Szabadgyök-felszabadulás vizsgálata femtoszekundum                     lézerrel asszisztált capsulotomiát követően

Tóth, Gábor; Sándor, Gábor László; Kleiner, Dénes; Szentmáry, Nóra; Kiss, Huba; Blázovics, Anna; Nagy, Zoltán Zsolt

doi:10.1556/650.2016.30604

Cited by 2 publications

(1 citation statement)

References 25 publications

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“…A vizsgálati szövetet a műtét során mechanikusan eltávolított elülső lencsetok és a szorosan rajta fekvő egyrétegű lencseepithel képezte. A minták, melyek femtoszekundumos lézerrel asszisztált műtétből származnak, a vizsgálatba nem kerültek bele [21,22]. Kétféle vizsgálómódszert alkalmaztunk: morfológiai vizsgálatokra transzmissziós elektronmikroszkópiát (TEM) és molekuláris vizsgálatot, fehérjekimutatást (caveolin-1, cavin-1) immunhisztokémiával.…”

Section: Módszerunclassified

Caveolák szerepe a szürke hályog képződésében: humán szemlencse epithelsejtjeinek vizsgálata

et al. 2019

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Inrtoduction: Caveolae are flask shaped with 50–100 nm size, non-clathrin associated invaginations of the plasmamembrane. The main membrane protein of the structures is caveolin-1. Caveolae play an important role in numerous cellular functions including vesicular transport and cell-cycle regulation, and create platforms for classical and alternative signaling pathways. According to international studies, caveolae may influence the physiology and pathology of lens epithelial cells. Aim: The aim of the study was to examine and compare the morphology of caveolae and the immunohistochemical difference of caveolin-1 in control (myopic and hyperopic) lens epithelial cells and human lens epithelial cells affected by cataract. Authors investigated whether caveolae might have a role in cataractogenesis. Method: Anterior lens capsules were obtained by capsulorhexis during surgery of senile cataract and refractive surgery of the clear lens. Ultra-fine sections have been studied by transmission electron microscopy, and semi-fine samples were labelled for immunohistochemistry with polyclonal caveolin-1 and cavin-1 antibodies. Results: By immunohistochemistry, in the control group, significant caveolin-1 label with low cavin-1 signal were measured in the lens epithelial cells. In the cataract group high cavin-1 and caveolin-1 expression was detected. In the control group, caveolae were not observed, but in the lens epithelial cells with cataract, increased number of caveolae have been detected by electron microscopy. Conclusions: For the development and maintenance of the specific caveolae shape, caveolin-1 is needed to be accompanied by cavin-1. Therefore, it is presumable that the increased expression of cavin-1 could explain the higher number of caveolae in the cataract group. These results might suggest that caveolae might play a role in cataractogenesis. Orv Hetil. 2019; 160(8): 300–308.

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Section: Módszerunclassified

Caveolák szerepe a szürke hályog képződésében: humán szemlencse epithelsejtjeinek vizsgálata

et al. 2019

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Endothelial cell density and corneal graft thickness following excimer laser vs. femtosecond laser-assisted penetrating keratoplasty—a prospective randomized study

Tóth

Butskhrikidze

Seitz

et al. 2019

Graefes Arch Clin Exp Ophthalmol

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Purpose The study aims to compare the impact of non-mechanical excimer laser-assisted (EXCIMER) and femtosecond laserassisted (FEMTO) trephinations on graft endothelial cell density (ECD) and graft thickness before and after suture removal following penetrating keratoplasty (PK). Methods The inclusion criteria for this prospective, randomized, clinical study were as follows: (1) surgeries performed by one surgeon; (2) primary central PK; (3) keratoconus (KC) or Fuchs' dystrophy (FUCHS); (4) no previous intraocular surgery; (5) graft oversize, 0.1 mm; and (6) 16-bite double-running suture. In 68 eyes of 68 patients (mean age: 53.3 ± 19.8 years), PK was performed using either 193-nm MEL70 excimer laser (BEXCIMER^: 17 KC, 18 FUCHS) or 60-KHz femtosecond laser (BFEMTO^: 17 KC, 16 FUCHS) trephination. Specular microscopy (EM 3000) and pachymetry (EM 3000; Pentacam HR; Casia SS-1000) were performed before removing the first suture (11.4 ± 1.9 months) and after removing the second suture (22.6 ± 3.8 months), but before any additional ophthalmic surgery. Results ECD did not differ significantly (P ≥ 0.436) between EXCIMER and FEMTO either with Ball-sutures-in^(1887 ± 409 vs. 1886 ± 438) or with Ball-sutures-out^(1703 ± 379 vs. 1737 ± 362). Central corneal thickness and corneal thickness at the thinnest point of the cornea did not differ significantly between EXCIMER and FEMTO either with all-sutures-in (P ≥ 0.096 and P ≥ 0.653) or with all-sutures-out (P ≥ 0.636 and P ≥ 0.717). Conclusions EXCIMER and FEMTO trephinations from the epithelial side seem to have no disadvantages regarding endothelial cell loss after PK, and both surgical procedures are safe for the endothelium. A larger sample size and longer follow-up are needed to evaluate the long-term impact of EXCIMER and FEMTO trephinations on ECD.

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Szabadgyök-felszabadulás vizsgálata femtoszekundum lézerrel asszisztált capsulotomiát követően

Cited by 2 publications

References 25 publications

Caveolák szerepe a szürke hályog képződésében: humán szemlencse epithelsejtjeinek vizsgálata

Caveolák szerepe a szürke hályog képződésében: humán szemlencse epithelsejtjeinek vizsgálata

Endothelial cell density and corneal graft thickness following excimer laser vs. femtosecond laser-assisted penetrating keratoplasty—a prospective randomized study

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