Systems biology approach delineates critical pathways associated with disease progression in rheumatoid arthritis

Bhagavatham, Sai Krishna Srimadh; Pulukool, Sujith Kumar; Pradhan, Sai Sanwid; Saiswaroop, R.; Naik, Ashwin Ashok; M., Datta Darshan V.; Sivaramakrishnan, Venketesh

doi:10.1080/07391102.2022.2115555

Cited by 9 publications

(1 citation statement)

References 158 publications

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“…TNF-α is a cytokine that initiates immune-mediated inflammation response . It is an important biomarker in diseases like congenital heart disease, dengue, rheumatoid arthritis, glaucoma, − to name a few. To study its regulation, enzyme linked immuno-sorbent assay (ELISA) is utilized as a well-known, reliable, and gold-standard plate-based technique.…”

Section: Resultsmentioning

confidence: 99%

Tecoma stans Floral Extract-Based Biosynthesis for Enhanced Surface Plasmon-Coupled Emission and a Preliminary Study on Fluoroimmunoassay

Rathnakumar,

Bhaskar,

Sivaramakrishnan

et al. 2024

Anal. Chem.

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We demonstrate the synthesis of biogenic supported silver spiked star architectures and their application to increase the electromagnetic field intensity at its tips that enhance plasmoncoupled emission. Tecoma stans floral extract has been used to synthesize silver nanocubes and spiked stars. We observe ∼445-fold and ∼680-fold enhancements in spacer and cavity configurations, respectively, in the SPCE platform. The hotspot intensity and Purcell factor are evaluated by carrying out finite-difference time-domain (FDTD) simulations. Time-based studies are presented to modulate the sharpness of the edges wherein an increase in the tip sharpness with the increase in reaction time up to 5 h is observed. The unique morphology of the silver architectures allowed us to utilize them in biosensing application. A SPCE-based fluoroimmunoassay was performed, achieving a 1.9 pg/mL limit of detection of TNF-α cytokine. This combination of anisotropic architectures, SPCE and immunoassay prove to be a powerful platform for the ultrasensitive detection of biomarkers in surface-bound assays.

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