Systems Glycobiology: Integrating Glycogenomics, Glycoproteomics, Glycomics, and Other ‘Omics Data Sets to Characterize Cellular Glycosylation Processes

Industrial bioprocessing perspectives on managing therapeutic protein charge variant profiles

“…Sialic acid modifications not only impact therapeutic efficacy but also change the net charge of the protein. Since the publication of the CHO genome (Lewis et al, ), omics‐based approaches have successfully modified cell line productivity and quality (Bennun et al, ; Kuo et al, ; Lewis, Abu‐Absi, Borys, & Li, ). Recent works have shaped glycan profile by modifying glycosyltransferase activity (Yang, Wang, et al, ), sialyltransferase activity (Chung, Wang, Yang, Ponce, et al, ; Chung, Wang, Yang, Yin, et al, ; Könitzer et al, ), and lectin binding preference (Zhang, Luo, & Zhang, ) and knocking out GDP‐4‐keto‐6‐d‐deoxymannose epimerase/GDP‐4‐keto‐6‐l‐galactose reductase (FX) (Louie et al, ).…”

Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions

“…Moreover, glycosylation is probably the most important quality attribute of therapeutic glycoproteins, because the pharmacokinetic effects of undesired glycosylation patterns can be decreased drug efficacy or increased antigenicity (Bertozzi et al, 2009;Butler and Spearman, 2014). Recent advances in glycoengineering and descriptions of how effector genes can be used to modulate glycosylation have been described in recent reviews (Bennun et al, 2016;Dicker and Strasser, 2015;Spahn and Lewis, 2014). Because of scope limitations, only misfolding/aggregation and propeptide processing in relation to effector genes will be described here.…”

Revisiting the Language of Glycoscience: Readers, Writers and Erasers in Carbohydrate Biochemistry

“…A failure to use words clearly undermines the global response to antimicrobials′ waning usefulness” . [ 5] Technological and informatics advances in glycoscience, alongside combinations of the two, are providing new ways to cut through the complexity, whilst comprehensive books of glycobiology topics provide entries in to the field . The introduction of stylized symbol nomenclature for glycans (SNFG; Figure ) also represents an important step towards simplifying communication within and between interested disciplines along with guidelines for experimental design and data curation, and a repository for glycan structures …”

“…Af ailure to use words clearly underminest he global response to antimicrobials' waning usefulness". [5] Te chnological [6] and informatics [7] advancesi ng lycoscience, alongside combinations of the two, [8] are providing new ways to cut through the complexity,w hilst [a] Dr.The ORCID identification numbers for the authors of this article can be found under https://doi.org/10.1002/cbic.201900377.The roles of carbohydrates in nature are many and varied. However,the lack of templatee ncoding in glycoscience distances carbohydrate structure, and hence function, from gene sequence.…”