Systematic mining of salt-tolerant genes in halophyte-Zoysia matrella through cDNA expression library screening

Chen, Yu; Zong, Jingfeng; Tan, Zhiqun; Li, Lanlan; Hu, Baoyun; Chen, Chuanming; Chen, Jingbo; Liu, Jianxiu

doi:10.1016/j.plaphy.2015.02.007

Cited by 31 publications

(25 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The overexpression of the genes HcVP1 and HcVHA-B in Arabidopsis improved salt tolerance through the increased accumulation of Na + in vacuoles (Hu et al, 2012). Recently Chen et al (2015b) reported that the improved salttolerant ability in salt-sensitive yeast strain and also in Arabidopsis by inserting the Zoysia matrella vacuolar pyrophosphatase (ZmVP1). Authors also noticed that the higer levels of expression of different stress related genes such as AtNHX1, AtLEA, ATP5CS, AtMn-SOD and AtAPX1 which is an indication of higher levels of activities such as sodium sequesteration into vacuole, assimilation of potassium, osmotic regulation and antioxidant activities of transgenic plants.…”

Section: Sodium Tolerance/regulation For Salt Tolerancementioning

confidence: 99%

Salt-tolerant genes from halophytes are potential key players of salt tolerance in glycophytes

Himabindu

Chakradhar

Reddy

et al. 2016

Environmental and Experimental Botany

165

109

View full text Add to dashboard Cite

Crop productivity strongly depends on several biotic and abiotic factors. Salinity is one of the most important abiotic factors, besides drought, extreme temperatures, light and metal stress. The enhanced burden of secondary salinization induced through anthropogenic activities increases pressure on glycophytic crop plants. The recent isolation and characterization of salt tolerance genes encoding signaling components from halophytes, which naturally grow in high salinity, has provided tools for the development of transgenic crop plants with improved salt tolerance and economically beneficial traits. In addition understanding of the differences between glycophytes and halophytes with respect to levels of salinity tolerance is also one of the prerequisite to achieve this goal. Based on the recent developments in mechanisms of salt tolerance in halophytes, we will explore the potential of introducing salt tolerance by choosing the available genes from both dicotyledonous and monocotyledonous halophytes, including the salt overly sensitive system (SOS)-related cation/proton antiporters of plasma (NHX/SOS1) and vacuolar membranes (NHX), energy-related pumps, such as plasma membrane and vacuolar H + adenosine triphosphatase (PM & V-H + ATPase), vacuolar H + pyrophosphatases (V-H + PPase) and potassium transporter genes. Various halophyte genes responsible for other processes, such as crosstalk signaling, osmotic solutes production and reactive oxygen species (ROS) suppression, which also enhance salt tolerance will be described. In addition, the transgenic overexpression of halophytic genes in crops (rice, peanut, finger millet, soybean, tomato, alfalfa, jatropha, etc.) will be discussed as a successful mechanism for the induction of salt tolerance. Moreover, the advances in genetic engineering technology for the production of genetically modified crops to achieve the improved salinity tolerance under field conditions will also be discussed. 2015 Elsevier B.V. All rights reserved.

show abstract

Section: Sodium Tolerance/regulation For Salt Tolerancementioning

confidence: 99%

Salt-tolerant genes from halophytes are potential key players of salt tolerance in glycophytes

Himabindu

Chakradhar

Reddy

et al. 2016

Environmental and Experimental Botany

165

109

View full text Add to dashboard Cite

show abstract

“…However, due to the lack of genomic information for non-model plant species, the changes in transcriptomic, proteomic, or metabolomic processes caused by stress do not provide sufficient information for the cloning and isolation of stress-related genes in non-model plant species. The F ull-length cDNA Over-eXpressor (FOX) gene hunting system is a functional screening method using the overexpression of full-length cDNA libraries in model plants ( Arabidopsis or rice) or microorganisms (mainly yeast) [ 13 , 14 , 15 , 16 , 17 ], by which some unknown stress-related genes have been cloned and preliminarily identified. Actually, in much earlier studies, the functional screening work with yeast mutants was widely used to separate salt tolerance genes from plant cDNA libraries due to the lack of any genomic sequence information, even in model plants such as Arabidopsis [ 18 , 19 ], tobacco [ 20 ], or rice [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…Since yeast ( Saccharomyces cerevisiae ) is a unicellular organism with the best research background, the functional screening of stress-tolerance-related genes in yeast with the FOX gene hunting system seems more simple and easier to operate than in model plants (such as Arabidopsis and rice). In recent years, this gene hunting system has also been widely applied to isolate salt tolerance genes in some extreme halophyte species without any genomic sequence information [ 15 , 16 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Identification of Salt-Stress-Related Genes Using the FOX Hunting System from Ipomoea pes-caprae

