Systemic Treatment of Acute Liver Failure with Adipose Derived Stem Cells

Migueláñez, Isabel Pascual; Salinas-Gómez, Javier; Fernández-Luengas, D.; Villar-Zarra, Karen; Clemente, Luz Vega; García‐Arranz, Mariano; Olmo, Damián García

doi:10.3109/08941939.2014.987407

Cited by 17 publications

(11 citation statements)

References 29 publications

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“…Considerably, compared with those without ADSC injection, less incidences of inflammatory infiltration were appeared around the liver central vein of treated mice after tail intravenous injection of the SPIONs cluster@PDA-labeled ADSCs for 24 hrs, implying that the labeled ADSCs could promote the repair of injured liver. These results are in well consistent with the previous studies that unlabeled ADSCs can ameliorate the liver injuries induced by CCl 4 in rodent model 39 40 41 42 . Hence, above data indicate the therapeutic effects of ADSCs on liver injures cannot be affected by the labeling of SPIONs cluster@PDA nanoparticles, and also proved the good biocompatibility of these nanoparticles.…”

Section: Resultssupporting

confidence: 93%

Poly (dopamine) coated superparamagnetic iron oxide nanocluster for noninvasive labeling, tracking and targeted delivery of adipose tissue-derived stem cells

Liao

Fan

et al. 2016

Sci Rep

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Tracking and monitoring of cells in vivo after transplantation can provide crucial information for stem cell therapy. Magnetic resonance imaging (MRI) combined with contrast agents is believed to be an effective and non-invasive technique for cell tracking in living bodies. However, commercial superparamagnetic iron oxide nanoparticles (SPIONs) applied to label cells suffer from shortages such as potential toxicity, low labeling efficiency, and low contrast enhancing. Herein, the adipose tissue-derived stem cells (ADSCs) were efficiently labeled with SPIONs coated with poly (dopamine) (SPIONs cluster@PDA), without affecting their viability, proliferation, apoptosis, surface marker expression, as well as their self-renew ability and multi-differentiation potential. The labeled cells transplanted into the mice through tail intravenous injection exhibited a negative enhancement of the MRI signal in the damaged liver-induced by carbon tetrachloride, and subsequently these homed ADSCs with SPIONs cluster@PDA labeling exhibited excellent repair effects to the damaged liver. Moreover, the enhanced target-homing to tissue of interest and repair effects of SPIONs cluster@PDA-labeled ADSCs could be achieved by use of external magnetic field in the excisional skin wound mice model. Therefore, we provide a facile, safe, noninvasive and sensitive method for external magnetic field targeted delivery and MRI based tracking of transplanted cells in vivo.

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Section: Resultssupporting

confidence: 93%

Poly (dopamine) coated superparamagnetic iron oxide nanocluster for noninvasive labeling, tracking and targeted delivery of adipose tissue-derived stem cells

Liao

Fan

et al. 2016

Sci Rep

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“…(3) ERC-treated group: mice first receiving i.p. injection of CCl 4 were injected intravenously with 1 × 10 6 ERCs at passage 4 resuspended in 200 μl of PBS 30 min later [ 23 ]. Mice were sacrificed 24 h after injection of CCl 4 , and blood was collected.…”

Section: Methodsmentioning

confidence: 99%

Human endometrial regenerative cells alleviate carbon tetrachloride-induced acute liver injury in mice

Shi

et al. 2016

J Transl Med

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BackgroundThe endometrial regenerative cell (ERC) is a novel type of adult mesenchymal stem cell isolated from menstrual blood. Previous studies demonstrated that ERCs possess unique immunoregulatory properties in vitro and in vivo, as well as the ability to differentiate into functional hepatocyte-like cells. For these reasons, the present study was undertaken to explore the effects of ERCs on carbon tetrachloride (CCl4)–induced acute liver injury (ALI).MethodsAn ALI model in C57BL/6 mice was induced by administration of intraperitoneal injection of CCl4. Transplanted ERCs were intravenously injected (1 million/mouse) into mice 30 min after ALI induction. Liver function, pathological and immunohistological changes, cell tracking, immune cell populations and cytokine profiles were assessed 24 h after the CCl4 induction.ResultsERC treatment effectively decreased the CCl4-induced elevation of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and improved hepatic histopathological abnormalities compared to the untreated ALI group. Immunohistochemical staining showed that over-expression of lymphocyte antigen 6 complex, locus G (Ly6G) was markedly inhibited, whereas expression of proliferating cell nuclear antigen (PCNA) was increased after ERC treatment. Furthermore, the frequency of CD4+ and CD8+ T cell populations in the spleen was significantly down-regulated, while the percentage of splenic CD4+CD25+FOXP3+ regulatory T cells (Tregs) was obviously up-regulated after ERC treatment. Moreover, splenic dendritic cells in ERC-treated mice exhibited dramatically decreased MHC-II expression. Cell tracking studies showed that transplanted PKH26-labeled ERCs engrafted to lung, spleen and injured liver. Compared to untreated controls, mice treated with ERCs had lower levels of IL-1β, IL-6, and TNF-α but higher level of IL-10 in both serum and liver.ConclusionsHuman ERCs protect the liver from acute injury in mice through hepatocyte proliferation promotion, as well as through anti-inflammatory and immunoregulatory effects.

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“…It is assumed that the application of stem cells obtained from stromal vascular fat tissue accelerates the regenerative process by allowing for improved angiogenesis and modulation of inflammation, as has been previously observed in animal-model studies ( 14 , 15 ); however, to the best of our knowledge, this has not been demonstrated in direct connection with ALPPS approach and Sus scrofa model organism. The aim of the present study was to identify candidate genes that may be used as screening markers for monitoring the process of liver regeneration following the first stage of ALPPS.…”

Section: Introductionmentioning

confidence: 92%

Liver regeneration during the associating liver partition and portal vein ligation for staged hepatectomy procedure in Susï¿½scrofa is positively modulated by stem cells

et al. 2018

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This present study investigated the impact of the application of stem cells to liver regeneration following the first stage of associating liver partition and portal vein ligation for staged hepatectomy (ALPPS). The experiment was conducted on a pig model (n=6, 3 that did not receive application of stem cells, 3 that received application stem cells). Collected samples of liver (day 0 and 9 following surgery) were subjected to complete transcriptome sequencing. In total, 39 differentially expressed genes were found in the group without the application of the stem cells (genes of unwanted processes such as fibrosis and inflammation). In the group that did receive application of stem cells, no significantly differentially expressed genes were found, indicating a properly regenerated liver remnant. The present study therefore demonstrated, to the best of our knowledge for the first time, the positive effect of stem cells application in the liver regeneration process during ALPPS procedure in the pig model.

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Systemic Treatment of Acute Liver Failure with Adipose Derived Stem Cells

Abstract: The intravenous injection of allogenic ASCs in this model of CCl4-induced liver failure reduced the mortality in treated animals. ASCs injected in the rat tail vein were found in the liver in animals with induced acute liver failure.

Cited by 17 publications

References 29 publications

Poly (dopamine) coated superparamagnetic iron oxide nanocluster for noninvasive labeling, tracking and targeted delivery of adipose tissue-derived stem cells

Poly (dopamine) coated superparamagnetic iron oxide nanocluster for noninvasive labeling, tracking and targeted delivery of adipose tissue-derived stem cells

Human endometrial regenerative cells alleviate carbon tetrachloride-induced acute liver injury in mice

Liver regeneration during the associating liver partition and portal vein ligation for staged hepatectomy procedure in Susï¿½scrofa is positively modulated by stem cells

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