Systematic Identification and Analysis of NAC Gene Family in Moso Bamboo (Phyllostachys edulis)

Abstract

Background: NAC (NAM/ATAF1/2/CUC2) gene family is a large plant-specific transcription factor family, which is implicated in many functions, such as morphogenesis, the thickness formation of secondary cell walls as well as biotic and abiotic stress and more. In moso bamboo ( Phyllostachys edulis ), 94 PeNACs have been identified and three members are predicted to relate to the secondary cell wall. However, there were few studies on moso bamboo NAC genes under stress.Results: In this study, we re-identified 165 PheNACs with the latest moso bamboo genome data and divided them into 12 subfamilies using NAM domains. Gene structure and motif distribution manifested the NAC gene family was fairly conserved. Evolutionary analysis showed that the segmental duplication played a significant role in the expansion of NAC genes and the relationship between moso bamboo and Brachypodium distachyon was closest than beween moso bamboo and other four species ( Arabidopsis thaliana, Oryza sativa , Sorghum bicolor and Zea mays ). Based on the promoter analysis of the 27 NAC members in A subfamily, quantitative real-time PCR exhibited these genes reacted differently under drought, high salt, abscisic acid and methyl jasmonate treatments. Finally, we selected out four potential stress-associated genes (PheNAC001, -056, -080 and -100) and found they all localized in the tobacco nucleus and had transcriptional activity in yeast.Conclusions: These preliminary results provide valuable information for mining potential resistance NAC genes and lay theoretical basis for breeding new stress-resistant varieties in moso bamboo.

Entailing the Next-Generation Sequencing and Metabolome for Sustainable Agriculture by Improving Plant Tolerance

“…Furthermore, abiotic stresses create a serious impact on bamboo growth and development [213]. Access to the moso bamboo genome [210,211] provides a chance to many researchers for genome-wide classifications of TFs such as aquaporin, AAAP, UBP, IQD, HD-Zip, Hexokinase, Aux/IAA and ARF, NAC, PeUGE, HSF, and CONSTANS-like in moso bamboo [188,[214][215][216][217][218][219][220][221][222][223]. Genome-wide classifications of TFs' families in moso bamboo have been carried out and have demonstrated a limited molecular characterization in model plants by exogenous gene transfer in rice and Arabidopsis and the expression profile against stresses [224][225][226].…”

“…Furthermore, abiotic stresses create a serious impact on bamboo growth and development [ 213 ]. Access to the moso bamboo genome [ 210 , 211 ] provides a chance to many researchers for genome-wide classifications of TFs such as aquaporin, AAAP, UBP, IQD, HD-Zip, Hexokinase, Aux/IAA and ARF, NAC, PeUGE, HSF, and CONSTANS-like in moso bamboo [ 188 , 214 , 215 , 216 , 217 , 218 , 219 , 220 , 221 , 222 , 223 ].…”