Systematic Comparison of Computational Tools for Sanger Sequencing-Based Genome Editing Analysis
Kanae Aoki,
Mai Yamasaki,
Riku Umezono
et al.
Abstract:Successful genome editing depends on the cleavage efficiency of programmable nucleases (PNs) such as the CRISPR–Cas system. Various methods have been developed to assess the efficiency of PNs, most of which estimate the occurrence of indels caused by PN-induced double-strand breaks. In these methods, PN genomic target sites are amplified through PCR, and the resulting PCR products are subsequently analyzed using Sanger sequencing, high-throughput sequencing, or mismatch detection assays. Among these methods, S… Show more
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