Systemic and mucosal immune response of rainbow trout to immunization with an attenuated Flavobacterium psychrophilum vaccine strain by different routes

Makesh, M.; Sudheesh, Ponnerassery S.; Cain, Kenneth D.

doi:10.1016/j.fsi.2015.02.003

Cited by 76 publications

(48 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…columnare , with a similar pattern of increase in the expression of IgM , IgD and IgZ genes as that observed in the present study (Tian et al, 2009). Recently, Makesh et al (2015) examined IgM antibody levels, and IgM , IgD and IgT gene expression in the systemic and mucosal immune systems of rainbow trout following immunization with attenuated F . psychrophilum , and found an elevated level of IgM antibodies, and of IgM , IgD and IgT gene expression.…”

Section: Discussionsupporting

confidence: 87%

Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella

Luo

Liu

et al. 2016

Chin. J. Ocean. Limnol.

View full text Add to dashboard Cite

Flavobacterium columnare causes columnaris disease in freshwater fi sh. In the present study, the antigenic regions of fi ve outer membrane proteins (OMPs), including zinc metalloprotease, prolyl oligopeptidase, thermolysin, collagenase and chondroitin AC lyase, were bioinformatically analyzed, fused together, and then expressed as a recombinant fusion protein in Escherichia coli . The expressed protein of 95.6 kDa, as estimated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was consistent with the molecular weight deduced from the amino acid sequence. The purifi ed recombinant protein was used to vaccinate the grass carp, Ctenopharyngodon idella . Following vaccination of the fi sh their IgM antibody levels were examined, as was the expression of IgM , IgD and IgZ immunoglobulin genes and other genes such as MHC Iα and MHC I I β , which are also involved in adaptive immunity. Interleukin genes ( IL ), including IL -1β , IL -8 and IL -10 , and type I and type II interferon ( IFN ) genes were also examined. At 3 and 4 weeks post-vaccination (wpv), signifi cant increases in IgM antibody levels were observed in the fi sh vaccinated with the recombinant fusion protein, and an increase in the expression levels of IgM , IgD and IgZ genes was also detected following the vaccinations, thus indicating that an adaptive immune response was induced by the vaccinations. Early increases in the expression levels of IL and IFN genes were also observed in the vaccinated fi sh. At four wpv, the fi sh were challenged with F . column a re , and the vaccinated fi sh showed a good level of protection against this pathogen, with 39% relative percent survival (RPS) compared with the control group. It can be concluded, therefore, that the fi ve OMPs, in the form of a recombinant fusion protein vaccine, induced an immune response in fi sh and protection against F . columnare .

show abstract

Section: Discussionsupporting

confidence: 87%

Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella

Luo

Liu

et al. 2016

Chin. J. Ocean. Limnol.

View full text Add to dashboard Cite

show abstract

“…In one Fp challenge study in rainbow trout, using intraperitoneal injection (but not immersion) with killed bacteria, a serum IgM response was induced at week 6, while mucus IgM peaked at week 9 (LaFrentz et al, 2009). In a different study, when rainbow trout were immunized intraperitoneally with attenuated Fp strain CSF259-93-B, the levels of IgM increased in serum, gill, and skin mucus by day 28 post-challenge (Makesh et al, 2015). …”

Section: Discussionmentioning

confidence: 99%

“…A few gene-expression studies have reported on the roles of IgT in response to bacterial pathogens in recent years. In one study, a protective role for IgT against Fp was suggested because secreted heavy chain tau1 (HCtau1) levels increased in the gills following immersion or anal intubation with Fp (Makesh et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

A BCWD-resistant line of rainbow trout exhibits higher abundance of IgT+ B cells and heavy chain tau transcripts compared to a susceptible line following challenge with Flavobacterium psychrophilum

