Systemic Acquired Silencing of a Gusa Transgene in Apple

Hättasch, C.; Flachowsky, Henryk; Hanke, Magda‐Viola; Szankowski, I.; Wolff, Philip; Waidmann, Sascha

doi:10.17660/actahortic.2009.839.51

Cited by 1 publication

(1 citation statement)

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“…To generate artificial dsRNAs through transgenes, constructs have been designed to express small hairpin RNA (hpRNA) (Hily et al ., ; Kusaba, ). The hpRNA‐mediated RNAi in plants has been shown to engender long‐distance silencing signals and acts through the viral defense pathway in plants (Fusaro et al ., ; Hättasch et al ., ; Ryabov et al ., ; Tournier et al ., ; Yaegashi et al ., ). As the first stage of a proof‐of‐concept study to determine whether transformed rootstocks can be used to obtain viral resistance in scion cultivars, we report here on the transformation of the hybrid cherry rootstocks ‘Gisela 6’ (tolerant to PNRSV) and ‘Gisela 7’ (sensitive to PNRSV) with two RNAi vectors pTRAP6i and pART27–PNRSV, each containing inverted repeat (IR) regions of a PNRSV sequence (Figure a).…”

Section: Introductionmentioning

confidence: 97%

Engineering cherry rootstocks with resistance to Prunus necrotic ring spot virus through RNAi‐mediated silencing

Song

Sink

Walworth

et al. 2013

Plant Biotechnology Journal

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Summary Prunus necrotic ringspot virus (PNRSV) is a major pollen‐disseminated ilarvirus that adversely affects many Prunus species. In this study, an RNA interference (RNAi) vector pART27–PNRSV containing an inverted repeat (IR) region of PNRSV was transformed into two hybrid (triploid) cherry rootstocks, ‘Gisela 6’ (GI 148‐1) and ‘Gisela 7’(GI 148‐8)’, which are tolerant and sensitive, respectively, to PNRSV infection. One year after inoculation with PNRSV plus Prune Dwarf Virus, nontransgenic ‘Gisela 6’ exhibited no symptoms but a significant PNRSV titre, while the transgenic ‘Gisela 6’ had no symptoms and minimal PNRSV titre. The nontransgenic ‘Gisela 7’ trees died, while the transgenic ‘Gisela 7’ trees survived. These results demonstrate the RNAi strategy is useful for developing viral resistance in fruit rootstocks, and such transgenic rootstocks may have potential to enhance production of standard, nongenetically modified fruit varieties while avoiding concerns about transgene flow and exogenous protein production that are inherent for transformed fruiting genotypes.

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