Systematic review with meta-analysis:<i>Lactobacillus rhamnosus</i>GG in the prevention of antibiotic-associated diarrhoea in children and adults

Szajewska, Hania; Kołodziej, Maciej

doi:10.1111/apt.13404

Cited by 166 publications

(122 citation statements)

References 27 publications

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“…Using BAGEL3, we found that the ORF of the prebacteriocin in L. rhamnosus L156.4 showed 100% identity with that of L. rhamnosus GG (ATCC 53103), a probiotic strain (Szajewska and Kołodziej, 2015) commercialized by Chr. Hansen (Hørsholm, Denmark).…”

Section: Discussionmentioning

confidence: 99%

“…Bacteriocinogenic L. rhamnosus strains have been isolated from human feces (Gorbach, 1996; Gill et al, 2000; Cukrowska et al, 2009; Dimitrijević et al, 2009; Aguilar-Uscanga et al, 2013), vaginal microbiota (Li et al, 2005), fermented beverages (Todorov and Dicks, 2005), grape peels (Sarika et al, 2010), milk samples (Srinivasan et al, 2013), and cheese (Jeong and Moon, 2015). This species is generally recognized as safe (GRAS) and has been widely explored as a probiotic in animal production (Weese and Anderson, 2002) human health (Gill et al, 2000; Cukrowska et al, 2009; Douillard et al, 2013; Szajewska and Kołodziej, 2015) and as a biopreservative in food systems (Cotter et al, 2005; Douillard et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

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In silico Prediction, in vitro Antibacterial Spectrum, and Physicochemical Properties of a Putative Bacteriocin Produced by Lactobacillus rhamnosus Strain L156.4

Oliveira

Silveira

Monteiro³

et al. 2017

Front. Microbiol.

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A bacteriocinogenic Lactobacillus rhamnosus L156.4 strain isolated from the feces of NIH mice was identified by 16S rRNA gene sequencing and MALDI-TOF mass spectrometry. The entire genome was sequenced using Illumina, annotated in the PGAAP, and RAST servers, and deposited. Conserved genes associated with bacteriocin synthesis were predicted using BAGEL3, leading to the identification of an open reading frame (ORF) that shows homology with the L. rhamnosus GG (ATCC 53103) prebacteriocin gene. The encoded protein contains a conserved protein motif associated a structural gene of the Enterocin A superfamily. We found ORFs related to the prebacteriocin, immunity protein, ABC transporter proteins, and regulatory genes with 100% identity to those of L. rhamnosus HN001. In this study, we provide evidence of a putative bacteriocin produced by L. rhamnosus L156.4 that was further confirmed by in vitro assays. The antibacterial activity of the substances produced by this strain was evaluated using the deferred agar-spot and spot-on-the lawn assays, and a wide antimicrobial activity spectrum against human and foodborne pathogens was observed. The physicochemical characterization of the putative bacteriocin indicated that it was sensitive to proteolytic enzymes, heat stable and maintained its antibacterial activity in a pH ranging from 3 to 9. The activity against Lactobacillus fermentum, which was used as an indicator strain, was detected during bacterial logarithmic growth phase, and a positive correlation was confirmed between bacterial growth and production of the putative bacteriocin. After a partial purification from cell-free supernatant by salt precipitation, the putative bacteriocin migrated as a diffuse band of approximately 1.0–3.0 kDa by SDS-PAGE. Additional studies are being conducted to explore its use in the food industry for controlling bacterial growth and for probiotic applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In silico Prediction, in vitro Antibacterial Spectrum, and Physicochemical Properties of a Putative Bacteriocin Produced by Lactobacillus rhamnosus Strain L156.4

Oliveira

Silveira

Monteiro³

et al. 2017

Front. Microbiol.

