Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteria

Abstract

Bacterial genome editing commonly relies on chromosomal cleavage with Cas nucleases to counter-select against unedited cells. However, editing normally requires efficient recombination and high transformation efficiencies, which are unavailable in most strains. Here, we show that systematically attenuating DNA targeting activity enables RecA-mediated repair in different bacteria, allowing chromosomal cleavage to drive genome editing. Attenuation can be achieved by altering the format or expression strength of guide (g)RNAs; using nucleases with reduced cleavage activity; or engineering attenuated gRNAs (atgRNAs) with disruptive hairpins, perturbed nuclease scaffolds, non-canonical PAMs, or guide mismatches. These modifications greatly increase cell counts and even improve the efficiency of different types of edits for Cas9 and Cas12a in Escherichia coli and Klebsiella oxytoca. We further applied atgRNAs to restore ampicillin sensitivity in Klebsiella pneumoniae, establishing a new resistance marker for genetic studies. Attenuating DNA targeting thus offers a counterintuitive means to achieve CRISPR-driven editing across bacteria.

CRISPR/Cas9-Mediated Multiplexed Genome Editing in Aspergillus oryzae

“…Furthermore, efficient HR-based gene editing requires a host with high HR efficiency and transformation efficiency [ 17 ]. Chase L. Beisel proposed and verified that the weakened CRISPR-Cas system could obtain more transformants during the transformation process, and improve genome editing efficiency to a certain extent [ 18 ].…”