Systemic defense activation by COS-OGA in rice against root-knot nematodes depends on stimulation of the phenylpropanoid pathway

Singh, Richard Raj; Chinnasri, Buncha; Smet, Lien De; Haeck, Ashley; Demeestere, Kristof; Cutsem, Pierre Van; Aubel, Géraldine van; Gheysen, Godelieve; Kyndt, Tina

doi:10.1016/j.plaphy.2019.07.003

Cited by 47 publications

(47 citation statements)

References 37 publications

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“…Indeed, we observed a primed increase in PAL-activity in shoots and roots of AO-sprayed plants upon nematode infection, although only at a later time point. Similarly, our previous research (Singh et al, 2019) showed the importance of the phenylpropanoid pathway in activation of systemic defense against RKN in COS-OGA sprayed rice plants. Which specific phenylpropanoid-derived molecule is important for this systemic signaling remains to be elucidated.…”

Section: Discussionsupporting

confidence: 66%

“…Recently, a novel registered defense elicitor-combining chitosan oligomers (COS) and pectin-derived oligogalacturonides (OGA), called COS-OGA-was shown to control mildew attack in grapes, cucumbers, tomatoes (van Aubel et al, 2014;Van Aubel et al, 2016), and P. infestans in potato (Van Aubel et al, 2018) and very recently a COS fluorinated derivative chitosan-thiadiazoletrifluorobutene (COSSZFB) was shown to control root knot nematode (RKN) Meloidogyne incognita in cucumber seedlings (Fan et al, 2020). In our previous research, this mixture has also been demonstrated to control RKN M. graminicola in rice, through systemic activation of the plant phenylpropanoid pathway (Singh et al, 2019). Additionally, silicon (Zhan et al, 2018) and thiamine (Huang et al, 2016) were shown to activate rice defense against M. graminicola.…”

Section: Introductionmentioning

confidence: 98%

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Ascorbate Oxidase Induces Systemic Resistance in Sugar Beet Against Cyst Nematode Heterodera schachtii

et al. 2020

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Ascorbate oxidase (AO) is an enzyme involved in catalyzing the oxidation of apoplastic ascorbic acid (AA) to dehydroascorbic acid (DHA). In this research, the potential of AO spraying to induce systemic resistance was demonstrated in the interaction between sugar beet root and cyst nematode Heterodera schachtii and the mechanism was elucidated. Plant bioassays showed that roots of AO-sprayed plants were infested by a significantly lower number of females and cysts when compared with mock-sprayed control plants. Hormone measurements showed an elevated level of jasmonic acid (JA) salicylic acid (SA) and ethylene (ET) in the roots of AO-sprayed plants, with a dynamic temporal pattern of activation. Experiments with chemical inhibitors showed that AOinduced systemic resistance is partially dependent on the JA, ET and SA pathways. Biochemical analyses revealed a primed accumulation of hydrogen peroxide (H 2 O 2), and phenylalanine ammonia lyase (PAL) activity in the roots of AO-sprayed plants upon infection by cyst nematodes. In conclusion, our data shows that AO works as an effective systemic defense priming agent in sugar beet against cyst nematode infection, through activation of multiple basal plant defense pathways.

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 98%

Ascorbate Oxidase Induces Systemic Resistance in Sugar Beet Against Cyst Nematode Heterodera schachtii

et al. 2020

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“…As low toxic pesticides, chitosan and COS can be manufactured as sustainable pesticides encapsulating materials [34,35], or modified by grafting functional groups [36]. The methods can be the route to deal with the toxicity of nematicide [7,37]. In this study, the latter route, which we believe more convenient and easier, was chosen.…”

Section: Discussionmentioning

confidence: 99%

Chitosan Oligosaccharide Fluorinated Derivative Control Root-Knot Nematode (Meloidogyne incognita) Disease Based on the Multi-Efficacy Strategy

et al. 2020

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Plant root-knot nematode disease is a great agricultural problem and commercially available nematicides have the disadvantages of high toxicity and limited usage; thus, it is urgent to develop new nematicides derived from nature substances. In this study, a novel fluorinated derivative was synthesized by modifying chitosan oligosaccharide (COS) using the strategy of multiple functions. The derivatives were characterized by FTIR, NMR, elemental analysis, and TG/DTG. The activity assays show that the derivatives can effectively kill the second instar larvae of Meloidogyne incognita in vitro, among them, chitosan-thiadiazole-trifluorobutene (COSSZFB) perform high eggs hatching inhibitory activity. The derivatives can regulate plant growth (photosynthetic pigment), improve immunity (chitinase and β-1,3-glucanase), and show low cytotoxicity and phytotoxicity. According to the multi-functional activity, the derivatives exhibit a good control effect on plant root-knot nematode disease in vivo. The results demonstrate that the COS derivatives (especially fluorinated derivative) perform multiple activities and show the potential to be further evaluated as nematicides.

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“…Metabolic markers could also be used to screen for induced resistance. Resistance inducers are exogenously applied chemicals or microbes which stimulate the plant immune response against pests or pathogens (Heil, 2001;Eyles et al, 2010;Pieterse et al, 2014;Mauch-Mani et al, 2017), including PPN (Oka et al, 1999;Oka and Cohen, 2001;Molinari and Baser, 2010;Vos et al, 2013;Fujimoto et al, 2015;Ji et al, 2015;Huang et al, 2016;Zhan et al, 2018;Singh et al, 2019). If metabolic markers can be reliably correlated to induced resistance in a given plant-PPN system, they can be used to screen for novel resistance inducers and to study the longevity of induced resistance in a more high-throughput manner than possible through conventional inoculation experiments.…”

Section: Possible Applications Of Anps In Ppn Controlmentioning

confidence: 99%

A Phytochemical Perspective on Plant Defense Against Nematodes

et al. 2020

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Systemic defense activation by COS-OGA in rice against root-knot nematodes depends on stimulation of the phenylpropanoid pathway

Cited by 47 publications

References 37 publications

Ascorbate Oxidase Induces Systemic Resistance in Sugar Beet Against Cyst Nematode Heterodera schachtii

Ascorbate Oxidase Induces Systemic Resistance in Sugar Beet Against Cyst Nematode Heterodera schachtii

Chitosan Oligosaccharide Fluorinated Derivative Control Root-Knot Nematode (Meloidogyne incognita) Disease Based on the Multi-Efficacy Strategy

A Phytochemical Perspective on Plant Defense Against Nematodes

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