T-cell receptor alpha-chain gene is split in a human T-cell leukemia cell line with a t(11;14)(p15;q11).

Abstract

Chromosomal rearrangements in malignant T-cell disease frequently involve the chromosome bands containing the T-cell receptor genes. The RPMI 8402 cell line, which was established from the leukemia cells of a patient with T-cell acute lymphoblastic leukemia, is characterized by a translocation involving chromosome 14 (band q11) and chromosome 11 (band p15) [t(11;14)(p15;q11)]. By using in situ chromosomal hybridization and Southern blot analysis to examine RPMI 8402 cells, we determined that the break at 14q11 occurs within the variable region sequences of the T-cell receptor alpha-chain gene (TCRA); the break at 11p15 occurs between the HRAS1 gene and the genes for insulin and the insulin-like growth factor 2. These results suggest that the TCRA sequences activate a cellular gene located at 11p15 in malignant T-cell disorders.

The t(11;14)(p15;q11) in a T-cell acute lymphoblastic leukemia cell line activates multiple transcripts, including Ttg-1, a gene encoding a potential zinc finger protein.

“…The der (11) firmed by hybridization to flow-sorted chromosomes from 8402 but could also be inferred from the known chromosomal orientation of the a/8TCR locus (19,34). However, the flow-sorted chromosomes provided an important reagent for localizing other genes telomeric or centromeric to these breakpoints at 14qll and lipiS.…”

Physical mapping of 3 candidate tumor suppressor genes relative to Beckwith-Wiedemann syndrome associated chromosomal breakpoints at 11p15.3

“…RBTN1 was previously mapped with a hybrid panel, proximal to the J 1-10 hybrid breakpoint at 11 p i 5.3 (Henry et al, 1993). In contrast, Le Beau et al (1986) mapped the T-ALL breakpoint itself between IGF2 and HRAS. In this study we confirm the mapping of RBTN 1 proximal to the Jl-10 hybrid breakpoint.…”

Molecular Biology and Genetic Advances in Childhood Malignancies

“…A significant series of these translocations have now been characterized (Table 4.3). In the first group, which is characteristic of the lymphoid malignancies, an oncogene is translocated into a chromosomal region associated with the immunoglobulin genes or T-cell receptor genes (BISHOP 1989;HALUSKA et al 1987;KAGAN et al 1989;VON LIND ERN et al 1990;LE BEAU et al 1986). In the first group, which is characteristic of the lymphoid malignancies, an oncogene is translocated into a chromosomal region associated with the immunoglobulin genes or T-cell receptor genes (BISHOP 1989;HALUSKA et al 1987;KAGAN et al 1989;VON LIND ERN et al 1990;LE BEAU et al 1986).…”