T-antigen-dependent transcriptional initiation and its role in the regulation of human neurotropic JC virus late gene expression.

Abstract

The multifunctional protein of papovaviruses, Tantigen, regulates the virus lytic cycle partly by exerting transcriptional control over viral and cellular gene expression. In this study, the ability of the T-antigen of human neurotropic JC virus (JCV) to enhance expression from the virus late promoter has been further examined. By deletion analysis, a T-antigen-responsive region was mapped within the JCV 98 bp enhancer/promoter between nucleotides 139 and 168. Interestingly, T-antigen appears to mediate transactivation by increasing expression from a basal transcriptional initiation site and through a novel T-antigen-dependent initiation site (TADI). The TADI element contains a region hom-

JC Virus T-Antigen DNA in Gastrointestinal Mucosa of Immunosuppressed Patients: A Prospective, Controlled Study

“…Structurally, JCV contains only six genes, which encode the T-and t-antigens, three viral capsid proteins (VP1-3), and an agnoprotein involved in viral assembly. The T-Ag takes up over half of the viral genome [4]. With most humans subclinically infected during childhood, this virus is ubiquitous.…”

Presence of JC Virus DNA in the Tumor Tissue and Normal Mucosa of Patients with Sporadic Colorectal Cancer (CRC) or with Positive Family History and Bethesda Criteria

“…It encodes the viral early proteins, T-and t-antigens, and the viral late capsid proteins VP1, VP2, and VP3, and accessory agnoprotein [1]. Virtually all human populations are exposed to this virus within the first 5 years of life, and most of the adult population has an antibody titer against JCV [2,3].…”

Association between hMLH1 hypermethylation and JC virus (JCV) infection in human colorectal cancer (CRC)

“…Structurally, JCV contains only six genes which encode the T- and t-antigens, three viral capsid proteins (VP1-3), and an agnoprotein involved in viral assembly. The T-Ag takes up over half of the viral genome (Raj et al 1998). With most humans subclinically infected during childhood, this virus is ubiquitous.…”