T-cell-dependent B-cell stimulation is H-2 restricted and antigen dependent only at the resting B-cell level.

Andersson, Jan; Schreier, Max H.; Melchers, Fritz

doi:10.1073/pnas.77.3.1612

Cited by 173 publications

(67 citation statements)

References 23 publications

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“…However, because of the significant differences in the activation properties of resting B cells compared with nonresting B cells (5)(6)(7)(8)(9), this question can be resolved only by using a uniform source of resting B cells. To this end, we have taken advantage of a unique antigen (sheep erythrocyte, SRBC)-specific B-cell lymphoma, CH12 (10), that has the activation properties of a resting B cell (11).…”

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confidence: 99%

“…The cell-dependent activation of CH12 can be achieved in the presence of H-2' (I-Ek)-restricted SRBC-specific Th cells and the specific antigen, SRBC. The requirement for specific antigen and H-2-restricted Th cells for activation, as displayed by CH12 (11), is a property of resting B cells (5)(6)(7)(8)(9). Like resting B cells (5,6) tiation to antibody secretion is an end stage in the development of CH12 and that the inability to induce a larger fraction of cells to secrete is related to their proliferative phenotype.…”

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confidence: 99%

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Role of cell surface immunoglobulin in B-lymphocyte activation.

Locascio¹,

Haughton²,

Arnold³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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The role of cell surface immunoglobulin in helper T-cell-dependent B-cell activation was analyzed using a B-cell lymphoma, CH12, with known antigen specificity and activation properties similar to those of a resting B cell. Two sources of helper T cells were used, both selected such that they interact with H-2-encoded determinants on CH12 in the absence of the specific B-cell antigen, sheep erythrocytes. By this dissociation of the specificity of the T cells from that of the B cells, the requirement for antigen in the induction of CH12 to antibody secretion could be studied. The results show that both helper T-cell-B-cell interactions and surface immunoglobulin-antigen binding are involved in inducing B-cell differentiation, thus establishing a signalling function for the antigen receptor on B lymphocytes. Our data also show that the requirement for surface immunoglobulin-ligand interactions in B-cell activation can, under certain conditions, be circumvented, notably when high (nonphysiologic) multiplicities of Tcell help are used.The binding of antigen to surface immunoglobulin (sIg) on the B-cell membrane facilitates the interaction of antigenspecific helper T (Th) cells with specific B cells by "bridging" the two cell types. Whether the function of the sIg molecule is to passively focus T-cell help onto the B-cell membrane (1) or whether the binding of antigen to sIg also fulfills a signalling function (2) has been a matter of contention. Resolution of this controversy requires that the Th-cell and Bcell specificities for antigen be dissociated, and T-cell specificity must be selected such that efficient T-B interaction occurs independently of the involvement of sIg. Such a method is suggested by the work of Augustin and Coutinho (3) MATERIALS AND METHODS Animals. B10.H-2aH-4bp/Wts (2a4b) mice were bred and maintained under pathogen-free conditions at the University of North Carolina. (BALB/c x C57BL/6)F1 (CB6F1, H2d/b), BALB/c (C, H-2d), and C3H/HeJ (C3H, H-2) mice were purchased from The Jackson Laboratory and maintained in the animal colony at Duke University. All mice were 8-to 12-wk old when used. CH12 Lymphoma Cells. CH12 arose in a 2a4b mouse and was propagated as an ascites tumor. The induction, characterization, and identification of the tumor cells' specificity for SRBC have been described (10). From the ascites, 94.3 + 5.5% of the cells were ,u by immunofluorescence (10 experiments) while 1.2 ± 0.4% were nonspecific esterase positive (four determinations). The role, if any, that these latter cells in the tumor preparation might play in the activation of CH12 has not been analyzed. In 8 experiments, 89.9 ± 6.4% of the cells rosetted with SRBC but none with human erythrocytes or mouse erythrocytes (MRBC). We have recently found that this apparent specificity for SRBC is likely to be a cross-reaction with autologous effete erythrocytes, because CH12 rosettes as efficiently with bromelain-treated MRBC (12) as with SRBC. Greater than 95% of cells are positive for the CH12 idiotype, as determined...

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mentioning

confidence: 99%

mentioning

confidence: 99%

Role of cell surface immunoglobulin in B-lymphocyte activation.

Locascio¹,

Haughton²,

Arnold³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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“…The role of a direct major histocompatibility complex (MHC)-restricted interaction between thymus-derived helper cells (TH) and B cells in the latter's activation to immunoglobulin secretion has been widely investigated (1)(2)(3)(4)(5)(6)(7)(8). Recently, it has been established that the requirement for a MHC-restricted TH-B interaction correlates with the B cell's state of activation: the induction of resting (small) B cells is limited by this interaction whereas the induction/amplification of activated (blasted) B cells is not (7,9).…”

mentioning

confidence: 99%

Dissociation of two signals required for activation of resting B cells.

Julius¹,

Boehmer²,

Sidman³

1982

Proc. Natl. Acad. Sci. U.S.A.

114

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“…Thus, antigen-specifically produced "T cell help" appeared to activate B cells antigenunspecifically. Finally, we found that helper T celldependent B cell stimulation was MHC-restricted and antigen-dependent only at the resting, G 0 -level of a B cell [34].…”

Section: B Cell Stimulation With the Help Of T Cellsmentioning

confidence: 86%

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Melchers

2007

Eur. J. Immunol.

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T-cell-dependent B-cell stimulation is H-2 restricted and antigen dependent only at the resting B-cell level.

Cited by 173 publications

References 23 publications

Role of cell surface immunoglobulin in B-lymphocyte activation.

Role of cell surface immunoglobulin in B-lymphocyte activation.

Dissociation of two signals required for activation of resting B cells.

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