T-cell clones and T-cell receptors.

Fitch, F W

doi:10.1128/mmbr.50.1.50-69.1986

Cited by 28 publications

(6 citation statements)

References 185 publications

(309 reference statements)

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“…The addition of exogenous T-cell factors restored the response of L3T4-depleted but not Lyt-2depleted spleen cells. This finding is in accordance with the notion that the main function of CD4 T cells in the generation of alloreactive cytolytic T-cell responses is the secretion of lymphokines, particularly IL-2, which influence the replication and differentiation of CD8 cytolytic effector T cells (3,6). Recently, however, evidence for the autonomous generation of Lyt-2+ cytolytic T cells has been obtained in some systems as well (23).…”

Section: Discussionsupporting

confidence: 88%

“…In contrast, transfer of Lyt-l+ T cells had no effect. (The Lyt-1 marker is preferentially expressed by L3T4+ T cells; however, Lyt-2+ T cells also bear the Lyt-1 molecule, although at a lower density [6,16].) While these data suggest that Lyt-2+ T cells are involved in the expression of protection against tuberculosis, they do not exclude a role for L3T4 + T cells in the acquisition of anti-tuberculous resistance.…”

Section: Discussionmentioning

confidence: 93%

“…In general, CD4 T cells with helper functions express the L3T4 marker in mice (T4 in humans) and are class II restricted (for summaries, see references 3, 6, and 26). These features distinguish them from cytolytic CD8 T cells which commonly are Lyt-2+ (mice), T8+ (humans), and class I restricted (3,6,26). Cytolytic T cells are generally thought to be primarily responsible for anti-viral imtnunity.…”

mentioning

confidence: 99%

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Impaired resistance to Mycobacterium tuberculosis infection after selective in vivo depletion of L3T4+ and Lyt-2+ T cells

Müller¹,

Cobbold²,

Waldmann³

et al. 1987

Infect Immun

253

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The resistance of mice against Mycobacterium tuberculosis infection after selective in vivo depletion of L3T4+ and Lyt-2+ T cells was studied. Thymectomized mice were treated with rat monoclonal antibodies against the L3T4 or Lyt-2 molecule to selectively eliminate the respective T-cell subset. In both L3T4+ and Lyt-2+ T-cell-depleted mice, resistance against subsequent infection with M. tuberculosis was markedly impaired compared with that in untreated controls, with L3T4+ T-cell-depleted mice showing more pronounced effects. Simultaneous depletion of L3T4+ and Lyt-2+ T cells did not further exacerbate infection. These findings suggest that both L3T4+ and Lyt-2+ T cells are involved in the acquisition of resistance against tuberculosis.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 93%

mentioning

confidence: 99%

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Impaired resistance to Mycobacterium tuberculosis infection after selective in vivo depletion of L3T4+ and Lyt-2+ T cells

Müller¹,

Cobbold²,

Waldmann³

et al. 1987

Infect Immun

253

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“…It has become increasingly clear that CD4 (T4, L3T4) and CD8 (T8, Lyt-2) T cells react with different major histocompatibility complex-encoded molecules: antigen recognition by CD4 T cells is class I1 restricted, whereas CD8 T cells are class I restricted. Furthermore, evidence is accumulating that the CD4 (T4, L3T4) and CD8 (T8, Lyt-2) molecules are involved in the interaction of the corresponding T cells with their target cells [6]. Initially, it was thought that this dichotomy is also associated by a strict functional dissociation with CD4 (T4, L3T4) T cells delivering helperhnducer signals and CD8 (T8, Lyt-2) T cells being responsible for target cell lysis.…”

Section: Discussionmentioning

confidence: 99%

“…It is generally assumed that macrophage ( M a ) activation by T cell-derived lymphokines represents the central step in the development of acquired resistance against listeriosis as well as other intracellular bacterial infections [l]. Adoptive transfer studies in the murine listeriosis model have provided good evidence that both class 11-restricted L3T4' and class I-restricted Lyt-2' T lymphocytes are required for effective antibacterial protection [2-51. Although exceptions exist, class 11-restricted CD4 T cells (L3T4' in mice, T4' in man) preferentially express helpedinducer functions whereas class I-restricted CD8 T cells (Lyt-2' in mice, T8' in man) predominantly exert cytolytic activity (for review see [6]). According to this scheme the major task of CD4 T cells in acquired antibacterial resistance should be the secretion of lymphokines which activate antimicrobial functions in infected MQ,.…”

