T-6b allocates more assimilation product for oil synthesis and less for polysaccharide synthesis during the seed development of Arabidopsis thaliana

Jin, Yunkai; Hu, Jia; Liu, Xun; Ruan, Ying; Sun, Chuanxin; Liu, Chunlin

doi:10.1186/s13068-017-0706-3

Cited by 6 publications

(9 citation statements)

References 61 publications

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“…Thin Layer Chromatography analysis and oil content determination on Gas Chromatography (GC) were performed according to Aslan et al (2015) and Jin et al (2017b).…”

Section: Thin Layer Chromatography Analysis and Oil Content Determinamentioning

confidence: 99%

Adaptation of Rice to the Nordic Climate Yields Potential for Rice Cultivation at Most Northerly Site and the Organic Production of Low-Arsenic and High-Protein Rice

Fei

Jin

et al. 2020

Front. Plant Sci.

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There is an urgent demand for low-arsenic rice in the global market, particularly for consumption by small children. Soils in Uppsala, Sweden, contain low concentrations of arsenic (As). We hypothesize that if certain japonica paddy rice varieties can adapt to the cold climate and long day length in Uppsala and produce normal grains, such a variety could be used for organic production of low-arsenic rice for safe rice consumption. A japonica paddy rice variety, "Heijing 5," can be cultivated in Uppsala, Sweden, after several years' adaptation, provided that the rice plants are kept under a simple plastic cover when the temperature is below 10 • C. Uppsala-adapted "Heijing 5" has a low concentration of 0.1 mg per kg and high protein content of 12.6% per dry weight in brown rice grain, meaning that it thus complies with all dietary requirements determined by the EU and other countries for small children. The high protein content is particularly good for small children in terms of nutrition. Theoretically, Uppsala-adapted "Heijing 5" can produce a yield of around 5100 kg per ha, and it has a potential for organic production. In addition, we speculate that cultivation of paddy rice can remove nitrogen and phosphorus from Swedish river water and reduce nutrient loads to the Baltic Sea and associated algae blooms.

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“…Thin Layer Chromatography analysis and oil content determination on Gas Chromatography (GC) were performed according to Aslan et al (2015) and Jin et al (2017b).…”

Section: Thin Layer Chromatography Analysis and Oil Content Determinamentioning

confidence: 99%

Adaptation of Rice to the Nordic Climate Yields Potential for Rice Cultivation at Most Northerly Site and the Organic Production of Low-Arsenic and High-Protein Rice

Fei

Jin

et al. 2020

Front. Plant Sci.

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“…The fast development of nanomaterials in the quest for green, eco-friendly routes for new products often culminates in the utilization of microorganisms 1 and plant biomasses for the manufacture of sustainable nanocomposites 2 , 3 and nanoparticles, 4 – 6 which are frequently used in biological approaches. 7 In this scenario, the sweet orange ( Citrus sinensis ) presents various possibilities of biomass utilization since, after juice extraction, the remaining 50% of the fruit material is composed of peel, albedo, seeds, and bagasse.…”

Section: Introductionmentioning

confidence: 99%

Bio-based synthesis of silver nanoparticles from orange waste: effects of distinct biomolecule coatings on size, morphology, and antimicrobial activity

Barros¹,

Cruz²,

Mayrink³

et al. 2018

NSA

102

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PurposeDespite the numerous reports on biological syntheses of silver nanoparticles (AgNPs), little is known about the composition of their capping agents, protein corona of plant extract-mediated synthesis, and their influence on the properties of AgNPs. Here, orange (Citrus sinensis) waste was utilized as a source of an extract for AgNP synthesis (the protein corona composition of which was elucidated), and also as a starting material for hesperidin and nanocellulose extraction, which were used for bio-based AgNP synthesis. A comparison of the results using the two methods of synthesis is presented.MethodsAgNPs were synthesized using orange (C. sinensis) peel extract (Or-AgNPs) in a biological route, and using hesperidin (Hsd-AgNPs) and nanocellulose (extracted from oranges) in a green chemical route. Characterization of nanoparticles was carried out using zeta potential and hydrodynamic size measurements, transmission electron microscopy, and X-ray diffraction. Elucidation of proteins from protein corona was performed via ultra performance liquid chromatography-tandem mass spectrometer experiments. Antimicrobial activity was assessed via minimum inhibitory concentration assays against Xanthomonas axonopodis pv. citri (Xac), the bacterium that causes citric canker in oranges.ResultsOr-AgNPs were not completely uniform in morphology, having a size of 48.1±20.5 nm and a zeta potential of −19.0±0.4 mV. Stabilization was performed mainly by three proteins, which were identified by tandem mass spectrometry (MS/MS) experiments. Hsd-AgNPs were smaller (25.4±12.5 nm) and had uniform morphology. Nanocellulose provided a strong steric and electrostatic (−28.2±1.0 mV) stabilization to the nanoparticles. Both AgNPs presented roughly the same activity against Xac, with the minimum inhibitory concentration range between 22 and 24 μg mL−1.ConclusionDespite the fact that different capping biomolecules on AgNPs had an influence on morphology, size, and stability of AgNPs, the antibacterial activity against Xac was not sensitive to this parameter. Moreover, three proteins from the protein corona of Or-AgNPs were identified.

