T Cell Memory to Vaccination

Todryk, Stephen

doi:10.3390/vaccines6040084

Cited by 26 publications

(25 citation statements)

References 24 publications

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“…Subunit vaccines often suffer from poor immunogenicity and require certain helper molecules known as adjuvants to induce or enhance an appropriate immune response to the antigen. T‐cell activation is crucial in inducing protective immune responses (Todryk, 2018). Antigens mannosylated by P. pastoris have shown to have enhanced antigen presentation and T‐cell activation properties compared with their nonglycosylated counterparts.…”

Section: The Characteristic Features Of P Pastoris Expression Systemmentioning

confidence: 99%

Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

Keikha

Rezaee

Farsiani

2020

Journal Cellular Physiology

384

220

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One of the most important branches of genetic engineering is the expression of recombinant proteins using biological expression systems. Nowadays, different expression systems are used for the production of recombinant proteins including bacteria, yeasts, molds, mammals, plants, and insects. Yeast expression systems such as Saccharomyces cerevisiae (S. cerevisiae) and Pichia pastoris (P. pastoris) are more popular. P. pastoris expression system is one of the most popular and standard tools for the production of recombinant protein in molecular biology. Overall, the benefits of protein production by P. pastoris system include appropriate folding (in the endoplasmic reticulum) and secretion (by Kex2 as signal peptidase) of recombinant proteins to the external environment of the cell. Moreover, in the P. pastoris expression system due to its limited production of endogenous secretory proteins, the purification of recombinant protein is easy. It is also considered a unique host for the expression of subunit vaccines which could significantly affect the growing market of medical biotechnology. Although P. pastoris expression systems are impressive and easy to use with well-defined process protocols, some degree of process optimization is required to achieve maximum production of the target proteins. Methanol and sorbitol concentration, Mut forms, temperature and incubation time have to be adjusted to obtain optimal conditions, which might vary among different strains and externally expressed protein. Eventually, optimal conditions for the production of a recombinant protein in P. pastoris expression system differ according to the target protein.

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Section: The Characteristic Features Of P Pastoris Expression Systemmentioning

confidence: 99%

Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

Keikha

Rezaee

Farsiani

2020

Journal Cellular Physiology

384

220

View full text Add to dashboard Cite

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“…Upon exposure to that pathogen at some time in the future, long‐lived memory cells awaken and recognize the antigen against which they were generated and mount a pathogen‐specific response, resulting in protection against the infection and associated disease. [ 15,16 ]…”

Section: Vaccination and Immunological Memorymentioning

confidence: 99%

“…A subset of these T and B cells, in turn, differentiates into long‐lived memory cells that are able to respond almost immediately upon re‐exposure to that antigen, bypassing the need for a de novo response. [ 15–20 ] It is, therefore, necessary for vaccine formulations to be designed with different combinations of antigens to promote protective immunity through strong initial interactions with APCs.…”

Section: Vaccination and Immunological Memorymentioning

confidence: 99%

Nonthermal plasma as part of a novel strategy for vaccination

Mohamed

Esposito

Kutzler

et al. 2020

Plasma Processes & Polymers

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Vaccination has been one of the most effective health intervention mechanisms to reduce morbidity and mortality associated with infectious diseases. Vaccines stimulate the body's protective immune responses through controlled exposure to modified versions of pathogens that establish immunological memory. However, only a few diseases have effective vaccines. The biological effects of nonthermal plasma on cells suggest that plasma could play an important role in improving efficacy of existing vaccines and overcoming some of the limitations and challenges with current vaccination strategies. This review summarizes the opportunities for nonthermal plasma for immunization and therapeutic purposes.

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“…For the evaluation of immune response, we performed four immunizations (on days 1, 14, 28, and 42). This scheme had an extra booster compared to the scheme described below, which was used in order to enable the generation of a supposed "protective" level of effector T cells [31]. This model ended on day 43, when the animals were euthanized to obtain the genitouterine tract, blood, spleen, and small intestine.…”

Section: Oral Immunization and Gbs Vaginal Colonizationmentioning

confidence: 99%

Mucosal Vaccination with Lactococcus lactis-Secreting Surface Immunological Protein Induces Humoral and Cellular Immune Protection against Group B Streptococcus in a Murine Model

et al. 2020

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Group B Streptococcus (GBS) is the primary etiological agent of sepsis and meningitis in newborns and is associated with premature birth and stillbirth. The development of a licensed vaccine is one of the pending challenges for the World Health Organization. Previously, we showed that oral immunization with surface immune protein (SIP) decreases vaginal colonization of GBS and generates functional opsonizing antibodies, which was determined by opsonophagocytic assays (OPA) in vitro. We also showed that the protein has an adjuvant vaccine profile. Therefore, an oral vaccine based on SIP may be an attractive alternative to employ in the development of new vaccines against GBS. Lactococcus lactis is a highlighted oral vaccine probiotic inducer of the mucosal immune response. This bacterium could serve as an antigen-delivering vehicle for the development of an edible vaccine and has been used in clinical trials. In this study, we showed that an oral vaccine with a recombinant L. lactis strain secreting SIP from GBS (rL. lactis-SIP) can induce protective humoral and cellular immunity in an experimental model of GBS vaginal colonization in C57BL/6 mice. Mice immunized with rL. lactis-SIP were protected against clinical symptoms and bacterial colonization after GBS vaginal colonization. Our rL. lactis-SIP vaccine also induces an increase of immunoglobulin G (IgG) and immunoglobulin A (IgA) specifically against SIP. The adoptive transfer of serum from vaccinated mice to naïve mice generated protection against GBS vaginal colonization. Moreover, the rL. lactis-SIP strain induces the activation of SIP-specific T cells, which could decrease GBS vaginal colonization and generate protective antibodies when transferred to other mice. Our experimental observations strongly support the notion that rL. lactis-SIP induces protective humoral and cellular immunity and could be considered as a novel alternative in the development of vaccines for GBS.

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T Cell Memory to Vaccination

Cited by 26 publications

References 24 publications

Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

Nonthermal plasma as part of a novel strategy for vaccination

Mucosal Vaccination with Lactococcus lactis-Secreting Surface Immunological Protein Induces Humoral and Cellular Immune Protection against Group B Streptococcus in a Murine Model

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