Zhang

Zheng

et al. 2018

IJMS

View full text Add to dashboard Cite

Ipomoea pes-caprae is a seashore halophytic plant and is therefore a good model for studying the molecular mechanisms underlying salt and stress tolerance in plant research. Here, we performed Full-length cDNA Over-eXpressor (FOX) gene hunting with a functional screening of a cDNA library using a salt-sensitive yeast mutant strain to isolate the salt-stress-related genes of I. pes-caprae (IpSR genes). The library was screened for genes that complemented the salt defect of yeast mutant AXT3 and could grow in the presence of 75 mM NaCl. We obtained 38 candidate salt-stress-related full-length cDNA clones from the I. pes-caprae cDNA library. The genes are predicted to encode proteins involved in water deficit, reactive oxygen species (ROS) scavenging, cellular vesicle trafficking, metabolic enzymes, and signal transduction factors. When combined with the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses, several potential functional salt-tolerance-related genes were emphasized. This approach provides a rapid assay system for the large-scale screening of I. pes-caprae genes involved in the salt stress response and supports the identification of genes responsible for the molecular mechanisms of salt tolerance.

show abstract

“…However, the genes underlying Cd tolerance are largely unknown. Thirty-two full length salinity-tolerance related genes from physic nut ( Jatropha curcas ) and sixteen salinity-tolerance related genes from zoysiagrass ( Zoysia matrella ) were isolated with the yeast FOX-gene hunting system (Eswaran et al, 2010 ; Chen et al, 2015b ). OsMPG1 and OsKAT1 were also identified in rice and confirmed for salinity tolerance (Obata et al, 2007 ; Kumar et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Functional Identification and Characterization of Genes Cloned from Halophyte Seashore Paspalum Conferring Salinity and Cadmium Tolerance

Chen

Tan

et al. 2016

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Salinity-affected and heavy metal-contaminated soils limit the growth of glycophytic plants. Identifying genes responsible for superior tolerance to salinity and heavy metals in halophytes has great potential for use in developing salinity- and Cd-tolerant glycophytes. The objective of this study was to identify salinity- and Cd-tolerance related genes in seashore paspalum (Paspalum vaginatum), a halophytic perennial grass species, using yeast cDNA expression library screening method. Based on the Gateway-compatible vector system, a high-quality entry library was constructed, which contained 9.9 × 106 clones with an average inserted fragment length of 1.48 kb representing a 100% full-length rate. The yeast expression libraries were screened in a salinity-sensitive and a Cd-sensitive yeast mutant. The screening yielded 32 salinity-tolerant clones harboring 18 salinity-tolerance genes and 20 Cd-tolerant clones, including five Cd-tolerance genes. qPCR analysis confirmed that most of the 18 salinity-tolerance and five Cd-tolerance genes were up-regulated at the transcript level in response to salinity or Cd stress in seashore paspalum. Functional analysis indicated that salinity-tolerance genes from seashore paspalum could be involved mainly in photosynthetic metabolism, antioxidant systems, protein modification, iron transport, vesicle traffic, and phospholipid biosynthesis. Cd-tolerance genes could be associated with regulating pathways that are involved in phytochelatin synthesis, HSFA4-related stress protection, CYP450 complex, and sugar metabolism. The 18 salinity-tolerance genes and five Cd-tolerance genes could be potentially used as candidate genes for genetic modification of glycophytic grass species to improve salinity and Cd tolerance and for further analysis of molecular mechanisms regulating salinity and Cd tolerance.

show abstract

Systematic mining of salt-tolerant genes in halophyte-Zoysia matrella through cDNA expression library screening

Cited by 31 publications

References 43 publications

Salt-tolerant genes from halophytes are potential key players of salt tolerance in glycophytes

Salt-tolerant genes from halophytes are potential key players of salt tolerance in glycophytes

Functional Identification of Salt-Stress-Related Genes Using the FOX Hunting System from Ipomoea pes-caprae

Functional Identification and Characterization of Genes Cloned from Halophyte Seashore Paspalum Conferring Salinity and Cadmium Tolerance

Contact Info

Product

Resources

About