Systemic delivery of miR-126 by miRNA-loaded Bubble liposomes for the treatment of hindlimb ischemia

Endo‐Takahashi, Yoko; Negishi, Yoichi; Nakamura, Akinori; Ukai, Saori; Ooaku, Kotomi; Oda, Yusuke; Sugimoto, Katsutoshi; Moriyasu, Fuminori; Takagi, Norio; Suzuki, Ryo; Maruyama, Kazuo; Aramaki, Yukihiko

doi:10.1038/srep03883

Cited by 103 publications

(79 citation statements)

References 31 publications

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“…They utilized these bubble liposomes (BLs) for miR-126 delivery for an ischemiainduced angiogenic response. BLs contains perfluoropropane gas and can be tracked using ultrasound detection method in vivo (145).…”

Section: Non Targeted Particulate Systemsmentioning

confidence: 99%

Delivery and Targeting of miRNAs for Treating Liver Fibrosis

Kumar

Mahato

2014

Pharm Res

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Liver fibrosis is a pathological condition originating from liver damage that leads to excess accumulation of extracellular matrix (ECM) proteins in the liver. Viral infection, chronic injury, local inflammatory responses and oxidative stress are the major factors contributing to the onset and progression of liver fibrosis. Multiple cell types and various growth factors and inflammatory cytokines are involved in the induction and progression of this disease. Various strategies currently being tried to attenuate liver fibrosis include the inhibition of HSC activation or induction of their apoptosis, reduction of collagen production and deposition, decrease in inflammation, and liver transplantation. Liver fibrosis treatment approaches are mainly based on small drug molecules, antibodies, oligonucleotides (ODNs), siRNA and miRNAs. MicroRNAs (miRNA or miR) are endogenous noncoding RNA of ~22 nucleotides that regulate gene expression at post transcription level. There are several miRNAs having aberrant expressions and play a key role in the pathogenesis of liver fibrosis. Single miRNA can target multiple mRNAs, and we can predict its targets based on seed region pairing, thermodynamic stability of pairing and species conservation. For in vivo delivery, we need some additional chemical modification in their structure, and suitable delivery systems like micelles, liposomes and conjugation with targeting or stabilizing the moiety. Here, we discuss the role of miRNAs in fibrogenesis and current approaches of utilizing these miRNAs for treating liver fibrosis.

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Section: Non Targeted Particulate Systemsmentioning

confidence: 99%

Delivery and Targeting of miRNAs for Treating Liver Fibrosis

Kumar

Mahato

2014

Pharm Res

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“…*Indicates P < 0.05 using a one-way analysis of variance with Tukey's post hoc test. Reprinted from Endo-Takahashi et al (2014) with permission. compatible with cellular internalization, endosomal escape, nuclear transfer, and transcription (Hama et al, 2006;Varga et al, 2005).…”

Section: Endosomal Escape Enhanced By Blsmentioning

confidence: 96%

“…The delivered miR-126 increased angiogenic gene expression and improved blood flow (Figure 8). Thus, the combination of mi-BLs and US exposure may serve as a theranostic agent with combined diagnostic and therapeutic properties (Endo-Takahashi et al, 2014).…”

Section: Microrna-loaded Blsmentioning

confidence: 99%

“…However, a vehicle for efficiently delivering therapeutic RNA to its target tissue is required for miRNA-based therapeutics (Zhang, Wang, & Gemeinhart, 2013). We therefore attempted to prepare miRNAloaded BLs (mi-BLs) and evaluated their utility using a hindlimb ischemia model and miR-126 (Endo-Takahashi et al, 2014), which promotes angiogenesis by canceling the negative regulators of vascular endothelial growth factor (VEGF) signaling (Fish et al, 2008;van Solingen et al, 2009;Wang et al, 2008). Before the in vivo transfection experiments, we confirmed the interaction between miRNA and BLs containing a cationic lipid previously reported effective for the preparation of pDNA loaded-BLs (Endo-Takahashi et al, 2013).…”

Section: Microrna-loaded Blsmentioning

confidence: 99%

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Nonviral Gene Delivery Systems by the Combination of Bubble Liposomes and Ultrasound

Omata

Negishi

Suzuki

et al. 2015

Nonviral Vectors for Gene Therapy - Physical Methods and Medical Translation

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“…Endo-Takahashi et al 14 recently used UTMD to deliver miR-126 to the vasculature of mice with hindlimb ischemia and found a beneficial effect, including an increase in the expression of several angiogenic factors, coupled with improvement in blood flow. However, their study neither assessed the mechanism(s) of the proangiogenic effects of miR-126 nor did they assess the maturity and stability of the formed vessels.…”

Section: 1415mentioning

confidence: 99%