Systematic overestimation of human serum albumin by capillary zone electrophoresis method due to monoclonal immunoglobulin interferences

Padelli, Maël; Labouret, Théo; Labarre, Marion; Reun, Eymeric Le; Rouillé, Amélie; Kerspern, Hélène; Capaldo, Clément; Chauvet, Juliette; Plée-Gautier, Emmanuelle; Carré, Jean‐Luc; Leven, Cyril

doi:10.1016/j.cca.2019.01.010

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…42 When compared with other methods, including nephelometry and bromocresol purple, CZE was found to overestimate human albumin slightly when the albumin was less than 3 g/dL and also when monoclonal immunoglobulins were present. 43,44 The use of CZE in veterinary medicine needs to be more fully evaluated, given the low levels of albumin and higher levels of globulins found in many avian and reptile species. Higher albumin levels are also observed by CZE in dogs, cats, and some bird species.…”

Section: Valid Quantitation Of Albuminmentioning

confidence: 99%

Protein electrophoresis of non‐traditional species: A review

Cray

2021

Veterinary Clinical Pathol

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EPH can provide information regarding serum or plasma proteins, including albumin and globulins, that can be broadly classified based on their migration. The globulin fractions can reveal inflammation as well as polyclonal and monoclonal gammopathies. Detection of the latter, also named paraproteins, is one of the most common applications of EPH in human medicine as clinicians have a large toolbox of specific assays for various acute-phase proteins (APPs) that allow them to more specifically gauge inflammation. 1,2 In veterinary medicine, given the advances in species-specific and cross-reactive reagents for major APP in traditional species, including dogs, cats, and horses, these tools can complement the use of EPH in its valid quantitation of albumin and detection of paraproteinemia. [3][4][5][6][7][8] In non-traditional species, including birds, reptiles, fish, amphibians, invertebrates, small exotic mammals, and non-domesticated mammals, EPH can also be used as an aid to the diagnosis of lymphoproliferative disease, but its primary use is in the detection of inflammation and polyclonal gammopathies. For many of these species, reagents are not yet available or widely validated to quantitate APPs. The valid quantitation of albumin, a key negative APP, by biochemistry analyzer methods, remains a major issue. Moreover, in non-mammalian species, as innate immunity is perhaps the essential, diverse, and robust immune response, EPH can provide even more value. 9,10 Challenges in the applications of EPH in these special species include a lack of standardization of fraction delimiter placements, RIs, and information regarding fraction composition. This paper reviews the applications of EPH in non-traditional species as well as the currently available methodology. Additional reviews on this subject with a varied focus are also available. [11][12][13][14][15] | APPLI C ATI ON S OF EPH IN NON -TR ADITIONAL S PECIE S | Detection of monoclonal gammopathyAs described in mammals, EPH is a key tool in the detection of paraproteins. 2,3 This application has also been demonstrated in nontraditional species. 16,17 In electrophoretograms of avian plasma,

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Section: Valid Quantitation Of Albuminmentioning

confidence: 99%

Protein electrophoresis of non‐traditional species: A review

Cray

2021

Veterinary Clinical Pathol

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“…A positive bias has been reported in the measurement of human serum albumin concentration with CZE compared to bromocresol purple, turbidimetry, and nephelometry methods. 32,33 In the control group of our study (group B), the comparison of the 2 methods revealed a significant difference between CZE and BCG albumin concentrations, with higher CZE albumin concentrations. In dogs, it was suggested that the serum albumin concentration measured with the BCG method tended to be higher compared to SPE.…”

Section: Discussionmentioning

confidence: 54%

Spurious capillary zone electrophoresis pattern in hypercholesterolemic dogs

Mangiagalli¹,

Meazzi

Giordano

et al. 2022

J VET Diagn Invest

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Capillary zone electrophoresis (CZE) is a relatively new serum protein electrophoresis method with higher resolution than other electrophoretic techniques. Hypercholesterolemic dogs exhibit a peculiar CZE pattern. Specifically, they have a shoulder or peak immediately next to the albumin peak. We investigated the prevalence of this spurious peak in hypercholesterolemic dogs and its correlation with the serum cholesterol concentration. Moreover, possible discrepancies between the CZE and spectrophotometric (bromocresol green [BCG] method) albumin concentrations in those animals were evaluated, as well as the accuracy in measuring albumin by a different CZE fractionation system. We retrospectively enrolled 500 hypercholesterolemic and normotriglyceridemic dogs. Each electrophoretic curve was inspected visually to identify a spurious peak (prevalence of 68.8%). We chose 120 dogs to further investigate the albumin concentration; CZE albumin was significantly higher than measured using the BCG method. A weak but significant correlation ( r = 0.412; p < 0.0001) was observed between the magnitude of the spurious peak and the serum cholesterol concentration. Finally, the significant difference between CZE and BCG albumin measurement disappeared ( p = 0.92) when the spurious peak was considered as α1-globulins instead of albumin.

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“…Generally, alpha-1 antichymotrypsin, alpha-1 antitrypsin, and alpha-1 acid glycoprotein (orosomucoid) migrate in the alpha-1 globulin fraction. Ceruloplasmin and haptoglobin migrate to the alpha-2 globulin fraction, and C-reactive protein (CRP) migrates to the gamma globulin fraction [15]. These fractions increase proportionally during the inflammatory syndrome [16].…”

Section: Resultsmentioning

confidence: 99%

Serum Protein Electrophoretic in Children

Hadrach

Benazzouz

2023

International Journal of Pediatrics

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Serum protein electrophoresis is a simple, reliable, and specific method used for separation of serum proteins. This study consisted to detect, at pediatric cases, pathological profiles of serum proteins by capillary electrophoresis and interpret any anomalies. The study was performed on 81 sera collected from pediatric subjects admitted at the Abderrahim Harouchi Children’s Hospital in Casablanca. Study results revealed 72 specific pathological electrophoretic patterns for acute and chronic inflammatory response (35 children), hypogammaglobulinemia (3), polyclonal hypergammaglobulinemia (23), hypoalbuminemia (5), agammaglobulinemia (1), and other medical conditions (2). No cases of alpha-1-antitrypsin deficiency and nephrotic syndrome by electrophoresis were highlighted. Serum protein electrophoresis in children is recommended as a diagnostic technique for increasing the accuracy of the diagnosis in acute, subacute, and chronic inflammatory diseases, liver disease, and cases of immunodeficiency.

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Systematic overestimation of human serum albumin by capillary zone electrophoresis method due to monoclonal immunoglobulin interferences

Cited by 7 publications

References 34 publications

Protein electrophoresis of non‐traditional species: A review

Protein electrophoresis of non‐traditional species: A review

Spurious capillary zone electrophoresis pattern in hypercholesterolemic dogs

Serum Protein Electrophoretic in Children

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