Systems biology of antioxidants

Andriantsitohaina, Ramaroson; Duluc, Lucie; García-Rodríguez, Julio César; Valle, Lizette Gil del; Guevara-García, Mariela; Simard, Gilles; Soleti, Raffaella; Su, Ding‐Feng; Velásquez-Pérez, Luis; Wilson, John X.; Laher, Ismail

doi:10.1042/cs20110643

Cited by 32 publications

(21 citation statements)

References 133 publications

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“…The cellular injury observed in sepsis produces highly reactive biochemical intermediates from nitrogen and oxygen [35]. Following an injury, we now know that the increased production of reactive oxygen species (ROS) and the increased activity of clearance mechanisms do not necessarily indicate cell damage [35,36].…”

Section: Introductionmentioning

confidence: 99%

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The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis

et al. 2014

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BackgroundSepsis is a prevalent condition in critically ill patients and may be associated with thiamine deficiency (TD). The aim of this study was to evaluate the effect of TD on inflammation, oxidative stress and cellular recruitment in a sepsis model.MethodsThe experimental sepsis model, cecal ligation and puncture (CLP), was utilized on mice in comparison with a sham procedure. The following four groups were compared against each other: SHAM with AIN93G complete chow, SHAM with thiamine deficient (TD) chow, CLP with AIN93G complete chow, and CLP with TD chow. Thiamine pyrophosphate (TPP) blood concentrations were determined, and blood and peritoneal fluid were evaluated for differences in TNF-alpha, IL-1, IL-6, KC and MCP-1/CCL2 levels. In addition, the levels of 4-HNE adducts in liver proteins were evaluated by Western Blot.ResultsThe mean TPP blood concentration from the mice fed with the complete chow was 303.3 ± 42.6 nmol/L, and TD occurred within 10 days. TNF-α and MCP-1 concentrations in the peritoneal fluid were significantly greater in the CLP with TD chow group when compared with the other groups. The blood IL-1β level, however, was lower in the CLP with TD chow group. Liver 4-HNE levels were highest in the TD chow groups. Blood mononuclear cell numbers, as well as peritoneal total leukocyte, mononuclear cell and neutrophil numbers were greater in the CLP with TD chow group. Peritoneal bacterial colony forming units (CFU) were significantly lower in the CLP with TD chow group.ConclusionTD was associated with greater bacterial clearance, oxidative stress and inflammatory response changes.

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Section: Introductionmentioning

confidence: 99%

“…Following an injury, we now know that the increased production of reactive oxygen species (ROS) and the increased activity of clearance mechanisms do not necessarily indicate cell damage [35,36]. ROS have the ability to react with proteins, lipids, and genetic material.…”

Section: Introductionmentioning

confidence: 99%

The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis

et al. 2014

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“…Several compounds including quercetin, fisetin and DiOHF (3′,4′-dihydroxyflavonol) have been investigated in our laboratory and by others for the treatment of ischaemic stroke, with promising results [64,65]. Numerous additional agents that display antioxidant properties and have been used in an ischaemic stroke setting, including other phenolic compounds and vitamins, are described in precedent reviews [66,67,68,69,70,71,72,73]. …”

Section: Antioxidants For Strokementioning

confidence: 99%

NADPH Oxidase as a Therapeutic Target for Neuroprotection against Ischaemic Stroke: Future Perspectives

McCann

Roulston

2013

Brain Sciences

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Oxidative stress caused by an excess of reactive oxygen species (ROS) is known to contribute to stroke injury, particularly during reperfusion, and antioxidants targeting this process have resulted in improved outcomes experimentally. Unfortunately these improvements have not been successfully translated to the clinical setting. Targeting the source of oxidative stress may provide a superior therapeutic approach. The NADPH oxidases are a family of enzymes dedicated solely to ROS production and pre-clinical animal studies targeting NADPH oxidases have shown promising results. However there are multiple factors that need to be considered for future drug development: There are several homologues of the catalytic subunit of NADPH oxidase. All have differing physiological roles and may contribute differentially to oxidative damage after stroke. Additionally, the role of ROS in brain repair is largely unexplored, which should be taken into consideration when developing drugs that inhibit specific NADPH oxidases after injury. This article focuses on the current knowledge regarding NADPH oxidase after stroke including in vivo genetic and inhibitor studies. The caution required when interpreting reports of positive outcomes after NADPH oxidase inhibition is also discussed, as effects on long term recovery are yet to be investigated and are likely to affect successful clinical translation.

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“…and reactive nitrogen species (RNS; nitrogen dioxide CNO 2 , peroxynitrite ONOO À , etc.) responsible for the oxidation of several target molecules, including proteins, cellular membranes, cells, DNA or RNA play a relevant role in the development of several diseases such as Alzheimer, Parkinson and cancer [1][2][3][4][5][6][7]. For instance, it has been shown recently that the oxidation of copper (I) ions complexed with b-amyloid peptides leads to the formation of b-amyloid agglomerates which precipitate as plaques in the brain and contribute to the generation of Alzheimer diseases [8].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Pseudo‐Titration of Water‐Soluble Antioxidants

et al. 2013

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An amperometric test for the antioxidant power (AOP) of biological and food samples is presented. The gist of the method is to measure by linear sweep voltammetry the anodic current produced during the oxidation of the labile species present in the sample and then rationalizing this signal with a mathematical treatment that allows the pseudo-titration of antioxidants (AOs) around a given threshold potential. As a result, the AOP of the sample is calculated. This method allows the discrimination of the most biologically relevant AOs that react rapidly and at low oxidation potentials, from less reactive AOs that are oxidised slowly and at much higher oxidation potentials. This methodology was applied for measuring the AOP of blood, saliva, and natural drinks like orange juice.

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Systems biology of antioxidants

Cited by 32 publications

References 133 publications

The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis

The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis

NADPH Oxidase as a Therapeutic Target for Neuroprotection against Ischaemic Stroke: Future Perspectives

Electrochemical Pseudo‐Titration of Water‐Soluble Antioxidants

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