Systematic review and meta‐analysis: non‐invasive detection of non‐alcoholic fatty liver disease related fibrosis in the obese

Ooi, Geraldine; Mgaieth, S.; Eslick, Guy D.; Burton, Paul R.; Kemp, William; Roberts, Stuart K.; Brown, Wendy A.

doi:10.1111/obr.12628

Cited by 24 publications

(16 citation statements)

References 57 publications

(109 reference statements)

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“…Prevalence of NAFLD increases dramatically with age, although this disease appears in different age groups (Zhou et al., 2019). There are strong evidences suggesting steatohepatitis and fibrosis are associated with aging, which results in a higher mortality in elderly individuals with NAFLD (Argo et al., 2009; Ooi et al., 2018). Researchers have identified several mechanisms underlying the age promotion the morbidity of NAFLD.…”

Section: Discussionmentioning

confidence: 99%

Nicotinamide riboside exerts protective effect against aging-induced NAFLD-like hepatic dysfunction in mice

Han

Bao

Lou

et al. 2019

PeerJ

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Background & Aims Aging is one of the risk factors of non-alcoholic fatty liver disease (NAFLD). Yet, the mechanism underlying the aging-associated NAFLD-like syndrome is not fully understood. Nicotinamide adenine dinucleotide (NAD), a ubiquitous coenzyme, has protective effects against aging. Here, we investigated the actions of NAD precursors nicotinamide riboside (NR) on the development of aging-induced NAFLD. Methods NR supplemented food (2.5 g/kg food) was applied to aged mice for three months while normal chow to the other groups. Body weight, food intake, liver weight and fat pat mass were measured. The serum concentrations of lipid content, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and NAD were determined by biochemical assays. Pathological assessment and immunohistochemistry analysis of hepatic tissues were used to evaluate the effect of NR on NAFLD development and inflammatory infiltration. Results NR repletion significantly reduced fat pat mass in aged mice, while not altered the body weight, food intake, and liver weight. NR repletion significantly rescued the NAD reduction in aged mice. The total cholesterol and triglyceride levels could be lowered by NR repletion in aged mice. The AST level was also significantly reduced by NR repletion in aged group, while the ALT level lowered but without significance. Notably, moderate NAFLD phenotypes, including steatosis and hepatic fibrosis could be markedly corrected by NR repletion. In addition, Kupffer cells accumulated and inflammatory infiltration could also be remarkably reversed by NR repletion in aged mice. Conclusion Aging was associated with NAFLD-like phenotypes in mice, which could be reversed by oral NR repletion. Therefore, oral NR uptake might be a promising strategy to halt the progression of NAFLD.

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Section: Discussionmentioning

confidence: 99%

Nicotinamide riboside exerts protective effect against aging-induced NAFLD-like hepatic dysfunction in mice

Han

Bao

Lou

et al. 2019

PeerJ

View full text Add to dashboard Cite

show abstract

“…Prevalence of NAFLD increases dramatically with age, although this disease appears in different age groups (Zhou and Li, 2019). There are strong evidences suggesting steatohepatitis and fibrosis are associated with aging, which results in a higher mortality in elderly individuals with NAFLD (Argo and Northup, 2009;Ooi and Mgaieth, 2018). Researchers have identified several mechanisms underlying the age promotion the morbidity of NAFLD.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #2 of "Nicotinamide riboside exerts protective effect against aging-induced NAFLD-like hepatic dysfunction in mice (v0.1)"

