“…Despite their simplicity, these cysts have previously been used as a suitable model system to study the establishment of cell polarity and cell junctions (Bryant & Mostov, 2008;Dukes et al, 2011;Martín-Belmonte et al, 2008;Vidal-Quadras et al, 2017;Yonemura, 2014), epithelial morphogenesis and physiology (Alfonso-Pérez et al, 2022;Guo et al, 2008;Imai et al, 2015;O'Brien et al, 2002;Wells et al, 2013;Yu et al, 2007), tumor progression (Fessenden et al, 2018;Sakurai et al, 2012;Schmeichel & Bissell, 2003;L. Yan et al, 2021), and for exploring the constraints on epithelial tissue morphogenesis (Beck et al, 2022). MDCK cysts have provided a lot of useful information to study more complex systems helping to understand the selforganization in organoids, embryoids and the early stages of mammal development (Beck et al, 2022;Carleton et al, 2022;Metzger et al, 2018).…”