T Antigen Expression in Benign Hyperplasia and Adenocarcinoma of the Prostate (With 1 color plate)

Moriyama, Hiroyuki; Nakano, Hiroshi; Igawa, Mikio; Nihira, Hiromi

doi:10.1159/000281868

Cited by 12 publications

(8 citation statements)

References 7 publications

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“…The TF antigen is well established as an oncodevelopmentally regulated, cancer‐associated antigen that is occluded from normal cells and widely distributed among human tumors, especially in colorectal cancers 27, 28. In any of these cases, increased TF occurrence correlates with cancer progression and metastasis 29, 30. The significance of this work is that it represents the first attempt to image TF antigen‐associated colorectal cancer using topically applied fluorescence nanoparticles in human colorectal cancer tissues.…”

Section: Discussionmentioning

confidence: 98%

Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer

Kumagai

Pham

Kataoka

et al. 2012

Intl Journal of Cancer

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This research aimed to validate the specificity of the newly developed nanobeacon for imaging the Thomsen-Friedenreich (TF) antigen, a potential biomarker of colorectal cancer. The imaging agent is comprised of a submicron-sized polystyrene nanosphere encapsulated with a Coumarin 6 dye. The surface of the nanosphere was modified with peanut agglutinin (PNA) and poly(N-vinylacetamide (PNVA) moieties. The former binds to Gal-β(1–3)GalNAc with high affinity while the latter enhances the specificity of PNA for the carbohydrates. The specificity of the nanobeacon was evaluated in human colorectal cancer cells and specimens, and the data was compared with immunohistochemical staining and flow cytometric analysis. Additionally, distribution of the nanobeacon in vivo was assessed using an “ intestinal loop” mouse model. Quantitative analysis of the data indicated that approximately 2 μg of PNA were detected for each mg of the nanobeacon. The nanobeacon specifically reported colorectal tumors by recognizing the tumor-specific antigen through the surface-immobilized PNA. Removal of TF from human colorectal cancer cells and tissues resulted in a loss of fluorescence signal, which suggests the specificity of the probe. Most importantly, the probe was not absorbed systematically in the large intestine upon topical application. As a result, no registered toxicity was associated with the probe. These data demonstrate the potential use of this novel nanobeacon for imaging the TF antigen as a biomarker for the early detection and prediction of the progression of colorectal cancer at the molecular level.

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Section: Discussionmentioning

confidence: 98%

Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer

Kumagai

Pham

Kataoka

et al. 2012

Intl Journal of Cancer

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“…Normal glandular epithelium expresses S-T and S-Tn antigens, but neither Tn nor T antigens (72). Hyperplastic prostate glands express increased amounts of ABH antigens (138), and have been shown to express sialosyl-T, but no T antigens (139).…”

Section: B the Prostatementioning

confidence: 99%

Tissue distribution of histo‐blood group antigens.

Ravn

Dabelsteen

2000

APMIS

266

223

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The introduction of immunohistochemical techniques and monoclonal antibodies to specific carbohydrate epitopes has made it possible to study in detail the tissue distribution of histo-blood group antigens and related carbohydrate structures. The present paper summarizes the available data concerning the histological distribution of histo-blood group antigens and their precursor structures in normal human tissues. Studies performed have concentrated on carbohydrate antigens related to the ABO, Lewis, and TTn blood group systems, i.e. histo-blood group antigens carried by type 1, 2, and 3 chain carrier carbohydrate chains. Histo-blood group antigens are found in most epithelial tissues. Meanwhile, several factors influence the type, the amount, and the histological distribution of histoblood group antigens, i.e. the ABO, Lewis, and saliva-secretor type of the individual, and the cell- and tissue type. Oligosaccharides with blood-group specificity are synthesized by the stepwise action of specific gene-encoded glycosyltransferases. In general, this stepwise synthesis of histo-blood group antigens correlates with cellular differentiation. The H and the Se genes both encode an al-2fucosyltransferase, which is responsible for the synthesis of blood group antigen H from precursor disaccharides. A new model for the participation of the Se/H-gene-encoded glycosyl transferases in synthesis of terminal histo-blood group antigens in human tissues is proposed; the type and degree of differentiation rather than the embryologic origin determines whether it is the H or the Se gene-encoded transferases that influence expression of terminal histo-blood group antigens in tissues.

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“…In cancer and pre-cancerous conditions such as ulcerative colitis, unsubstituted Galβ1-3GalNAc occurs [5] in about 90% of all human cancers [6,7] including colon [5,[8][9][10], breast [11][12][13], bladder [14,15], prostate [16,17], liver [18], ovary [19] and stomach [20,21]. In many of these cases, the increased TF occurrence correlates with cancer progression and metastasis [22,23]. For example, TF expression is four to six times higher in invasive than in non-invasive bladder cancer [15,24].…”

Section: Introductionmentioning

confidence: 99%

The oncofetal Thomsen–Friedenreich carbohydrate antigen in cancer progression

2007

Glycoconj J

164

158

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The oncofetal Thomsen-Friedenreich carbohydrate antigen (Galbeta1-3GalNAcalpha1-Ser/Thr TF or T antigen) is a pan-carcinoma antigen highly expressed by about 90% of all human carcinomas. Its broad expression and high specificity in cancer have attracted many investigations into its potential use in cancer diagnosis and immunotherapy. Over the past few years increasing evidence suggests that the increased TF occurrence in cancer cells may be functionally important in cancer progression by allowing increased interaction/communication of the cells with endogenous carbohydrate-binding proteins (lectins), particularly the members of the galactoside-binding galectin family. This review focuses on the recent progress in understanding of the regulation and functional significance of increased TF occurrence in cancer progression and metastasis.

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T Antigen Expression in Benign Hyperplasia and Adenocarcinoma of the Prostate (With 1 color plate)

Cited by 12 publications

References 7 publications

Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer

Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer

Tissue distribution of histo‐blood group antigens.

The oncofetal Thomsen–Friedenreich carbohydrate antigen in cancer progression

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