2019

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Background & Aims. Aging is one of the risk factors of non-alcoholic fatty liver disease (NAFLD). Yet, the mechanism underlying the aging-associated NAFLD-like syndrome is not fully understood. Nicotinamide adenine dinucleotide (NAD), a ubiquitous coenzyme, has protective effects against aging. Here, we investigated the actions of NAD precursors nicotinamide riboside (NR) on the development of aging-induced NAFLD. Methods. NR supplemented food (2.5g/kg food) was applied to aged mice for three months while normal chow to the other groups. Body weight, food intake, liver weight and fat pat mass were measured. The serum concentrations of lipid content, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and NAD were determined by biochemical assays. Pathological assessment and immunohistochemistry analysis of hepatic tissues were used to evaluate the effect of NR on NAFLD development and inflammatory infiltration. Results. NR repletion significantly reduced fat pat mass in aged mice, while not altered the body weight, food intake, and liver weight. NR repletion significantly rescued the NAD reduction in aged mice. The total cholesterol and triglyceride levels could be lowered by NR repletion in aged mice. The AST level was also significantly reduced by NR repletion in aged group, while the ALT level lowered but without significance. Notably, moderate NAFLD phenotypes, including steatosis and hepatic fibrosis could be markedly corrected by NR repletion. In addition, Kupffer cells accumulated and inflammatory infiltration could also be remarkably reversed by NR repletion in aged mice. Conclusion. Aging was associated with NAFLD-like phenotypes in mice, which could be reversed by oral NR repletion. Therefore, oral NR uptake might be a promising strategy to halt the progression of NAFLD.

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“…Obesity is a significant and rapidly increasing global health issue (Wewege et al, ), affecting approximately 650 million adults worldwide (approximately 13% of the population) (World Health Organization, ). It is associated with several comorbidities, such as type 2 diabetes mellitus (American Diabetes, ), cardiovascular diseases (Parto and Lavie, ), cancer (Berger, ), dyslipidemia (Silva Figueiredo et al, ), nonalcoholic fatty liver disease (Ooi et al, ), osteoarthritis (Vina and Kwoh, ), and is characterized by increased adiposity (Galic et al, ). The increase of adipose mass is determined by the balance between lipogenesis and lipolysis (Coelho et al, ).…”

Section: Introductionmentioning

confidence: 99%

Identification of Suitable Reference Genes for Quantitative Gene Expression Analysis in Innervated and Denervated Adipose Tissue from Cafeteria Diet‐Fed Rats

Marques-Oliveira

Silva

Valadares

et al. 2019

Lipids

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Our previous studies show that cafeteria diet increases body adiposity, plasma insulin levels, and sympathetic activity to brown adipose tissue (BAT) and white adipose tissue (WAT) of Wistar rats, leading to rapid and progressive changes in the metabolic profile. The identification of suitable reference genes that are not affected by the experimental conditions is a critical step in accurate normalization of the reverse transcription quantitative real‐time PCR (qRT‐PCR), a commonly used assay to elucidate changes in the gene expression profile. In the present study, the effects of the cafeteria diet and sympathetic innervation on the gene expression of adrenoceptor beta 3 (Adrb3) from BAT and WAT were assessed using one of the most stable and one of the least stable genes as normalizers. Rats were fed the cafeteria diet and on the 17th day, interscapular BAT or retroperitoneal WAT was denervated and, 7 days after surgery, the contralateral innervated tissue was used as control. Ten reference genes were evaluated (18S, B2m, Actb, CypA, Gapdh, Hprt1, Rpl32, Tbp, Ubc, and Ywhaz) and ranked according to their stability using the following algorithms: geNorm, NormFinder, BestKeeper, and comparative delta threshold cycle (ΔC t) method. According to the algorithms employed, the normalization of Adrb3 expression by the least stable genes produced opposite results compared with the most stable genes and literature data. In cafeteria and control diet‐fed rats, the three most stable genes were Hprt1, Tbp, and Rpl32 for interscapular BAT and Tbp, B2m, and Hprt1 for retroperitoneal WAT, while the least stable genes were 18S, Actb, and Gapdh for both tissues.

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Systematic review and meta‐analysis: non‐invasive detection of non‐alcoholic fatty liver disease related fibrosis in the obese

Abstract: In obese subjects, complex biomarker panels and elastography have been reasonable to good accuracy for NAFLD-related fibrosis; however, these methods have not been well validated. Further study in this high-risk population is needed.

Cited by 24 publications

References 57 publications

Nicotinamide riboside exerts protective effect against aging-induced NAFLD-like hepatic dysfunction in mice

Nicotinamide riboside exerts protective effect against aging-induced NAFLD-like hepatic dysfunction in mice

Peer Review #2 of "Nicotinamide riboside exerts protective effect against aging-induced NAFLD-like hepatic dysfunction in mice (v0.1)"

Identification of Suitable Reference Genes for Quantitative Gene Expression Analysis in Innervated and Denervated Adipose Tissue from Cafeteria Diet‐Fed Rats

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