Systems Pharmacology and Microbiome Dissection of Shen Ling Bai Zhu San Reveal Multiscale Treatment Strategy for IBD

Lv, Weijie; Liu, Cui; Li, Yuefei; Chen, Wenqian; Li, Zeng-quan; Xiong, Ying; Chao, Limin; Xu, Xiaolong; Guo, Shining

doi:10.1155/2019/8194804

Cited by 27 publications

(26 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, it is important to find alternative drugs for the alleviation of pneumoniainduced deaths. SLBZS is a well-known TCM formula used in China for the treatment of gastrointestinal disorders such as diarrhea, ulcerative colitis, and inflammatory bowel disease (IBD) [13,25,26]. This study showed that SLBZS or cefixime treatment decreased bacterial load in BALF, reduced wet/dry weight ratio, inhibited MPO activity and reduced neutrophils count in BALF, and ameliorated lung injury in Spn-induced pneumonia, suggesting that these treatments ameliorates lung injury in Spn-induced pneumonia mice.…”

Section: Discussionmentioning

confidence: 99%

Shen-ling-bai-zhu-san ameliorates inflammation and lung injury by increasing the gut microbiota in the murine model of Streptococcus pneumonia-induced pneumonia

Feng

Dai

Zhang

et al. 2020

BMC Complement Med Ther

View full text Add to dashboard Cite

Background: Shen-ling-bai-zhu-san (SLBZS) regulates inflammation and gut microbiota which are associated with Streptococcus pneumoniae (Spn)-induced pneumonia. So, we studied the therapeutic effect of SLBZS and evaluated whether gut microbiota is associated with the effects of SLBZS in improving Spn-induced pneumonia. Methods: Spn-induced pneumonia NIH mice were treated by SLBZS and cefixime. A CT scan was performed and Myeloperoxidase (MPO) activity in lung homogenates was determined using the MPO Colorimetric Assay Kit. Inflammation levels in lung homogenates were measured using ELISA. Bacterial load was coated on a TSAII sheep blood agar. Intestinal gut microbiota information was analyzed according to sequencing libraries. Results: SLBZS decreased bacterial load, reduced wet/dry weight ratio, inhibited myeloperoxidase activity, reduced the neutrophils count, and ameliorated lung injury. Furthermore, SLBZS inhibited interleukin (IL)-1β, IL-6, tumor necrosis factor-α, IL-2, IL-8, IL-12, and interferon-γ secretion and enhanced IL-10 secretion. These results suggest that SLBZS ameliorates lung injury in mice with Spn-induced pneumonia. Moreover, SLBZS reduced inflammatory cytokine levels in a concentration-dependent manner and increased gut microbiota abundance and diversity. After SLBZS treatment, bacteria such as Epsilonbacteraeota, Bacteroidetes, Actinobacteria, Proteobacteria, and Patescibacteria were significantly reduced, while Tenericutes and Firmicutes were significantly increased. Conclusion: SLBZS ameliorates inflammation, lung injury, and gut microbiota in mice with S. pneumoniaeinduced pneumonia.

show abstract

Section: Discussionmentioning

confidence: 99%

Shen-ling-bai-zhu-san ameliorates inflammation and lung injury by increasing the gut microbiota in the murine model of Streptococcus pneumonia-induced pneumonia

Feng

Dai

Zhang

et al. 2020

BMC Complement Med Ther

View full text Add to dashboard Cite

show abstract

“…In recent study, researchers apply the systems pharmacology method to explore the pharmacological mechanisms of SLBZS, and found the changes of IL-1β, IL-10, and TNF-α after SLBZS treatment (0.6 g/mL). IL-1β and TNF-α participated in the MAPK and NF-κB pathways, and the two pathways share crosstalk in DSS-induced UC rats [133].…”

Section: Tonifying Qi and Activating Blood Categorymentioning

confidence: 96%

Targeting NF-κB pathway for treating ulcerative colitis: comprehensive regulatory characteristics of Chinese medicines

