T‐Cell‐Dependent Production of IgG by Human Cord Blood B Cells in Reconstituted SCID Mice

Abstract

Reconstitution of severe combined immunodeficient (SCID) mice with human lymphocytes has recently allowed the elucidation of abnormalities of immune responses in various immunological disorders. In the present study, mononuclear cells (MNC) from neonatal cord blood and adult peripheral blood were intraperitoneally injected into SCID mice to examine induction of human Ig in respective mice recipients. Human IgG was consistently detected in the serum of SCID transferred with adult MNC, but only a few SCID recipients of cord blood MNC showed detectable but low levels of IgG in the serum. The combination experiments of isolated B and T cells disclosed that some interactions between B and T cells might be necessary for IgG production in transferred SCID mice. Notably, transfer of cord blood B cells with adult but not cord blood T cells resulted in efficient induction of IgG, associated with a change in subclass distribution. The results suggest that inability of neonatal B cells to produce IgG can be overcome by transfer with adult mature T cells into SCID mice.

Control of human thyroid autoantibody production in SCID mice

“…Representative experiments are depicted to illustrate the Our results confirm previous findings that successful reconkinetics seen in the experiments. Figure 2 illustrates the IgG, Tg stitution of SCID mice, based on IgG levels, is dependent on the antibody and TPO antibody produced over time in experiment presence of T cells [11][12][13]. Our study extends these findings to T8 and TI 1.…”

“…Overall, Tg antibody was detected in 7/14 mice CD4+ and CD8+ cell requirements in the production of Tg and reconstituted with control PBMC, 0/6 mice given T-enriched TPO antibodies in recipient SCID mice PBMC and 0/14 mice given T-depleted PBMC. TPO antibody SCID mice were reconstituted with PBMC, CD4-depleted was detected in 7/11 mice reconstituted with control PBMC, 1/6 PBMC and CD8-depleted PBMC from AITD patients and mice given T-enriched PBMC and 2/11 mice given T-depleted plasma samples were taken over the following [12][13][14][15][16][17] weeks. PBMC.…”

Dexamethasone Inhibits Maturation and Alters Function of Monocyte-Derived Dendritic Cells from Cord Blood

“…In addition, glucocorticoids are used postnatally to reduce lung inflammation and to improve cardiovascular status in critically ill infants (4). Dx-treated infants have alterations in immune responsiveness as indicated by significantly reduced antibody responses after immunization (5); increased infections (6); multiple short-term adverse complications such as intestinal perforation (7-9), hypertension, and hypertrophic cardiomyopathy (10,11); and long-term effect such as increased risk for airway hyperresponsiveness and decreased pulmonary function (12,13).Several in vitro studies that were designed to identify the stage of maturation of each immune cell lineage in normal newborns have revealed that neonate naïve T (14 -16) and B (17,18) …”

“…Several in vitro studies that were designed to identify the stage of maturation of each immune cell lineage in normal newborns have revealed that neonate naïve T (14 -16) and B (17,18) cells are as competent as their adult counterparts. However, neonatal dendritic cells (DCs) are functionally less competent (19) and less capable of secreting cytokines that are essential for development of Th1 responses and balanced immunity (20).…”

“…Several in vitro studies that were designed to identify the stage of maturation of each immune cell lineage in normal newborns have revealed that neonate naïve T (14 -16) and B (17,18) …”