T Cell Activation in Pediatric AIDS Pathogenesis: Three-Color Immunophenotyping

Plaeger-Marshall, Susan; Isacescu, Valentin; O’Rourke, Sheryl; Bertolli, Jeanne; Bryson, Yvonne J.; Stiehm, E. Richard

doi:10.1006/clin.1994.1046

Cited by 66 publications

(51 citation statements)

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“…Immune activation in HIV infection may differ in adults and children inasmuch as most children are infected perinatally and HIV is introduced into a naive and developing immune system (9). Information regarding immune activation in HIV-infected adults can therefore not be extrapolated to the pediatric population.…”

Section: Discussionmentioning

confidence: 99%

“…Newborns express high levels of CD38 on their T cells that decrease with age (2,8,11). CD38 in this patient population is therefore a marker of immaturity as well as of the immune activation that occurs in HIV infection (9).…”

Section: %Cd8mentioning

confidence: 91%

“…In the pediatric setting, the situation appears unresolved. In infants and older children, increased CD8 ϩ T cells expressing CD38 (%CD8 ϩ CD38 ϩ ) have been documented to be positive predictors of survival in HIV infection (4 -6) but have also been associated with advancing disease (7)(8)(9).…”

Section: %Cd8mentioning

confidence: 99%

“…CD38 is ubiquitously expressed on lymphocytes in a unimodal distribution (9,12). Therefore, in HIV-uninfected or -infected patients, cells that express CD38 are difficult to clearly distinguish from those that do not ( Fig.…”

Section: %Cd8mentioning

confidence: 99%

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CD38 Expression on CD8+ T Cells as a Prognostic Marker in Vertically HIV-Infected Pediatric Patients

Sherman

2002

Pediatric Research

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Section: Discussionmentioning

confidence: 99%

Section: %Cd8mentioning

confidence: 91%

Section: %Cd8mentioning

confidence: 99%

Section: %Cd8mentioning

confidence: 99%

See 2 more Smart Citations

CD38 Expression on CD8+ T Cells as a Prognostic Marker in Vertically HIV-Infected Pediatric Patients

Sherman

2002

Pediatric Research

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“…This was shown by analyzing the relative CD38 mean flourescence intensity (MIF) of CD8þ T cells, where an increase in values is observed from control (7.4) to good and partial VLRs (14.7 and 15.3, respectively), and with a further increase in poor VLRs (22.9). In HIV-1 infected adults, the high expression of CD38 on CD8þ T cells has been associated with a poorer prognosis and/or lack of response to ART (38)(39)(40)(41)(42)(43)(44).…”

Section: Nk and B Cell Counts And Expression Of Cd5 On B Cells In Thementioning

confidence: 99%

HIV‐1‐infected children on HAART: Immunologic features of three different levels of viral suppression

Zaccarelli-Filho¹,

Ono²,

Machado³

et al. 2006

Cytometry Part B Clinical

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Background: HIV-1-infected children show changes of blood lymphocyte subpopulations. We have, therefore, investigated how highly active anti-retroviral therapy (ART) alter these subsets. Blood samples were taken from 41 HIV-1-infected children on ART who were divided into groups showing good, partial and poor responses to ART on the basis of viral load (VL) measurement in blood. The observations were compared to those seen in 20 uninfected children.Methods: The samples were studied using 4-color flow cytometry for ''naïve'', central memory and effector memory cells as well as for CD38 expression as the sign of activation within both the CD4+ and the CD8+ T cell populations. HIV-1 infected children were also evaluated for the presence and the titers of antibodies induced by vaccination against childhood infections in our patients while on HAART.Results: Lymphocyte counts were lower in the \poor" viral load responding (VLR) group when compared with partial and good VLRs. Poor VLRs had lower total and naïve CD4+ T cell counts. HIV-1-infected children from all three groups had high CD8+ T cell counts. Central memory CD4+ and CD8+ T cell percentages were particularly low in the poor VLR group while in the poor VLR group the percentages of effector memory CD4+ and CD8+ T cells were higher when compared with the control group. Higher cellular activation of CD8+ T cells was observed in HIV-1-infected children, particularly when analyzed for the intensity of CD38 expression in the poor VLR group. CD5 expression on B cells was higher among all HIV-1-infected children. Antibodies to tetanus, diphtheria, measles, rubella, and hepatitis B were present in a large proportion of children but the titers were similarly low for all three groups of HIV-infected children.Conclusions: Children with different levels of viral response to HAART present immune phenotype characteristics that tend to place the children with partial and good virological responses into the same group. These children are still moderately deficient in their immune responses but show better recovery than seen with children in the poor VLR group. These observations indicate that the proportions of central memory cells among the CD4+ T cells and the intensity of the expression of CD38 activation antigen on CD8+ T cells provide more informative parameters for monitoring children on HAART than the absolute numbers of CD4+ and CD8+ T cells alone. q

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Indicators of T-cell activation: Correlation between quantitative CD38 expression and soluble CD8 levels in asymptomatic HIV+ individuals and healthy controls

et al. 1998

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T Cell Activation in Pediatric AIDS Pathogenesis: Three-Color Immunophenotyping

Cited by 66 publications

References 0 publications

CD38 Expression on CD8+ T Cells as a Prognostic Marker in Vertically HIV-Infected Pediatric Patients

CD38 Expression on CD8+ T Cells as a Prognostic Marker in Vertically HIV-Infected Pediatric Patients

HIV‐1‐infected children on HAART: Immunologic features of three different levels of viral suppression

Indicators of T-cell activation: Correlation between quantitative CD38 expression and soluble CD8 levels in asymptomatic HIV+ individuals and healthy controls

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