Systemic disease as a model for development of the genetic ang cell terapy technologies

Abstract

Aim. Injury using CCl4 triggers a cascade of reactions and conditions specific for systemic lesions caused by most external anthropogenic environmental factors. Conditions for obtaining a model of systemic injury of an organism have been developed for further performing of restoration technologies with the use of stem cells and cytokines. Methods. Tumor predisposed ICR mice were chosen as the model. Systemic damage was caused with multiple intraperitoneal administration of carbon tetrachloride. Results. The optimal doses and load duration, excess above which leads to irreversible changes in the body that are not amenable to self-healing, were found. Conclusions. The model obtained can be used to study various recovery technologies of systemic damages. Keywords: systemic disease, model, carbon tetrachloride.

Triggering effect of “therapeutic MSC”

“…The object was the mice, injected twice a week for 12 weeks with carbon tetrachloride in the dose of 1.5 µl of 30 % solution of CCl 4 per one gram of the bodyweight. This procedure induced a severe damage of practically the whole organism [19]. 12 weeks later the mice of experimental group were injected with MSC, in dose of 10 5 per animal.…”