Systematic review on the proteomic profile of <i>Mycobacterium tuberculosis</i> exposed to drugs

Campanerut‐Sá, Paula Aline Zanetti; Ghiraldi-Lopes, Luciana Dias; Meneguello, Jean Eduardo; Teixeira, Jorge Juarez Vieira; Scodro, Regiane Bertin de Lima; Siqueira, Vera Lúcia Dias; Svidzinski, Terezinha Inez Estivalet; Pavan, Fernando Rogério; Cardoso, Rosilene Fressatti

doi:10.1002/prca.201600077

Cited by 2 publications

(1 citation statement)

References 27 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(37) Protein profiles provide a map of the biochemical status of stressed cells and organisms closely related to overall phenotypic responses. (42,43) In this work, a comparative proteomics analysis was employed to dissect the mechanisms of toxicity of CdS QDs in S. cerevisiae. We have investigated the most significant responses of this yeast to sublethal concentrations of CdS QDs at two time points, 9 and 24 h, by using 2D-gel electrophoresis followed by MALDI TOF MS-MS as well as the quantitative iTRAQ proteomics approach followed by LC-MS.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis to identify markers of exposure to cadmium sulphide quantum dots (CdS QDs)

Gallo

Srivast

Bulone

et al. 2020

Preprint

View full text Add to dashboard Cite

Background The increasing use of cadmium sulphide (CdS) quantum dot (QD)-enabled products is expected to be accompanied by their release into the environment. In this study, the whole organism Saccharomyces cerevisiae was used as a model eukaryote to study protein modulations employing 2D- gel electrophoresis and gel-free iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) proteomics following cell exposure to CdS QDs for 9 and 24 h. From both biotechnological and ecotoxicological perspectives, the use of S. cerevisiae as a model organism sheds light on the impact nanomaterials have on the biochemical responses of the exposed organism. Results Key proteins involved in essential biological pathways were downregulated, in particular after 24 h exposure. These include the major proteins of the glycolytic pathway, the components of mitochondrial respiratory chain complexes III, IV and V involved in the oxidative phosphorylation chain, the ATP-dependent molecular chaperone Hsc82 as well as other proteins responsible for protein folding and ubiquitination in the endoplasmic reticulum. Some of the proteins whose expression was altered have previously been described as strongly-adsorbed by CdS QD nanomaterial surfaces as hard corona, and involved in the cytotoxicity of this class of engineered nanomaterials. These data may be extrapolated to broader contexts and a wider range of organisms by allowing the identification of robust biomarkers of exposure to CdS QDs. Conclusions The work shows the power of the model organisms S. cerevisiae in biotechnology to ensure high levels of health and environmental safety. In fact, the double proteomic approach allowed to identify early markers of exposure to CdS QDs among all the proteins reprogrammed by the treatment.

show abstract

Section: Introductionmentioning

confidence: 99%

Proteomic analysis to identify markers of exposure to cadmium sulphide quantum dots (CdS QDs)

Gallo

Srivast

Bulone

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

Mycobacterium tuberculosis H37Rv expresses differential proteome during intracellular survival within alveolar epithelial cells compared with macrophages

Agarwal

Ghosh

Sharma

et al. 2018

Pathogens and Disease

View full text Add to dashboard Cite

Mycobacterium tuberculosis survives within the infected host cells by escaping phagolysosomal fusion and adaptation to hosts' intracellular milieu. Recently, the role of alveolar epithelial cells (AECs) in the survival of intracellular M. tuberculosis has gained importance. It has been reported that M. tuberculosis causes cytotoxicity in AECs, a phenotype attributed to M. tuberculosis virulence that could be due to the differential gene/protein expression of bacilli in these cells. Thus, the present study focused on comparative proteomic analysis of intracellular mycobacteria within macrophages and epithelial cells. Intracellular mycobacteria from infected alveolar epithelial (A549) and macrophage (THP-1) cell lines were harvested, and two-dimensional polyacrylamide gel electrophoresis of mycobacterial proteins was performed. The protein spots of interest were picked and MALDI-TOF/MS was performed to identify proteins. Differential expression of 11 mycobacterial proteins was found in infected AECs, out of which six proteins were confirmed by MS/MS analysis. The identified proteins mainly belonged to functional categories of virulence, detoxification and adaptation, and are known to take part in metabolism and respiration, maintenance of virulence and survival during stress. These proteins were predicted to contain peptide motifs possessing cell-penetration ability. Expression of such proteins by intracellular mycobacteria could be essential for survival and persistence in AECs in its virulent state, thus contributing to disease pathogenesis.

show abstract

Systematic review on the proteomic profile of Mycobacterium tuberculosis exposed to drugs

Cited by 2 publications

References 27 publications

Proteomic analysis to identify markers of exposure to cadmium sulphide quantum dots (CdS QDs)

Proteomic analysis to identify markers of exposure to cadmium sulphide quantum dots (CdS QDs)

Mycobacterium tuberculosis H37Rv expresses differential proteome during intracellular survival within alveolar epithelial cells compared with macrophages

Contact Info

Product

Resources

About