Zwollo

Hennessey

Moore

et al. 2017

Developmental & Comparative Immunology

View full text Add to dashboard Cite

Bacterial Cold Water Disease (BCWD) is a common, chronic disease in rainbow trout, and is caused by the gram-negative bacterium Flavobacterium psychrophilum (Fp). Through selective breeding, the National Center for Cool and Cold Water Aquaculture has generated a genetic line that is highly resistant to Fp challenge, designated ARS-Fp-R (or R-line), as well as a susceptible “control” line, ARS-Fp-S (S-line). In previous studies, resistance to Fp had been shown to correlate with naive animal spleen size, and further, naïve R-line trout had been shown to have a lower abundance of IgM+ and IgM++ cells compared to S-line fish. Here we wished to first determine whether the abundance of IgT+ and/or IgT++ cells differed between the two lines in naïve fish, and if so, how these patterns differed after in vivo challenge with Fp. Fp challenge was by intramuscular injection of live Fp and tissue collections were on days 5, 6, and/or 28 post-challenge, in two independent challenge experiments. Flow cytometric and gene expression analyses revealed that naïve R-line fish had a higher abundance of IgT+ B cells in their anterior kidney, spleen, and blood, compared to S line fish. Further, that after Fp challenge, this difference was maintained between the two lines. Lastly, abundance of IgT+ B cells and expression of secHCtau correlated with lower Fp pathogen loads in challenged fish. In the anterior kidney, IgM+ B cell abundance correlated with increased Fp loads. Together, these results suggest that IgT+ B lineage cells may have a protective function in the immune response to Fp.

show abstract

“…However, the same study showed that oral vaccination against infectious pancreatic necrosis virus (IPNV) induced an increase in both IgT + and IgM + cells, indicating that both cell types are important for mucosal responses. It is widely accepted that IgM expression is dominant in absolute terms in all organs (8–12) and is essential for immune protection against different pathogens upon different routes of infection (1, 9). IgT, despite being generally less abundant than IgM in number of transcripts and cells, is undeniably crucial in mucosal immune responses, but its role in systemic responses (13, 14) and the role of IgM in mucosal responses should not be discarded.…”

Section: Introductionmentioning

confidence: 99%

Differential Modulation of IgT and IgM upon Parasitic, Bacterial, Viral, and Dietary Challenges in a Perciform Fish

et al. 2016

View full text Add to dashboard Cite

Three different immunoglobulin (Ig) isotypes can be found in teleost fish, IgM, IgD, and the teleost-specific IgT. IgM is considered to have a systemic activity, and IgT is attributed a mucosal role, similar to mammalian IgA. In this study, the complete sequence of gilthead sea bream IgM and IgT in their membrane (m) and soluble (s) forms are described for the first time in a perciform fish. Their constitutive gene expression is analyzed in different tissues, and their regulation upon viral, bacterial, parasitic, mucosal vaccination and dietary challenges are studied. GCB IgM and IgT have the prototypical structure when compared to other fish Igs. The constitutive expression of sIgM was the highest overall in all tissues, whereas mIgT expression was highest in mucosal tissues, such as gills and intestine. IgM and IgT were differentially regulated upon infection. IgT was highly upregulated locally upon infection with the intestinal parasite Enteromyxum leei or systemically after Nodavirus infection. Long-term intestinal parasitic infections increased the serum titer of both isotypes. Mucosal vaccination against Photobacterium damselae subsp. piscicida finely regulated the Ig response inducing a systemic increase of IgM titers in serum and a local IgT response in skin mucus when animals were exposed to the pathogen by bath challenge. Interestingly, plant-based diets inhibit IgT upregulation upon intestinal parasitic challenge, which was related to a worse disease outcome. All these results corroborate the mucosal role of IgT and emphasize the importance of a finely tuned regulation of Ig isotypes upon infection, which could be of special interest in vaccination studies.

show abstract

Systemic and mucosal immune response of rainbow trout to immunization with an attenuated Flavobacterium psychrophilum vaccine strain by different routes

Cited by 76 publications

References 52 publications

Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella

Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella

A BCWD-resistant line of rainbow trout exhibits higher abundance of IgT+ B cells and heavy chain tau transcripts compared to a susceptible line following challenge with Flavobacterium psychrophilum

Differential Modulation of IgT and IgM upon Parasitic, Bacterial, Viral, and Dietary Challenges in a Perciform Fish

Contact Info

Product

Resources

About