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“…To our best knowledge, this would be the first report on engineering SI in sorghum, or any other cereal. Sweet sorghum has been considered as a biofuel and biomass crop [ 13 ]. Our results displayed that sugar content can be increased up to 69% in hybrids compared with sweet sorghum, which will bfoost industrial value at large scale.…”

Section: Discussionmentioning

confidence: 99%

“…Among sugar crops, sugarcane accounts for almost 80% of global sugar production. Sweet sorghum has displayed huge potential to be multiple sources of energy, food and animal feed and could be a substitute for sugarcane to produce biofuel [ 13 , 14 ]. It grows quickly in adverse stress conditions of marginal lands in tropical, subtropical and temperate zones.…”

Section: Introductionmentioning

confidence: 99%

“…It is a C 4 , drought tolerant, high biomass, and high water use efficiency crop that produces stalks up to five meters tall, accumulating sucrose (α-D-glucopyranosyl-1,2-D-fructofuranose). However, current sweet sorghum varieties, producing comparatively low sugar content (around 500 mmol/L), urgently require plant breeders to improve sugar accumulation in stalks for biofuel [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum

Liu

Zhang

Gong

et al. 2021

Biotechnol Biofuels

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Background Sugar content is critically important in determining sugar crop productivity. However, improvement in sugar content has been stagnant among sugar crops for decades. Sorghum, especially sweet sorghum with high biomass, shown great potential for biofuel, has lower sugar content than sugarcane. To enhance sugar content, the sucrose isomerase (SI) gene, driven by stem-specific promoters (A2 or LSG) with a vacuole-targetted signal peptide, was transformed into the sorghum inbred line (T×430). Results The study demonstrated that transgenic lines of grain sorghum, containing 50–60% isomaltulose, accumulated up to eightfold (1000 mM) more total sugar than the control T×430 did (118 mM) in stalks of T0 generation. Subsequently, the elite engineered lines (A5, and LSG9) were crossed with sweet sorghum (Rio, and R9188). Total sugar contents (over 750 mM), were notably higher in F1, and F2 progenies than the control Rio (480 mM). The sugar contents of the engineered lines (over 750 mM), including T0, T1, F1, and F2, are surprisingly higher than that of the field-grown sugarcane (normal range 600–700 mmol/L). Additionally, analysis of physiological characterization demonstrated that the superior progenies had notably higher rates of photosynthesis, sucrose transportation, and sink strength than the controls. Conclusions The genetic engineering approach has dramatically enhanced total sugar content in grain sorghum (T0, and T1) and hybrid sorghum (F1, and F2), demonstrating that sorghum can accumulate as high or higher sugar content than sugarcane. This research illustrates that the SI gene has enormous potential on improvement of sugar content in sorghum, particularly in hybirds and sweet sorghum. The substantial increase on sugar content would lead to significant financial benefits for industrial utilization. This study could have a substantial impact on renewable bioenergy. More importantly, our results demonstrated that the phenotype of high sugar content is inheritable and shed light on improvement for other sugar crops.

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T-6b allocates more assimilation product for oil synthesis and less for polysaccharide synthesis during the seed development of Arabidopsis thaliana

Cited by 6 publications

References 61 publications

Adaptation of Rice to the Nordic Climate Yields Potential for Rice Cultivation at Most Northerly Site and the Organic Production of Low-Arsenic and High-Protein Rice

Adaptation of Rice to the Nordic Climate Yields Potential for Rice Cultivation at Most Northerly Site and the Organic Production of Low-Arsenic and High-Protein Rice

Bio-based synthesis of silver nanoparticles from orange waste: effects of distinct biomolecule coatings on size, morphology, and antimicrobial activity

Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum

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