Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata

Abstract

The various post-translational modifications of plant proteins have important regulatory roles in development. We therefore examined various modified proteins from strawberry stigmata and found that succinylation of lysine residues was the most abundant type of modification. We then subjected proteins from strawberry stigmata to an efficient enrichment method for succinylated peptides and identified 200 uniquely succinylated lysines in 116 proteins. A bioinformatics analysis revealed that these proteins are involved in important biological processes, including stress responses, vesicular transport, and energy metabolism. Proteomics, combined with immunoprecipitation and immunoblotting, revealed an obvious increase in succinylation of the assembly polypeptide 2 (AP2) and clathrin from 0.5 to 2 h after pollination, suggesting that succinylation is involved in the recognition of pollen-stigma signaling substances and vesicular transport. These results suggest that AP2/clathrin-mediated vesicular transport processes are regulated by lysine succinylation during pollen recognition.

Systematic analysis of the lysine succinylome in the model medicinal mushroom Ganoderma lucidum

“…So far, large amounts of lysine succinylated proteins have been found in both prokaryotes and eukaryotes [7, 11–16]. However, the conservation of lysine succinylome in these species is still unclear.…”

“…Therefore, proteins with K or R in corresponding sites are more easily to be the preferred substrates of lysine succinyltransferase in G. lucidum . It is noteworthy that the conserved motifs form strawberry stigmata [11], common wheat [7], C. albicans [12], and V. parahemolyticus [14] are different, indicating that different species contain unique succinylated proteins with specific functions.…”

“…In the past few years, lysine succinylation has been widely investigated at the level of proteomics thanks to the development of lysine-succinylated peptides immune precipitation technology. Succinylated proteins are found in large quantities in plants [7, 11], yeast [12], fungus [13], and bacteria [14–16]. These proteomic analyses revealed a wide range of roles for succinyl modification in various cellular functions such as catalytic activities, energy generation and photosynthesis in plants.…”

Systematic identification and comparative analysis of lysine succinylation between the green and white parts of chimeric leaves of Ananas comosus var. bracteatus

“…7c). Among these motifs, K su (X4) K and K su (X7) K were previously identified in other plant species [16,18,19,[21][22][23][24][25][26][27][28]32]. Notably, K su (X7) K was also observed in the marine bacterium [33], indicating that some motifs might be conservative between plant and bacteria.…”

“…But the number of succinylated proteins in Ananas comosus var. bracteatus was much higher than that in strawberry stigmata [23], common wheat [24], rice seeds [25], tomato [26], Taxus×media [27], Brachypodium distachyon [28], Dendrobium officinale [29]. In physiological level, different species and tissues may possess differential profile of succinylation.…”