Systemic corazonin signalling modulates stress responses and metabolism in<i>Drosophila</i>

Kubrak, Olga I.; Lushchak, Oleh; Zandawala, Meet; Nässel, Dick R.

doi:10.1098/rsob.160152

Cited by 97 publications

(126 citation statements)

References 108 publications

(200 reference statements)

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“…By means of Crz peptide injections we also examined the in vivo effects of increased Crz signaling on chill coma tolerance and desiccation survival. Our findings reveal that Crz acts upstream of CAPA signaling to regulate stress-associated physiology, in addition to its direct actions on the fat body that had been demonstrated earlier [9].…”

Section: Introductionsupporting

confidence: 71%

“…Previously, we showed that systemic Crz signaling modulates feeding and nutritional stress through direct actions on the fat body as well as via interactions with AKH and insulinsignaling [9]. Since CrzR is highly expressed in the CNS (Figure S1), we hypothesized that 12 Crz may interact with other peptidergic signaling to maintain physiological homeostasis associated with stress.…”

Section: Crzr Is Expressed In Capa Neuronsmentioning

confidence: 96%

“…In the vinegar fly Drosophila melanogaster, several peptide hormones and neuropeptides have been shown to influence responses to nutrient and osmotic stress via actions on peripheral tissues such as the liver-like fat body and Malpighian (renal) tubules that are analogous to the mammalian kidneys. These hormones include Drosophila insulin-like peptides (DILPs), adipokinetic hormone (AKH), corazonin (Crz), leucokinin, diuretic hormone 44 (DH44) and capability gene-derived CAPA peptides [1][2][3][4][5][6][7][8][9][10][11][12]. In mammals, the hypothalamic-pituitary-adrenal (HPA) axis coordinates stress responses via the sequential secretion of corticotropinreleasing factor (CRF), adrenocorticotropic hormone (ACTH) and cortisol [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Previous work in Drosophila has shown that Crzproducing dorsolateral peptidergic neurons (DLPs) affect metabolism, resistance to various stresses and stress-induced locomotor activity [1,23]. Moreover, knockdown of the Crz receptor, CrzR, in the fat body and salivary glands also influences survival under stress, as well as feeding and insulin signaling [9]. Thus, several effects observed following Crz knockdown in the central nervous system (CNS) could be recapitulated by knocking down 4 the CrzR in the fat body and salivary glands.…”

Section: Introductionmentioning

confidence: 99%

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A neuroendocrine pathway modulating osmotic stress inDrosophila

Zandawala

Nguyen

Segura

et al. 2019

Preprint

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Environmental factors challenge the physiological homeostasis in animals, thereby evoking stress responses. Various mechanisms have evolved to counter stress at the organism level, including regulation by neuropeptides. Corazonin (Crz) is a neuropeptide known to regulate stress-associated physiology in Drosophila. However, the neural circuits and hormonal pathways underlying this regulation are poorly known. To unveil targets of Crz, we mapped the Crz receptor (CrzR) expression in Drosophila central nervous system (CNS).The CrzR is expressed in peptidergic neurons in the adult CNS, including the median neurosecretory cells and certain clock neurons in the brain, Hugin neurons in the subesophageal zone (SEZ) and CAPA-expressing neurosecretory cells in the SEZ and abdominal neuromeres (Va neurons). We focused on the Va neurons since they produce osmoregulatory peptides (CAPA-1 and CAPA-2), which mediate recovery from desiccation and cold stress. Trans-Tango labeling to determine synaptic partners failed to reveal connections between Crz and Va neurons, suggesting hormonal interactions. To validate the signaling between Crz and Va neurons, we show that knockdown of Crz in Crzproducing neurons and CrzR in Va neurons increases survival under desiccation and delays chill coma recovery. Moreover, immunolabeling data suggests that Crz is released under nutritional and osmotic stress, and in vivo Crz peptide injections influence responses to desiccation and chill coma. Thus, Crz modulates Va neurons to maintain osmotic/ionic homeostasis, which in turn influences stress tolerance. Taken together with previous data, we propose that Crz acts via both the fat body and peptidergic neurons in the CNS to regulate stress-associated physiology.

