T Cell and Mononuclear Phagocyte Populations of the Human Small and Large Intestine

Trejdosiewicz, Ludwik K.; Malizia, Giuseppe; Badr-Eldin, Shaimaa M.; Smart, C J; Oakes, David; Southgate, Jennifer; Howdle, P D; Jánossy, G.; Poulter, L. W.; Losowsky, M. S.

doi:10.1007/978-1-4684-5344-7_54

Cited by 20 publications

(18 citation statements)

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“…4). This distribution where APC are localized in T cell areas is similar to that of analogous cells found in other species [19,24,25,28,33]. VegaLopez et al [32], Rothkotter et al [23] and Bianci et al [3], reported low numbers of CD2 + cells in pig lamina propria (LP) at birth, and that these numbers increased significantly from 7 weeks after birth.…”

Section: Discussionsupporting

confidence: 75%

Ontogeny of Pig Discrete Peyer's Patches: Expression of Surface Antigens.

Makala

Kamada

Nagasawa

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. Leukocyte populations present in the discrete Peyer's patches (PP) of the pig were characterized from birth (Day 0) to day 35 after birth by immunohistochemistry and image analysis. Immediately after birth, cell membrane expression of CD2 and CD3, major histocompatibilty complex (MHC) class II (both SLA (swine leukocyte antigen) -DQ + and SLA-DR + ), CD21, 74-22-15 and surface immunoglobulin (sIg) were all demonstrable. Computer assisted morphometric techniques were used to confirm the significant expansion of these cell populations from birth onwards. The distribution of the cell types was not random but suggested a preferential retention of cells at specific sites. This implies a degree of organization of immunological cells within the discrete PP, enhancing the potential to mount immune responses in the most efficient manner. KEY WORDS: discrete Peyer's patch, ontogeny, surface antigen expression, swine.J. Vet. Med. Sci. 63(6): 625-636, 2001 The development of peripheral lymphoid organs has been investigated morphologically by many authors [6, 8,26,29,30]. Most of these studies dealt with the lymphoid cells: B cells, T cells and plasma cells [2,10]. Other reports have been focused on the ontogeny of the non-lymphoid cells in peripheral lymphoid organs: macrophages, interdigitating cells, follicular dendritic cells, and reticulum cells [9,29,30]. Internally, lymphoid organs comprise several compartments [7], each with their own function [13] and characteristic cell population [31]. Within the Peyer's patches (PP), the cooperation between diverse types of lymphoid and nonlymphoid cells is essential for optimal immune response. The aim of this work was to provide information regarding interactions of lymphoid and non-lymphoid cells and to investigate the development of these cells in the pig discrete PP, the major organized lymphoid structures involved in the induction of mucosal immune responses in the intestine. MATERIALS AND METHODSAnimals: Samples were taken from newborn Large White/ Landrace hybrid piglets. All piglets were maintained in conventional conditions on a commercial diet (Willets, Bristol, UK). They ranged from 0-35 days of age and both sexes were used. Piglets were sacrificed at 0 (presuckled), 1, 2, 4, 6, 8, 10, 12, 14, 18, 21, 28 and 35 days after birth. From day 0 to day 12, 3 piglets, 1 from each litter, were used, and from day 14 to day 35, 1 piglet from 1 litter was used. The piglets during this period (day 0-day 35) were not weaned. Tissue collection and preparation: Tissue blocks approximately 5-15 mm × 10 mm, were excised and snap frozen in OCT cryoprotection medium (Tissue-Tek, London, UK) using liquid nitrogen-cooled isopentane. Tissues were labeled for identification by writing on the back of the corkmounting disk. The tissues were wrapped in aluminium foil and polybags to prevent dehydration and stored at -70°C until further processing.Peroxidase anti-peroxidase staining: Serial 4-6 µm frozen sections were cut onto multi-spot, poly-l-lysine precoated slides, dried for 1...

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Section: Discussionsupporting

confidence: 75%

Ontogeny of Pig Discrete Peyer's Patches: Expression of Surface Antigens.

Makala

Kamada

Nagasawa

et al. 2001

The Journal of Veterinary Medical Science

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“…In the epithelium of the normal jejunum, a "blast marker" (CD7) is expressed mainly on the T8 cells, indicating that they are stimulated (Trejdosiewicz et al 1987), which is in line with morphological features (Marsh 1975). However, most IEL seem to be negative for typical activation markers such as major histocompatibility complex (MHC) class II and Tac antigens.…”

Section: Nature Of Intraepithelial Lymphocytesmentioning

confidence: 90%

“…However, most IEL seem to be negative for typical activation markers such as major histocompatibility complex (MHC) class II and Tac antigens. In both the jejunum and colon the IEL are also mainly negative for the H366 antigen of cytotoxic cells, suggesting that most of the intraepithelial T8 cells are functional suppressor (Ts) cells (Trejdosiewicz et al 1987). As the IEL are often found along the basement membrane, apparently crossing it in either direction, they usually seem to leave the epithelium quite rapidly by reentering the lamina propria (Marsh 1975).…”

Section: Nature Of Intraepithelial Lymphocytesmentioning

confidence: 98%

Overview of the Mucosal Immune System

Brandtzæg

1989

Current Topics in Microbiology and Immunology

179

102

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“…2). 2 22 In the epithelium of the normal jejunum, a 'blast marker' (CD7) is expressed mainly on the T8 cells, indicating that they are stimulated,23 24 which is in line with morphological features.25 Most intraepithelial lymphocytes, however, seem to be negative for other activation markers such as major histocompatibility complex class II and Tac antigens.192026 Both in the jejunum and colon the intraepithelial lymphocytes are also mainly negative for the H366 antigen of cytotoxic cells, indicating that the intraepithelial T8 cells are functional suppressor cells.24 As intraepithelial lymphocytes are usually found along the basement membrane, apparently crossing it in either direction, most of them probably leave the epithelium quite rapidly by re-entering the lamina propria.27 It is possible that they do to some extent perform their immunoregulatory function(s) in the latter microenvironment. Suppressor and helper activities have been indicated from in vitro studies of human intestinal intraepithelial lymphocytes in cocultures with peripheral blood mononuclear cells, apparently depending on the Tto B-cell ratios.2829 It seems likely that some of the immunoregulatory effects observed with crude cell preparations were caused by interferon-y or other lymphokines produced by stimulated intraepithelial lymphocytes (Fig.…”

Section: Distribution and Particular Features Of Mucosal Lymphocytesmentioning

confidence: 99%