Systematic survey of the function of ROP regulators and effectors during tip growth in the moss <i>Physcomitrella patens</i>

Bascom, Carlisle; Burkart, Graham M.; Mallett, Darren R; O’Sullivan, Jacquelyn E; Tomaszewski, Alexis; Walsh, Katherine; Bezanilla, Magdalena

doi:10.1093/jxb/ery376

Cited by 26 publications

(16 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In plants, ROP GTPase, also called RAC-GTPase, plays a fundamental role in several important cellular processes, such as tip growth of pollen tubes and root hairs, regulation of the actin cytoskeleton, and hormone and stress response [107,145,146,147]. Similar to the Rho family proteins in fungi and mammalian cells, ROP GTPases regulate plant actin and MT cytoskeletal organization and dynamics [51,59,107,148,149,150].…”

Section: The Actin Cytoskeleton Acts a Potential Downstream Of Calmentioning

confidence: 99%

Actin Cytoskeleton as Actor in Upstream and Downstream of Calcium Signaling in Plant Cells

Qian

Xiang

2019

IJMS

View full text Add to dashboard Cite

In plant cells, calcium (Ca2+) serves as a versatile intracellular messenger, participating in several fundamental and important biological processes. Recent studies have shown that the actin cytoskeleton is not only an upstream regulator of Ca2+ signaling, but also a downstream regulator. Ca2+ has been shown to regulates actin dynamics and rearrangements via different mechanisms in plants, and on this basis, the upstream signaling encoded within the Ca2+ transient can be decoded. Moreover, actin dynamics have also been proposed to act as an upstream of Ca2+, adjust Ca2+ oscillations, and establish cytosolic Ca2+ ([Ca2+]cyt) gradients in plant cells. In the current review, we focus on the advances in uncovering the relationship between the actin cytoskeleton and calcium in plant cells and summarize our current understanding of this relationship.

show abstract

Section: The Actin Cytoskeleton Acts a Potential Downstream Of Calmentioning

confidence: 99%

Actin Cytoskeleton as Actor in Upstream and Downstream of Calcium Signaling in Plant Cells

Qian

Xiang

2019

IJMS

View full text Add to dashboard Cite

show abstract

“…RNAi has been extensively employed in the model moss P. patens for both discovery and validation of gene function (Vidali et al, 2007;Augustine et al, 2008;Prigge et al, 2010;Vidali et al, 2010;Augustine et al, 2011;Wu et al, 2011;Miki et al, 2015;Bascom et al, 2019). We attribute the popularity of dsRNA-based RNAi in P. patens to a method that uses an internal fluorescent reporter of RNAi to obtain results in one week (Bezanilla et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Robust Survival-Based RNA Interference of Gene Families Using in Tandem Silencing of Adenine Phosphoribosyltransferase

et al. 2020

View full text Add to dashboard Cite

show abstract

“…LR clonase (Invitrogen) reactions were used to transfer these coding sequences into yeast 2-hybrid vectors. Sec23D was transferred to pGBKT7-Gate (Bascom Jr et al, 2018), and all Sec24 genes were transferred to pGADT7-Gate (Bascom Jr et al, 2018).…”

Section: Generating the Sec23mentioning

confidence: 99%

COPII Sec23 proteins form isoform-specific ER exit sites with differential effects on polarized growth

Chang

Ryken

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

30COPII, a coat of proteins that form vesicles on the ER, mediates vesicle 31 traffic from the ER to the Golgi. In contrast to metazoans that have few genes 32 encoding each COPII component, plants have expanded these gene families 33 leading to the hypothesis that plant COPII has functionally diversified. Here, we 34 analyzed the gene families encoding for the Sec23/24 heterodimer in the moss 35 Physcomitrium (Physcomitrella) patens. In P. patens, Sec23 and Sec24 gene 36 families are each comprised of seven genes. We discovered that Sec23D is 37 functionally redundant with Sec23E and together they are essential for 38 protonemal development. Sec23D is critical for secretion to the Golgi and the 39 plasma membrane in tip growing protonemata. Sec23D preferentially associates 40 with Sec24C/D, also essential for protonemata. In contrast, the remaining five 41

show abstract

Systematic survey of the function of ROP regulators and effectors during tip growth in the moss Physcomitrella patens

Abstract: Systematic loss of function studies reveal the influence that each family of Rho of plants (ROP) regulators has on tip growth in the moss, Physcomitrella patens.

Cited by 26 publications

References 35 publications

Actin Cytoskeleton as Actor in Upstream and Downstream of Calcium Signaling in Plant Cells

Actin Cytoskeleton as Actor in Upstream and Downstream of Calcium Signaling in Plant Cells

Robust Survival-Based RNA Interference of Gene Families Using in Tandem Silencing of Adenine Phosphoribosyltransferase

COPII Sec23 proteins form isoform-specific ER exit sites with differential effects on polarized growth

Contact Info

Product

Resources

About