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Section: Introductionsupporting

confidence: 71%

Section: Crzr Is Expressed In Capa Neuronsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A neuroendocrine pathway modulating osmotic stress inDrosophila

Zandawala

Nguyen

Segura

et al. 2019

Preprint

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“…4A, right). Based on these observations and on the fact that corazonin can act systemically in Drosophila (Kubrak et al, 2016), we hypothesized that the increased levels of corazonin in Harpegnathos workers could affect vitellogenin expression in the fat body, potentially linking the behavioral and reproductive changes observed during the worker–gamergate transition. Indeed, injections of corazonin peptide into the gasters of transitioning ants inhibited vitellogenin expression in the fat body (Fig.…”

Section: Resultsmentioning

confidence: 99%

The Neuropeptide Corazonin Controls Social Behavior and Caste Identity in Ants

Gospočić

Shields

Glastad

et al. 2017

Cell

145

179

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SUMMARY Social insects are emerging models to study how gene regulation affects behavior because their colonies comprise individuals with the same genomes but greatly different behavioral repertoires. To investigate the molecular mechanisms that activate distinct behaviors in different castes, we exploited a natural behavioral plasticity in Harpegnathos saltator, where adult workers can transition to a reproductive, queen-like state called gamergate. Analysis of brain transcriptomes during the transition revealed that corazonin, a neuropeptide homologous to the vertebrate gonadotropin-releasing hormone, was downregulated as workers became gamergates. Corazonin was also preferentially expressed in workers and/or foragers from other social insect species. Injection of corazonin in transitioning Harpegnathos individuals suppressed expression of vitellogenin in the brain and stimulated worker-like hunting behaviors, while inhibiting gamergate behaviors such as dueling and egg deposition. We propose that corazonin is a central regulator of caste identity and behavior in social insects.

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A journey into the world of insect lipid metabolism

Toprak¹,

Hegedus

Doğan³

et al. 2020

Arch Insect Biochem Physiol

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Lipid metabolism is fundamental to life. In insects, it is critical, during reproduction, flight, starvation, and diapause. The coordination center for insect lipid metabolism is the fat body, which is analogous to the vertebrate adipose tissue and liver. Fat body contains various different cell types; however, adipocytes and oenocytes are the primary cells related to lipid metabolism. Lipid metabolism starts with the hydrolysis of dietary lipids, absorption of lipid monomers, followed by lipid transport from midgut to the fat body, lipogenesis or lipolysis in the fat body, and lipid transport from fat body to other sites demanding energy. Lipid metabolism is under the control of hormones, transcription factors, secondary messengers and posttranscriptional modifications. Primarily, lipogenesis is under the control of insulin‐like peptides that activate lipogenic transcription factors, such as sterol regulatory element‐binding proteins, whereas lipolysis is coordinated by the adipokinetic hormone that activates lipolytic transcription factors, such as forkhead box class O and cAMP‐response element‐binding protein. Calcium is the primary–secondary messenger affecting lipid metabolism and has different outcomes depending on the site of lipogenesis or lipolysis. Phosphorylation is central to lipid metabolism and multiple phosphorylases are involved in lipid accumulation or hydrolysis. Although most of the knowledge of insect lipid metabolism comes from the studies on the model Drosophila; other insects, in particular those with obligatory or facultative diapause, also have great potential to study lipid metabolism. The use of these models would significantly improve our knowledge of insect lipid metabolism.

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Systemic corazonin signalling modulates stress responses and metabolism inDrosophila

Cited by 97 publications

References 108 publications

A neuroendocrine pathway modulating osmotic stress inDrosophila

A neuroendocrine pathway modulating osmotic stress inDrosophila

The Neuropeptide Corazonin Controls Social Behavior and Caste Identity in Ants

A journey into the world of insect lipid metabolism

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