Systematic Study of Preimplantation Stages of Pregnancy in the Sheep

Bm, Bindon

doi:10.1071/bi9710131

Cited by 45 publications

(32 citation statements)

References 15 publications

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“…Both the uterine and glandular epithelial cell heights show significant linear increases with increasing dose of progesterone. It is significant that, with 16 mg progesterone, both these parameters reach the magnitude of that in ewes in normal pregnancy (Bindon 1971 .....…”

Section: (A) Experiments 1: Progesterone Requirements For Implantationmentioning

confidence: 99%

“…The sections were stained with haematoxylin and eosin. Histological measurements were made as previously described (Bindon 1971).…”

Section: (D) Examination Of Reproductive Tractsmentioning

confidence: 99%

“…The purpose of the present study was to examine the progesterone requirements for implantation in the ewe especially with regard to the time of involvement in relation to the preimplantation phenomena described in an earlier paper (Bindon 1971). This was approached first by performing ovariectomy at various times and studying the dosage requirements of progesterone for embryo survival.…”

Section: Introductionmentioning

confidence: 99%

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The Role of Progesterone in Implantation in the Sheep

Bm¹

1971

Aust. Jnl. Of Bio. Sci.

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SummaryMerino ewes were ovariectomized on days 4, 8, or 12 of pregnancy and injected daily from that time with 1, 4, or 16 mg progesterone in oil. The ewes were killed on day 20 and examined for embryos. There was a clear effect of dose of progesterone on the number and viability of embryos. Of the ewes receiving 1, 4, and 16 mg progesterone there were 0 out of 12, 2 out of 11, and 11 out of 14 with viable embryos on day 20. The effect of increasing progesterone was also seen in significantly increased glandular and luminal epithelial cell heights in ewes receiving 16 mg per day.Plasma progesterone was measured daily during days 1-5 and twice daily during days 6-15 of pregnancy and of the oestrous cycle. The numbers of corpora lutea and embryos in these animals were verified at laparotomy on days 15-18. The difference between pregnant and non-pregnant ewes with regard to plasma progesterone was not significant until days 16-17. Both pregnant and non-pregnant ewes had highest levels of progesterone during the period day 10 to day 14. Different ewes had peak values at different times so that the mean progesterone values took the form of a variable plateau, lasting several days, of 2-3 ng/ml.Peak levels of progesterone appeared in the plasma at times consistent with the idea that this hormone is responsible for the rapid preimplantation growth phase of the embryo during days 11-15.

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Section: (A) Experiments 1: Progesterone Requirements For Implantationmentioning

confidence: 99%

“…The sections were stained with haematoxylin and eosin. Histological measurements were made as previously described (Bindon 1971).…”

Section: (D) Examination Of Reproductive Tractsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Role of Progesterone in Implantation in the Sheep

Bm¹

1971

Aust. Jnl. Of Bio. Sci.

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“…Whereas in the mouse the blastocyst hatches from the zona pellucida and implants on the uterine wall on the fourth day of pregnancy (Finn and McLaren, 1967;Orsini and McLaren, 1967), in the sheep hatching and implantation occur about eight days apart (Bindon, 1971;Rowson and Moor, 1976 (fig 3a, b) showed the highest anti-LIF titres 6 days after the tertiary injection, on day 16 (fig 3a) and day 22 (fig 3b), respectively. By day 22 (fig 3a) and day 35 (fig 3b) Parr and Parr ( 1986) demonstrated that IgG in mouse serum is taken up by uterine luminal and glandular epithelial cells (Wang et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

“…The sheep, unlike the human and mouse, has a characteristically long intrauterine blastocyst development before implantation (Bindon, 1971). Whereas in the mouse the blastocyst hatches from the zona pellucida and implants on the uterine wall on the fourth day of pregnancy (Finn and McLaren, 1967;Orsini and McLaren, 1967), in the sheep hatching and implantation occur about eight days apart (Bindon, 1971;Rowson and Moor, 1976 (fig 3a, b) showed the highest anti-LIF titres 6 days after the tertiary injection, on day 16 (fig 3a) and day 22 (fig 3b), respectively.…”

Section: Introductionmentioning

confidence: 99%

Effect of immunisation against leukaemia inhibitory factor on the establishment of pregnancy in sheep

Vogiagis¹,

Salamonsen²,

Sandeman³

et al. 1997

Reprod. Nutr. Dev.

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The early development of the sheep trophoblast and the involvement of cell death

Carnegie

McCully

Robertson³

1985

Am. J. Anat.

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During the period of rapid elongation prior to the initiation of placental attachment (days 12-16 of gestation), the ovine blastocyst consists of a single layer (primarily) of roughly cuboidal trophoblastic cells with an inner lining of flattened endodermal cells. Well-developed spot desmosomes link the adjacent cell borders in both the trophoblastic and endodermal layers. The trophoblastic cells contain acid phosphatase-positive, lysosomelike organelles, the mean diameter of which increases greatly between days 12 and 16 and whose contents vary during development. Also during the developmental period studied, trophoblast cells accumulate lipid; and periodic acid-Schiff-positive binucleate cells appear within the trophoblast layer. A consistent observation throughout the 5 days of rapid growth and differentiation of the blastocyst was the death and disintegration of some trophoblast cells. These disintegrating cells are usually singly dispersed within the trophoblast, although occasionally groups of four or five are observed. The cell death may indicate overall remodelling of the blastocyst, or the cells may represent genetically deficient cells which are unable to respond to the appropriate signal to differentiate.

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Systematic Study of Preimplantation Stages of Pregnancy in the Sheep

Cited by 45 publications

References 15 publications

The Role of Progesterone in Implantation in the Sheep

The Role of Progesterone in Implantation in the Sheep

Effect of immunisation against leukaemia inhibitory factor on the establishment of pregnancy in sheep

The early development of the sheep trophoblast and the involvement of cell death

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