Systemic chemical characterization of Lemna minor by UHPLC‐Q‐Exactive Orbitrap MS coupled with parallel reaction monitoring

Abstract

Lemna minor L. (LM) has been used for measles opacity, rubella itching, edema, and oliguria, and the main active ingredients were flavonoids, namely, apigenin, apigenin‐7‐O‐glucoside, and luteolin‐7‐O‐glucoside. However, few systematic analyses of their constituents have been performed; thus, it was necessary to establish a fast and efficient method to identify the chemical composition of LM. In this study, the UHPLC‐Q‐Exactive Orbitrap mass spectrometry coupled with parallel reaction monitoring was established. Finally, a total of 112 constituents, including 30 dipeptides, 28 nucleosides, 11 amino acids, 10 organic acids, 10 flavonoids, and 23 other compounds, were identified by MS, diagnostic fragment ions, and retention time. One hundred one of those chemicals were first found in LM, which was very beneficial for the further development and utilization of nutriments and the medicinal use of LM.

Characterization of Bioactive Metabolites and Antioxidant Activities in Solid and Liquid Fractions of Fresh Duckweed (Wolffia globosa) Subjected to Different Cell Wall Rupture Methods

“…Compound 32 was observed in the ESI – and ESI + modes. Previously these compounds were reported in common duckweed, L. minor , 71 L. minor L., 72 and Landoltia punctata . 73 Chen et al 70 reported that apigenin 7-glucoside has been shown in vitro anti-inflammatory activities.…”

“…minor, L. minor L., and Landoltia punctata . Chen et al reported that apigenin 7-glucoside has been shown in vitro anti-inflammatory activities.…”