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“…A 2015 systematic review with a meta-analysis of 10 RCTs involving 1,058 participants found that treatment with LGG compared with placebo or no additional treatment reduced the risk of antibiotic-associated diarrhea (RR 0.44, 95% CI 0.26-0.74, NNT 6, 95% CI 5-14; low quality of evidence), both in children (5 RCTs, n = 445, RR 0.48, 95% CI 0.26-0.89; moderate quality of evidence) and in adults (5 RCTs, n = 613, RR 0.38, 95% CI 0.15-0.92; low quality of evidence) [17]. …”

Section: Bacterial Probioticsmentioning

confidence: 99%

Probiotic Bacterial and Fungal Strains: Claims with Evidence

Szajewska

Konarska²,

Kołodziej³

2016

Dig Dis

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Background: Probiotics are live microorganisms that, when administered in adequate amounts, confer a health benefit for the host. This review summarizes current (i.e. published in the last 5 years) key evidence on probiotic efficacy and its safety in adults and children. To identify relevant data, searches of MEDLINE and the Cochrane Library databases were performed in August 2015 to locate randomized controlled trials (RCTs) or their meta-analyses. The focus was on commonly used, well-specified, bacterial and yeast probiotics for the treatment of gastrointestinal disorders. The MEDLINE database was also searched for evidence-based clinical practice guidelines, developed by scientific societies in the same timeframe. Data on safety of probiotics were obtained from a document developed by the US Agency for Healthcare Research and Quality. Key Messages: A number of relevant RCTs and meta-analyses are available. Saccharomyces boulardii is the most studied yeast probiotic, and Lactobacillus rhamnosus GG is the most studied bacterial probiotic. For both, the best documented fact is their efficacy for the treatment of acute gastroenteritis, especially in children, and for the prevention of antibiotic-associated diarrhea, both in adults and children. There is some evidence to support the use of probiotics to prevent or treat other diseases, such as necrotizing enterocolitis, infantile colic, Helicobacter pylori infection, and irritable bowel syndrome, but further studies are needed to identify which strain(s) is/are the most effective. Data on safety, particularly long-term safety, are limited. The risk of side effects is greater in people who have severe underlying health conditions. Conclusions: The evidence on bacterial and yeast probiotics has considerably expanded during recent years. Accumulated data allow one to make informed decisions about the effectiveness of probiotics and about how to reduce the use of those without proven efficacy.

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“…The unnecessary use of antibiotics promotes global health problems, such as dysbiosis and bacterial resistance to antibiotics . Ingestion of antibiotic produces gut dysbiosis (namely alteration of the function and diversity of bacteria in the intestinal microbiome) in patients . In addition, dysbiosis of the gut microbiome can be induced by other factors, such as type of diet and lifestyle habits .…”

mentioning

confidence: 99%

“…Antibiotic‐associated diarrhoea (AAD) may occur during antibiotic treatment but also after the antibiotic has been discontinued . A high risk for the development of AAD is caused by the administration of aminopenicillins with/without clavulanic acid, cephalosporins, clindamycin, fluoroquinolones or antibiotics with activity against anaerobes . Consequently, AAD is a visible sign of dysbiosis associated with antibiotics when other causes of diarrhoea are absent.…”

mentioning

confidence: 99%

Should dental care professionals prescribe probiotics for their patients under antibiotic administration?

Aragón-Martínez

Martinez-Morales

Bologna‐Molina³

et al. 2019

International Dental Journal

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Systematic review with meta-analysis:Lactobacillus rhamnosusGG in the prevention of antibiotic-associated diarrhoea in children and adults

Abstract: SUMMARY BackgroundThe effects of probiotics are strain specific. The clinical effects of each strain need to be evaluated separately.

Cited by 166 publications

References 27 publications

In silico Prediction, in vitro Antibacterial Spectrum, and Physicochemical Properties of a Putative Bacteriocin Produced by Lactobacillus rhamnosus Strain L156.4

In silico Prediction, in vitro Antibacterial Spectrum, and Physicochemical Properties of a Putative Bacteriocin Produced by Lactobacillus rhamnosus Strain L156.4

Probiotic Bacterial and Fungal Strains: Claims with Evidence

Should dental care professionals prescribe probiotics for their patients under antibiotic administration?

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