Systemic Distribution and Elimination of Plain and with Cy3.5 Functionalized Poly(vinyl alcohol) Coated Superparamagnetic Maghemite Nanoparticles After Intraarticular Injection in Sheep In Vivo

Abstract

PVA coated and fluorescent dye (Cy3.5) functionalized vinyl alcohol/vinyl amine copolymer coated superparamagnetic iron oxide nanoparticles (SPION) were evaluated for systemic distribution and elimination after intraarticular injection in sheep. Observation was done at 3, 24, 72, and 120 hours after injection using light microscopy, fluorescent microscopy, and confocal microscopy. No pathologic influence of SPION on the tissue harvested could be seen. A significantly increased iron content could be identified in the kidneys, lymph nodes, and spleen after injection of SPION. No particles were detected in the liver, the urinary, and the gall bladder. No positive fluorescent signal could be attributed to SPION throughout the organs. Our results indicated that the iron component of the SPION is possible to be incorporated into the physiologic iron metabolism after reabsorption in the proximal tubule system of the kidney and that concentration levels of Cy3.5 are too low to be detected throughout the body.

Structure and Properties of Iron Oxide Nanoparticles Encapsulated by Phospholipids with Poly(ethylene glycol) Tails

“…Iron oxide NPs are frequently suggested in biorelated applications because they are easily metabolized or degraded in vivo. 13,14 Magnetic properties are known to be size-dependent; [15][16][17] therefore, the narrow particle size distribution is one of the imperatives of magnetic NP fabrication. Monodisperse iron oxide NPs can be prepared by thermal decomposition of iron compounds including oxygen-containing ligands such as acetylacetonates, 1,18,19 acetates, 19 or oleates 20,21 in high-boiling solvents containing surfactants.…”

Controlled synthesis of different types iron oxides nanocrystals in paraffin oil

“…In some cases, every magnetic NC becomes a single magnetic domain and shows superparamagnetic behavior when ambient temperature is above the so-called blocking temperature. Therefore, this feature makes superparamagnetic NCs very attractive for biomedical applications because the risk of forming agglomerates is negligible at room temperature and they are generally stable under air and can be metabolized or degraded in vivo [17,18]. Moreover, monodisperse FeO NCs have great potential for catalysis [19] and gas-sensor [20] applications.…”

Ex vivo effect of gold nanoparticles on porcine synovial membrane

“…Based on this and previous work it is further expected that nanoparticles leave the joint cavity in a size dependent manor either unassisted or within cells and accumulate in the reticuloendothelial system 21 , 72 . Beyond a yet to be determined size but likely in the range of 50 nm, we anticipate AuNPs to be largely retained within the synovial environment.…”