Systemic oxidative stress could predict assisted reproductive technique outcome

Abstract

Purpose Previous studies have indicated that OxS (oxidative stress) may appear as a possible reason for poor ART outcome. Our aim was to study OxS levels in both partners of couples seeking Assisted reproduction Technology (ART). Methods Altogether 79 couples were recruited. Oxidative DNA damage (8-OHdG) and lipid peroxidation (8-EPI) were measured, and clinical background and ART outcomes were recorded. Results Both OxS markers accurately reflected clincal conditions with prominent negative effects attributable to genital tract infections, endometriosis, uterine myoma and smoking. Furthermore, the level of OxS was also affected by partner's state of health. The highest 8-EPI levels were detected in both partners when biochemically detectable pregnancies did not develop into clinically detectable pregnancies (in women, 97, 8±16,7 vs 72.9±22,9, p=0.007; in men, 89.6±20,4 vs 72,1± 22,6, p=0.049). Conclusions To conclude, high grade systemix OxS in both partners may negatively affect the maintenance and outcome of pregnancy. Applying the detection of OxS in ART patients may select patients with higher success rate and/or those who require antioxidant therapy. This would lead to improvement of ART outcome as well as natural fertility.

Effect of density gradient centrifugation on reactive oxygen species in human semen

“…The reduced ROS in the lower layer relative to the unwashed samples strongly suggests that the treatment of semen by DGC does not expose motile sperm to oxidative stress. Several studies have reported that excessive ROS levels in washed semen are negatively correlated with fertilization and pregnancy rates [Zorn et al 2003;Das et al 2008;Ahelik et al 2015]. Furthermore, ROS levels were higher in unwashed semen samples of couples that did not achieve pregnancy compared to those that did [Yumura et al 2009].…”

Oxidative stress and male infertility

“…All these events negatively affect sperm parameters, 9‐15 male fertility, 12 and pregnancy outcome of their partners. Clinically, several studies have demonstrated that OS in human semen resulted in significantly poor assisted reproductive technology (ART) outcomes, such as lower fertilization rates, arrest of embryonic development, implantation failure, recurrent pregnancy loss, and lower live birth rates 16‐18 . Agarwal et al proposed the term and concept of “Male Oxidative Stress Infertility (MOSI)”, 19 and it has been determined that there are many patients with MOSI among those who were previously classified as having idiopathic male infertility 19 .…”

“…Clinically, several studies have demonstrated that OS in human semen resulted in significantly poor assisted reproductive technology (ART) outcomes, such as lower fertilization rates, arrest of embryonic development, implantation failure, recurrent pregnancy loss, and lower live birth rates. [16][17][18] Agarwal et al proposed the term and concept of "Male Oxidative Stress Infertility (MOSI)", 19 and it has been determined that there are many patients with MOSI among those who were previously classified as having idiopathic male infertility. 19 Understanding the effects of ROS on male fertile capacity, measuring OS accurately, and treating patients based on their pathophysiologies will contribute to improving male infertility outcomes.…”

The sensitivity of the DNA damage checkpoint prevents oocyte maturation in endometriosis

“…Endometriosis is a disease associated with elevated ROS, both in the follicular environment and systemically1415161718. It is also known that ROS causes DNA damage19.…”