Systematic investigation for the mechanisms and the substance basis of Yang–Xin–Ding–Ji capsule based on the metabolite profile and network pharmacology

Abstract

Because traditional Chinese medicine (TCM) is a complex mixture of multiple components, the application of methodologies for evaluating single-component Western medicine in TCM studies may have certain limitations. Appropriate strategies that recognize the integrality of TCM and connect to TCM theories remain to be developed. Yang-Xin-Ding-Ji (YXDJ) capsule is originally from a classical TCM formula used for the treatment of arrhythmia. In this study, we used UPLC-Q-TOF-MS detection method, coupled with the metabolic research and network pharmacology analysis, to study the scientific connotation of the YXDJ capsule. A total of 33 absorbed constituents and 23 metabolites were identified or tentatively characterized in dosed plasma and urine, and the possible metabolic pathways were mainly methylation, oxidation, sulfation, glucuronidation, and deglucosylation. We optimized the conventional process ways of network pharmacology by collecting targets based on absorbed constituents into the blood. The constituents-target disease and Kyoto Encyclopedia of Genes pathway analysis revealed that 24 absorbed constituents, 32 target genes, and 10 key pathways were probably related to the efficacy of the YXDJ capsule against arrhythmia. The results provided a scientific basis for understanding the bioactive compounds and the pharmacological mechanism of the YXDJ capsule.

Curative Effect of Yangxin Dingji Capsule Combined With Mexiletine Hydrochloride on Postoperative Arrhythmia and Its Influences on the Vascular Endothelial Function in Coronary Bifurcation Lesions

“…(1) After 4 weeks of treatment, the clinical efficacy evaluation was carried out with reference to the relevant basis in the Guidelines for Clinical Research of New Chinese Medicines [ 5 ]. The main clinical symptoms of the evaluation were chest tightness and fatigue, palpitations, shortness of breath, dizziness, and insomnia, and the number of arrhythmias was recorded by an electrocardiogram.…”

“…A total of 110 CBL patients admitted to our hospital from January 2021 to December 2021 were selected as the research subjects, and they were randomly divided into the combined group and the control group, with 55 cases in each group. Inclusion criteria are as follows: (1) all patients met the CBL-related diagnostic criteria in "Interventional Treatment of Coronary Artery Bifurcation Lesions" [5]; (2) there were no detours or severe calcification in branch or main blood vessels; (3) all patients were included in the study, in line with PCI indications; (4) all patients had arrhythmias after ECG detection; (5) this study was approved by the patients and their families, and was approved by the Medical Ethics Committee of our hospital. Exclusion criteria are as follows: (1) combined with other malignant tumors; (2) suffering from autoimmune diseases; (3) allergic to mexiletine hydrochloride or Yangxin Dingji capsule; and (4) patients with serious abnormal mental and cognitive functions.…”

Herbal Granules of Heat-Clearing and Detoxifying for Children with Mild Hand, Foot, and Mouth Disease: A Bayesian Network Meta-Analysis

“…Limited by the included trial number of each HGs-HD, other methods to further decrease heterogeneity could not be conducted in our study. Thirdly, since traditional Chinese medicine (TCM) is a complex mixture of multiple components [ 44 ], the effects of HGs-HD were not fully enough to attribute to a single Chinese herb or ingredient, and the mechanism and material basis were needed to be further explored [ 40 ]. Fourthly, given the nature of the indirect comparisons of NMA, it remains a surrogate for head-to-head RCTs.…”