T cell receptor repertoire of infiltrating T cells in lips of Sjögren's syndrome patients.

Sumida, Takayuki; Yonaha, F; Maeda, Toshiro; Tanabe, E; Koike, Takao; Tomioka, Hisao; Yoshida, Sho

doi:10.1172/jci115635

Cited by 140 publications

(101 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tissue from the lips of 3 SS patients (patients 8, 10, and 12) with predominant expression of V{52 and Vf313 genes was used for sequencing experiments (4). As shown in Figure 1, a total of 41 cDNA clones encoding the V{52 D-J-C gene were sequenced.…”

Section: Resultsmentioning

confidence: 99%

“…To elucidate the nature of these infiltrating T cells, we analyzed the T cell receptor (TCR) repertoire using the quantitative polymerase chain reaction (PCR). We found that the repertoire of the TCR V{3 gene on infiltrating T cells from the lips of SS patients was not restricted; however, V{32 and V{313 genes were predominantly expressed in the lips of these SS patients (4).…”

mentioning

confidence: 81%

See 1 more Smart Citation

Restricted junctional usage of T cell receptor Vβ2 and Vβ13 genes, which are overrepresented on infiltrating T cells in the lips of patients with sjögren's syndrome

Yonaha

Sumida

Maeda

et al. 1992

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. To analyze the clonality of T cell receptor (TCR) Vβ2—and Vβ13—positive T cells, which are predominantly expressed in the lips of patients with Sjögren's syndrome (SS).Methods. The junctional sequences of complementary DNA clones encoding TCR Vβ2 and Vβ13 genes were determined by the polymerase chain reaction. Forty‐one Vβ2 and 45 Vβ13 clones established from the lips of 3 SS patients were sequenced.Results. The Vβ2/Jβ2.3 pair was enriched in 2 of the 3 patients (44% and 46% of the clones, respectively), and the Vβ13/Jβ2.1 sequence was dominant in 2 of the 3 (23% and 45%). These pairs were not used preferentially in peripheral blood lymphocytes from the same patients.Conclusion. Infiltrating Vβ2‐ and Vβ13‐positive T cells from the lips of all 3 patients with SS were polyclonal, but the junctional usage of cells from 2 lip samples was restricted, compared with cells from peripheral blood. This suggests that not all expanded cells from the lips of SS patients are stimulated by superantigens.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 81%

Restricted junctional usage of T cell receptor Vβ2 and Vβ13 genes, which are overrepresented on infiltrating T cells in the lips of patients with sjögren's syndrome

Yonaha

Sumida

Maeda

et al. 1992

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…We have reported that the repertoire of T cell receptor V, gene on the infiltrating T cells from the labial salivary glands (LSGs) of SS patients is not restricted, but the V,2 and V,13 genes are predominantly expressed in the LSGs of these patients (2). Furthermore, these Vp2-and VP13-positive T cells expand polyclonally , but the junctional usage is relatively limited and an amino acid at position 106 in complementarity-determining region 3 (CDR3) is conserved (3).…”

mentioning

confidence: 99%

Expression of sequences homologous to htlv‐i tax gene in the labial salivary glands of japanese patients with sjögren's syndrome

Sumida

Yonaha

Maeda

et al. 1994

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. To address the question of whether the human T cell leukemia virus type I (HTLV‐I) gene is associated with the etiology of Sjögren's syndrome (SS). Methods. RNA expression of HTLV‐I gag, pol, env, and tax genes in labial salivary glands (LSGs) from SS patients who were seronegative for antibodies to HTLV‐I was examined using reverse transcription and polymerase chain reaction techniques. Results. The HTLV‐I tax gene, but not the HTLV‐I gag, pol, or env genes, was detected in LSG samples from 4 of 14 patients (29%). The nucleotide sequences of the HTLV‐I pXIV region in these 4 patients' LSGs showed 100% homology to the HTLV‐I pXIV gene from the MT‐2 cell line. Conclusion. These findings suggest that products encoding sequences homologous to the HTLV‐I pXIV gene in SS patients' LSGs might be candidates for self‐antigen and/or lead to activation of autoreactive T lymphocytes through trans‐acting transcriptional activation.

show abstract

“…Twenty different V, and C, primers or 5'-C, and 3'-C, primers (as a control) were used for PCR. The sequences of V and C, primers were from previously published data (7). dligonucleotides were synthesized in a DNA synthesizer (Applied Biosystems, Foster City, CA).…”

Section: Methodsmentioning

confidence: 99%

T cell receptor vβ repertoire of double‐negative α/β t cells in patients with systemic sclerosis

Sakamoto

Sumida²,

Maeda³

et al. 1992

Arthritis & Rheumatism

View full text Add to dashboard Cite

T cell receptor repertoire of infiltrating T cells in lips of Sjögren's syndrome patients.

Cited by 140 publications

References 21 publications

Restricted junctional usage of T cell receptor Vβ2 and Vβ13 genes, which are overrepresented on infiltrating T cells in the lips of patients with sjögren's syndrome

Restricted junctional usage of T cell receptor Vβ2 and Vβ13 genes, which are overrepresented on infiltrating T cells in the lips of patients with sjögren's syndrome

Expression of sequences homologous to htlv‐i tax gene in the labial salivary glands of japanese patients with sjögren's syndrome

T cell receptor vβ repertoire of double‐negative α/β t cells in patients with systemic sclerosis

Contact Info

Product

Resources

About