Systemic co-administration of depsipeptide selectively targets transfection enhancement to specific tissues and cell types

Meme Kanserinde Epigenetik Tedavi

“…Depsipeptid metastatik meme kanseri hücrelerinde insan P53 geninin ifadesini artırırken ancak tümörlü olmayan komşu hücrelerinde etkisi yoktur 90 . 4 saatlik beklemeden sonra güvenli bir şekilde tatbik edilebilir 91 .…”

“…Sistin olmayan bir disülfid köprüsü içeren Chromobacterium violaceum'dan izole edilmiş bir bisiklik peptidtir ve kültüre edilmiş tümör hücrelerinde lipoplex türevi genlerin ifadesini arttırır. Depsipeptid metastatik meme kanseri hücrelerinde insan P53 geninin ifadesini artırırken ancak tümörlü olmayan komşu hücrelerinde etkisi yoktur 90 . 4 saatlik beklemeden sonra güvenli bir şekilde tatbik edilebilir 91 .…”

Small molecules convey big messages: Boosting non-viral nucleic acid delivery with low molecular weight drugs

“…In addition, this improvement was blocked by treatment with free haloperidol and no differences in transgene expression were obtained for targeted and non-targeted LPS in CHO cells, which express low sigma receptor levels [46]. Subsequently, the group of Leaf Huang proposed the use of anisamide as a sigma-1 receptor ligand [47]. Incorporation of anisamide into their lipid-based carrier improved siRNA delivery by 4-fold in B16F10 murine melanoma cells.…”

“…From this point onwards, the transgene expression is furthermore subjected to epigenetic regulation mechanisms [74]. Histone hyperacetylation is typically associated with gene expression by facilitating access of transcription factors and RNA polymerase to the unwound DNA [47,75]. In contrast, histone deacetylation and methylation of a pDNA promoter region silences transgene expression [76].…”

Epigenetic Therapy for Breast Cancer

“…Depsipeptide increases the expression of the human p53 gene in metastatic breast cancer cells, but not in adjacent normal cells [59]. It can be safely administered when given as a 4-hour infusion [60].…”