t-Butyl hydroperoxide-induced changes in the physicochemical properties of human erythrocytes

Corry, W. D.; Meiselman, Herbert J.; Hochstein, Paul

doi:10.1016/0005-2736(80)90101-7

Cited by 55 publications

(16 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While, verapamil did not show such protective effect on the phospholipid peroxidation. In electrophoretic analysis of erythrocyte membrane proteins, several investigators have reported the appearance of high-molecular-weight products without any change in electrophoretic pattern with tBHP treatment [11,15,17]. However, our electrophoretic analysis of 1 mM tBHP-treated erythrocytes showed the degradation of spectrin, band 3, band 4.2, and band 4.5 accompanied by the appearance of new broad bands of low-molecular-weight products, without the appearance of high-molecular-weight products.…”

Section: Discussionmentioning

confidence: 99%

Verapamil Prevents Impairment in Filterability of Human Erythrocytes Exposed to Oxidative Stress

Okamoto

Maruyama

Kaji

et al. 2004

JJP

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Verapamil Prevents Impairment in Filterability of Human Erythrocytes Exposed to Oxidative Stress

Okamoto

Maruyama

Kaji

et al. 2004

JJP

View full text Add to dashboard Cite

“…95% confidence limits 1 in alteration of the ocular lens (Fukui et al 1980;Varma, Kurnar & Richards 1979;, but the effect of long-term lower concentrations of hydrogen peroxide on cornea endothelial aging and degenerative changes is not yet known. It is of interest that the threshold of toxicity of an organic peroxide, t-butyl hydroperoxide, on human erythrocytes is 0.75 m M which is the same order of magnitude found in this experiment for hydrogen peroxide (Corry, Meiselman & Hochstein 1980). Exposure of erythrocytes to a lower concentration of t-butyl hydroperoxide for an extended period of time, however, also resulted in an alteration of red cell properties (Corry, Meiselman & Hochstein 1980).…”

Section: Iron Chelatementioning

confidence: 88%

“…It is of interest that the threshold of toxicity of an organic peroxide, t-butyl hydroperoxide, on human erythrocytes is 0.75 m M which is the same order of magnitude found in this experiment for hydrogen peroxide (Corry, Meiselman & Hochstein 1980). Exposure of erythrocytes to a lower concentration of t-butyl hydroperoxide for an extended period of time, however, also resulted in an alteration of red cell properties (Corry, Meiselman & Hochstein 1980). Work originally done by Haber & Weiss in 1934 and recently modified has shown that the extremely reactive hydroxyl radical can be formed by the interaction of superoxide anion with hydrogen peroxide in the presence of iron chelates and may explain OH.…”

Section: Iron Chelatementioning

confidence: 91%

Hydrogen Peroxide and Corneal Endothelium

Hull

Csukas

Green

et al. 1981

Acta Ophthalmologica

View full text Add to dashboard Cite

Because of recent evidence of low levels of hydrogen peroxide in the aqueous humor, studies were performed to determine levels of corneal endothelial toxicity as well as factors modifying toxicity. Perfusion of cornea endothelial cells for 3 h with varying concentrations of hydrogen peroxide demonstrated a threshold of toxicity at a concentration between 0.3 and 0.5 mM H2O2. The toxic effect resulted in rapid corneal swelling as well as disruption of endothelial cell cytoplasm and organelles. Both the physiologic and anatomic toxic effects of 0.5 mM H2O2 could be blocked with 5400 U/ml catalase. Exposure of corneas to 20 mM H2O2 for 10 min in the presence of EDTA - Fe+3 resulted in an enhancement of corneal swelling rate more rapid than that which resulted from a 10 min exposure to 20 mM H2O2 alone. Neither the presence of ascorbic acid nor the absence of glutathione and adenosine had and effect on the cornea swelling rate which occurred during a 3 h perfusion of endothelium with 0.3 mM H2O2. Chelated iron had no effect on the corneal swelling induced by phototactivation of rose bengal presensitized cornea endothelial cells.

show abstract

“…Experimentally, oxidative haemolysis can be induced in vitro by incubating intact isolated erythrocytes in the presence of synthetic organic peroxides such as tert-butylhydroperoxide, or by their exposure to conditions which favour an enhanced endogenous production of lipid and/or hydrogen peroxide (Corry et al, 1980;Trotta et al, 1981;Thornalley et al, 1983). When the intracellular uptake and/or generation of peroxides surpasses the capacity of the cell to reductively remove these species, part of the peroxide molecules undergo decomposition, via a metal-or a P-450-catalysed reaction, into radical species capable of damaging proteins and polyunsaturated fatty acids present within the erythrocyte membrane.…”

Section: Introductionmentioning

confidence: 99%