Zhao

2020

Chin Med

View full text Add to dashboard Cite

Nuclear factor-kappa B (NF-κB) is a kind of multi-functional nuclear transcription factor involved in regulating gene transcription to influence pathological evolution of inflammatory and immune diseases. Numerous literature evidence that NF-κB pathway plays an essential role in pathogenic development of ulcerative colitis (UC). UC is a chronic non-specific inflammatory bowel disease, and until now, therapeutic agents for UC including aminosalicylates, corticosteroids and immune inhibitors still cannot exert satisfied effects on patients. In recent years, Chinese medicines suggest the advantages of alleviating symptoms and signs, decreasing side-effects and recurrence, whose one of mechanisms is related to regulation of NF-κB pathway. In this review, we categorize Chinese medicines according to their traditional therapeutic functions, and summarize the characteristics of Chinese medicines targeting NF-κB pathway in UC treatment. It indicates that 85 kinds of Chinese medicines' compounds and formulae can directly act on NF-κBp65; while 58 Chinese medicines' ingredients and formulae indirectly suppress NF-κBp65 by regulation of its upstream or other related pathways. Moreover, by the analysis of Chinese medicines' category based on their traditional functions, we conclude the category of dampness-drying and detoxificating medicine targeting NF-κB pathway accounts for primary status for amelioration of UC. Simultaneously, this review also contributes to the choices of Chinese medicine category and provides curative potential of Chinese medicines for clinical UC treatment.

show abstract

“…All networks were visualized using Cytoscape software (version 3.7.1) [88]. e target location network was generated based on the analysis of gene expression data of various hematopoietic tissues and organs obtained from e Human Protein Atlas [89] and BioGPS databases [90]; this network was generated by linking the target to the corresponding tissues and organs where it was analyzed to be specifically expressed using previously described procedures [91][92][93][94][95][96][97][98]. e protein-protein interaction (PPI) network was constructed using the STRING database (version 11.0) [99] and interactions with highest confidence scores (≥0.9) were selected for further analysis.…”

Section: Construction Of Smt-associated Networkmentioning

confidence: 99%

“…To understand the therapeutic effects of SMT at the tissue-and organ-level, we generated a target location network based on the gene expression data for individual myelosuppression-associated SMT targets across various tissues and organs, which were identified from e Human Protein Atlas [88] and BioGPS databases [90] and analyzed as previously described [91][92][93][94][95][96][97][98] (Figure 5; see Materials and Methods). Consequently, we found that the targets were expressed in various hematopoietic tissues and organs [129,130], including the liver (degree = 144), bone marrow (degree = 137), spleen (degree = 116), lymph nodes (degree = 113), and blood (degree = 100) ( Figure 5), which suggests the systematic mechanism of action responsible for the pharmacological effects of SMT.…”

Section: Network-based Analysis Of the Pharmacological Mechanisms Of mentioning

confidence: 99%

Network Pharmacology‐Based Investigation of the System‐Level Molecular Mechanisms of the Hematopoietic Activity of Samul‐Tang, a Traditional Korean Herbal Formula

Lee¹,

Park²,

Lee

2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Hematopoiesis is a dynamic process of the continuous production of diverse blood cell types to meet the body’s physiological demands and involves complex regulation of multiple cellular mechanisms in hematopoietic stem cells, including proliferation, self-renewal, differentiation, and apoptosis. Disruption of the hematopoietic system is known to cause various hematological disorders such as myelosuppression. There is growing evidence on the beneficial effects of herbal medicines on hematopoiesis; however, their mechanism of action remains unclear. In this study, we conducted a network pharmacological-based investigation of the system-level mechanisms underlying the hematopoietic activity of Samul-tang, which is an herbal formula consisting of four herbal medicines, including Angelicae Gigantis Radix, Rehmanniae Radix Preparata, Paeoniae Radix Alba, and Cnidii Rhizoma. In silico analysis of the absorption-distribution-metabolism-excretion model identified 16 active phytochemical compounds contained in Samul-tang that may target 158 genes/proteins associated with myelosuppression to exert pharmacological effects. Functional enrichment analysis suggested that the targets of Samul-tang were significantly enriched in multiple pathways closely related to the hematopoiesis and myelosuppression development, including the PI3K-Akt, MAPK, IL-17, TNF, FoxO, HIF-1, NF-kappa B, and p53 signaling pathways. Our study provides novel evidence regarding the system-level mechanisms underlying the hematopoiesis-promoting effect of herbal medicines for hematological disorder treatment.

show abstract

Systems Pharmacology and Microbiome Dissection of Shen Ling Bai Zhu San Reveal Multiscale Treatment Strategy for IBD

Cited by 27 publications

References 87 publications

Shen-ling-bai-zhu-san ameliorates inflammation and lung injury by increasing the gut microbiota in the murine model of Streptococcus pneumonia-induced pneumonia

Shen-ling-bai-zhu-san ameliorates inflammation and lung injury by increasing the gut microbiota in the murine model of Streptococcus pneumonia-induced pneumonia

Targeting NF-κB pathway for treating ulcerative colitis: comprehensive regulatory characteristics of Chinese medicines

Network Pharmacology‐Based Investigation of the System‐Level Molecular Mechanisms of the Hematopoietic Activity of Samul‐Tang, a Traditional Korean Herbal Formula

Contact Info

Product

Resources

About