Section: Introductionmentioning

confidence: 99%

Specific lysis of Listeria monocytogenes‐infected macrophages by class II‐restricted L3T4⁺ T cells

et al. 1987

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Mice were infected with the intracellular bacterium, Listeria monocytogenes, and T cell clones from spleens, lymph nodes and peritoneal exudates were established. The capacity of L3T4+, Lyt2- T-cell clones to specifically lyse L. monocytogenes-infected macrophages was analyzed. As a source of target cells, bone marrow macrophages (BMM phi) after 9 days of culture in hydrophobic teflon bags were used. These BMM phi were totally Ia-; however, significant Ia-expression could be induced by interferon-gamma (IFN-gamma). IFN-gamma-stimulated BMM phi, after priming with live or killed L. monocytogenes organisms were effectively lysed by the vast majority of L3T4+ T cell clones. In the absence of either IFN-gamma stimulation or antigen priming, no lysis occurred. Cytolysis was demonstrable in a conventional 4-h 51Cr-release assay and in an 18-h neutral red uptake assay and was antigen specific and class II restricted. Native T cells from L. monocytogenes-infected mice failed to lyse stimulated, L. monocytogenes-primed BMM phi and gained their cytolytic activity after antigenic restimulation in vitro. These data demonstrate that L. monocytogenes-specific L3T4+ T cells could lyse M phi presenting listerial antigens provided that Ia antigen expression had been induced. L3T4+ T cell clones produced IFN-phi after restimulation with antigen plus accessory cells in vitro and IFN-gamma secretion could be increased by costimulation with recombinant IL 2. These T cell clones conferred significant protection upon recipient mice which was more pronounced in the liver. The possible relevance of lysis by L3T4+ T cells of infected M phi to protection against and pathogenesis of intracellular bacterial infections is discussed.

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CD4 monoclonal antibody pairs for immunosuppression and tolerance induction

et al. 1987

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A pair of rat anti-mouse CD4 monoclonal antibodies (mAb) have been selected which bind to different epitopes of the molecule. Both the mAb are rat IgG2b and show clear synergistic activity in complement lysis in vitro. When injected together in vivo, they exhibit an improved immunosuppressive effect, compared to each antibody alone, on allogeneic graft rejection, humoral responses and on tolerance induction. Limiting dilution analysis indicates that the in vivo depletion of interleukin 2-producing cells is improved using both mAb by 2-3-fold over that obtained with the individual antibodies. As little as 60 ng per mouse of the CD4 antibody pair was sufficient to allow the induction of tolerance to human gamma-globulin, even without elimination of the CD4+ cells. The results suggest that appropriate antibody pairs may be good candidates for effective immunosuppressive serotherapy in man.

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T-cell clones and T-cell receptors.

Cited by 28 publications

References 185 publications

Impaired resistance to Mycobacterium tuberculosis infection after selective in vivo depletion of L3T4+ and Lyt-2+ T cells

Impaired resistance to Mycobacterium tuberculosis infection after selective in vivo depletion of L3T4+ and Lyt-2+ T cells

Specific lysis of Listeria monocytogenes‐infected macrophages by class II‐restricted L3T4⁺ T cells

CD4 monoclonal antibody pairs for immunosuppression and tolerance induction

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T-cell clones and T-cell receptors.

Cited by 28 publications

References 185 publications

Impaired resistance to Mycobacterium tuberculosis infection after selective in vivo depletion of L3T4+ and Lyt-2+ T cells

Impaired resistance to Mycobacterium tuberculosis infection after selective in vivo depletion of L3T4+ and Lyt-2+ T cells

Specific lysis of Listeria monocytogenes‐infected macrophages by class II‐restricted L3T4+ T cells

CD4 monoclonal antibody pairs for immunosuppression and tolerance induction

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Product

Resources

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Specific lysis of Listeria monocytogenes‐infected macrophages by class II‐restricted L3T4⁺ T cells