Systemic treatment with adipose-derived mesenchymal stem cells ameliorates clinical and pathological features in the amyotrophic lateral sclerosis murine model

Marconi, Silvia; Bonaconsa, Marta; Scambi, Ilaria; Squintani, Giovanna; Wei, Rui; Turano, Ermanna; Ungaro, D.; D’Agostino, Stefania; Barbieri, Franco; Angiari, Stefano; Farinazzo, Alessia; Constantin, Gabriela; Carro, Ubaldo Del; Bonetti, Bruno; Mariotti, Raffaella

doi:10.1016/j.neuroscience.2013.05.034

Cited by 127 publications

(106 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the last decade, several studies have demonstrated that the therapeutic potential of ASCs could be due to their paracrine activity rather than to their integration and survival in host tissues. [4][5][6] In particular, the paracrine mechanism seems to be mediated through exosomes release, making exosomes a potential alternative to cell therapy in neurodegenerative diseases.…”

Section: Discussionmentioning

confidence: 99%

“…[1][2][3] Moreover, it has been recently demonstrated that in vivo administration of ASCs ameliorates the disease condition in several neurodegenerative animal models. [4][5][6] In particular, it has been reported that transplantation of autologous ASCs has a therapeutic effect in experimental autoimmune encephalomyelitis and in murine model of familial amyotrophic…”

Section: Introductionmentioning

confidence: 99%

“…5,6 Literature findings indicate that ASCs exert their action through paracrine activity rather than through engraftment. [7][8][9] Furthermore, this paracrine activity is mediated by the release of membrane vesicles, such as exosomes.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Busato

Bonafede

Bontempi

et al. 2016

IJN

View full text Add to dashboard Cite

Purpose Recent findings indicate that the beneficial effects of adipose stem cells (ASCs), reported in several neurodegenerative experimental models, could be due to their paracrine activity mediated by the release of exosomes. The aim of this study was the development and validation of an innovative exosome-labeling protocol that allows to visualize them with magnetic resonance imaging (MRI). Materials and methods At first, ASCs were labeled using ultrasmall superparamagnetic iron oxide nanoparticles (USPIO, 4–6 nm), and optimal parameters to label ASCs in terms of cell viability, labeling efficiency, iron content, and magnetic resonance (MR) image contrast were investigated. Exosomes were then isolated from labeled ASCs using a standard isolation protocol. The efficiency of exosome labeling was assessed by acquiring MR images in vitro and in vivo as well as by determining their iron content. Transmission electron microscopy images and histological analysis were performed to validate the results obtained. Results By using optimized experimental parameters for ASC labeling (200 µg Fe/mL of USPIO and 72 hours of incubation), it was possible to label 100% of the cells, while their viability remained comparable to unlabeled cells; the detection limit of MR images was of 10 2 and 2.5×10 3 ASCs in vitro and in vivo, respectively. Exosomes isolated from previously labeled ASCs retain nanoparticles, as demonstrated by transmission electron microscopy images. The detection limit by MRI was 3 µg and 5 µg of exosomes in vitro and in vivo, respectively. Conclusion We report a new approach for labeling of exosomes by USPIO that allows detection by MRI while preserving their morphology and physiological characteristics.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Busato

Bonafede

Bontempi

et al. 2016

IJN

View full text Add to dashboard Cite

show abstract

“…Marconi et al assessed the efficacy of the systemic administration of adipose-derived mesenchymal stem cells (ASC) in SOD1-mutant mice and found that the cells not only significantly delayed motor deterioration for 4-6 weeks and maintained the number of motor neurons but also up-regulated the levels of glialderived neurotrophic factor (GDNF) and basic fibroblast growth factor (bFGF) in the spinal cord. Given that ASCs produce bFGF but not GDNF, these findings indicated that ASCs may promote neuroprotection either directly and/or by modulating the response of local glial cells toward a neuroprotective phenotype [40]. Similarly, intramuscular transplantation of MSCs engineered to secrete GDNF was found to attenuate motor neuron loss and prolong the lifespan of ALS rats [41].…”

Section: Mesenchymal Stromal Cellsmentioning

confidence: 99%

Stem Cell Therapy for Amyotrophic Lateral Sclerosis

Chen

Feldman

2017

Molecular and Cellular Therapies for Motor Neuron Diseases

View full text Add to dashboard Cite

“…Embryonic Stem Cells: By using embryonic stem cells (ES cells) from mice carrying transgenic alleles of SOD1 gene to generate motor neurons in vitro [50], in 2011 Wyatt TJ et al, transplanted these ES cells into SOD1G93A rodent model of ALS results in enhancing the numbers of endogenous neurons [51].…”

Section: In-vitro Trials Involving Mechanisms Of Stem Cell-mentioning

confidence: 99%

Stem Cells for Neuro-regeneration: State of the Art

Wagih¹,

Elhawary²,

Ellessy³

et al. 2015

BIO

View full text Add to dashboard Cite

Neuroregeneration (NR) is a long-sought medical dream which has intrigued vast research in the past decades. A traditional physiologic dogma that central nervous system does not regenerate has been strongly challenged in the recent years since the advent of the stem cell era. Stem cell research in the regenerative field passes through three main stages. The first stage is the in-vitro experiments which studies the exact molecular and cellular mechanisms underlying stem cell-mediated NR. The second stage is the application of these data in experimental animal settings to provide "proof of concept" of stem cell therapy in animal models. The final step is the translation of these data in pilot clinical trials. In this review, we will try to gather the different data of stem cell-mediated NR from various experimental and clinical researches.

show abstract

Systemic treatment with adipose-derived mesenchymal stem cells ameliorates clinical and pathological features in the amyotrophic lateral sclerosis murine model

Cited by 127 publications

References 49 publications

Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Stem Cell Therapy for Amyotrophic Lateral Sclerosis

Stem Cells for Neuro-regeneration: State of the Art

Contact Info

Product

Resources

About