is due to preferential rearrangement of the Vy2 gene in early T cells (as has been argued for the predominant usage ofJH-proximal VH gene segments in pre-B cells [37] because fetal thymocytes preferentially rearrange the Jy l-proximal Vy3 and Vy4 genes (10) .…”

Section: Discussionmentioning

confidence: 44%

Predominant variable region gene usage by gamma/delta T cell receptor-bearing cells in the adult thymus.

Korman

Marusić-Galesić

Spencer

et al. 1988

The Journal of Experimental Medicine

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Most T lymphocytes express a clonally distributed cell surface antigen receptor composed of disulfide-linked a and (3 subunits, noncovalently associated with several subunits called CD3 proteins . T cells that express the a/(3 form of the T cell receptor (TCR) recognize antigens in the context of cell surface proteins encoded by class I and class II genes of the major histocompatibility complex (MHC). A small distinct subset of peripheral T lymphocytes (

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Section: Discussionmentioning

confidence: 44%

Predominant variable region gene usage by gamma/delta T cell receptor-bearing cells in the adult thymus.

Korman

Marusić-Galesić

Spencer

et al. 1988

The Journal of Experimental Medicine

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“…This is, to our knowledge, the first structural analysis of rearranged TCR genes among human T cell clones in specific responses. Skewed V segment use among alloreactive T cells has also been observed in the mouse (25,(37)(38)(39), but very few V gene sequences were reported in these studies (25).…”

Section: Resultsmentioning

confidence: 99%

T cell receptor V beta gene usage in a human alloreactive response. Shared structural features among HLA-B27-specific T cell clones.

Bragado

Lauzurica

López

et al. 1990

The Journal of Experimental Medicine

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A strategy, based on using V beta family-specific oligonucleotides, was developed for specific amplification and direct sequencing of human TCR V beta genes. With this strategy, it was possible to undertake a structural analysis of TCRs from human T cell clones in specific responses. 12 HLA-B27-specific cytotoxic clones were examined. The results reveal a nonrandom use of V beta gene diversity in this alloreactive response in that: (a) the clones express a restricted number of V beta segments, including a subset of V beta families that are significantly more related to one another than to most other V beta families; (b) five of seven clones having a particular reaction pattern with HLA-B27 subtypes possess Alanine at the D-J junction; and (c) identical J beta segments are found associated in several instances with identical or highly homologous V beta gene segments. In addition, two new V beta 13 members are reported.

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“…The complexity of the a-chain locus has made analysis of this chain particularly difficult, as there are >100 Va and 80 Ja gene segments (8,19). To overcome these problems, we used the RNase protection assay (15) and the PCR (16) and investigated the frequency of a particular a chain mRNA in the thymus and spleen.…”

Section: Results and Discussion Predominant Expression Of The V14+ Tcmentioning

confidence: 99%

Homogenous junctional sequence of the V14+ T-cell antigen receptor alpha chain expanded in unprimed mice.

Koseki

Imai

Nakayama

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

108

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Nucleotide sequences of VJ (variable-joining) junctional regions of V14' a-chain T-cell receptor genes show that most VaW4+ T cells use one a chain (Val4Ja281 with a one-nucleotide N region, which is frequently used in keyhole limpet hemocyanin-specific suppressor T-celi hybridomas) in unprimed mice. Moreover, the frequency of this a-chain expression was >1.5% of the total a chains found in laboratory strains, including B10 congenic mice. This is about 104 times higher than was expected. The V14J281 a-chain expression was relatively low but was significant in CD41/CD81 immature thymocytes and became quite high in mature single-positive T cells, implying that this a chain is selected during T-cell maturation. V14J281 expression increased with time after birth and reached a maximum at around 5 weeks of age. The ligand seems to be a self molecule and to be present in laboratory strains but to be absent in a wild mouse, Mus musculus molossinus, because bone marrow chimeras clearly showed that bone marrow cells derived from Mus musculus molossinus negative for this a chain raised V14J281-positive T cells in a C57BL/6 environment. The above results suggest that there are some selection mechanisms for this cell type other than those for conventional afi T cells and also that the homogenous VJ junction of the V14J281 a chain plays a pivotal role in the selection of the T cell and its ligand reactivity.Thymus-derived lymphocytes (T cells) recognize antigens in the context of the polymorphic parts of major histocompatibility complex (MHC) class I or class II molecules by virtue ofthe heterodimeric a/3 T-cell receptor (TCR), which is found on the vast majority of mature T cells in the thymus and peripheral lymphoid tissues (1, 2). A second TCR, y8 TCR, has also been identified (3, 4) and found to be relatively abundant in some adult mouse organs and among fetal thymocytes.The T-cell repertoire seems to be generated by two selection mechanisms during T-cell We provide evidence herein that some T cells use a homogenous a chain (V14J281). This seems to be a consequence of selection and expansion ofthis T-cell type, because its frequency is >1.5% of the total a chains in the peripheral lymphoid organs. Moreover, studies using bone marrow chimeras suggest that the ligand is an unidentified self molecule. The results indicate that the homogenous VJjunctional region of the V14J281 a chain, in particular, plays a crucial role in the selection of this type of T cell and its ligand reactivity. MATERIALS AND METHODSAnimals and Cell Lines. Pathogen-free C57BL/6 mice were purchased from Shizuoka Experimental Animal Co., Hamamatsu, Japan. A wild Japanese mouse strain, Mus musculus molossinus, was established and maintained by K.M. Other strains, including B10 congenic lines used, were also maintained by K.M. A thymoma cell line of AKR origin, BW5147, and a keyhole limpet hemocyanin (KLH)-specific suppressor T-cell (Ts) hybridoma (BW5147 x C57BL/6 Ts; 34S-281) used in the present studies have been described (12,13

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T-cell receptor variable region gene usage in T-cell populations.

Cited by 53 publications

References 45 publications

Predominant variable region gene usage by gamma/delta T cell receptor-bearing cells in the adult thymus.

Predominant variable region gene usage by gamma/delta T cell receptor-bearing cells in the adult thymus.

T cell receptor V beta gene usage in a human alloreactive response. Shared structural features among HLA-B27-specific T cell clones.

Homogenous junctional sequence of the V14+ T-cell antigen receptor alpha chain expanded in unprimed mice.

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