Systematic Characterization of the Group 2 House Dust Mite Allergen in Dermatophagoides microceras

Hu, Rei-Hsing; Wu, Chia-Ta; Wu, Tzu‐Chin; Yu, Feng‐Yih; Ko, Jiunn‐Liang; Ko, Jiunn‐Liang; Liu, Yu-Fan

doi:10.3389/fcimb.2021.793559

Cited by 6 publications

(8 citation statements)

References 51 publications

(69 reference statements)

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“…27 HDMs produce various allergens that trigger an immune response and often possess proteolytic activity. 13 These allergens are associated with bacterial DNA, endotoxin, chitin, and other substances that modulate immune responses. 14 While membrane-bound Toll-like receptors related to host immunity have been extensively studied, there are also other cytosolic receptors known as Nucleotide-binding…”

Section: Discussionmentioning

confidence: 99%

“…Our previous findings indicate an approximately 80% similarity in protein sequences among cross-reactive allergens from three species of Dermatophagoides HDMs. 13,14 When comparing the gene expression profiles of Der m with two other HDM species (Der p and Der f ), Der m showed a tendency to be associated with T1DM signaling pathways (Figure 5). Additionally, significant Der m HDMs allergen presence has been reported in the temperate climate of northern Europe.…”

Section: Discussionmentioning

confidence: 99%

“…12 Conversely, Group 2 allergens have the potential to mimic the Toll-like receptor 4 coreceptor MD-2, exhibiting immunomodulatory effects. 13 In recent years, allergic conditions, particularly those related to asthma, have become increasingly prevalent, partly due to environmental and climatic changes, along with industrialization. These factors influence the proliferation, survival, and allergen production of HDMs, making them significant contributors to allergic diseases.…”

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confidence: 99%

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Multiplexed transcriptional profiling of Dermatophagoides house dust mites allergens in human epithelium cells

Lin,

Cheng,

et al. 2023

Environmental Toxicology

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Allergic asthma, a chronic disease characterized by airway inflammation, poses a significant public health concern. It is well‐established that house dust mites (HDMs) are common inducers of allergic responses in individuals, particularly children. In central Taiwan, our research team observed that over 80% of allergic children exhibited sensitization to various HDMs species. This investigation aims to bridge the gap between these observations and a better understanding of the early fundamental mechanisms for preventing allergic diseases. Specifically, our study delves into the impact of crude extracts of HDMs on human epithelial BEAS‐2B cells. Our findings, based on RNA sequencing (RNA‐seq) analysis, shed light on how three major Dermatophagoides HDMs allergens activate a common Toll‐like receptor signaling pathway in human epithelial cells within a 4‐h treatment. During this process, the nuclear transcription factor NF‐κB translocated into the cell nucleus within 30 min of allergen stimulation, triggering the expression of pro‐inflammatory genes such as IL‐6 and IL‐8 over 4 h. Additionally, when the cells were treated with specific Dermatophagoides microceras (Der m) allergens, it resulted in the upregulation of genes that regulate type 1 diabetes mellitus (T1DM) signaling pathways. This led to the mediation of IL‐12A inflammation. Furthermore, there was an increase in gene sets associated with cilia function and the microtubule cytoskeleton in human epithelial cells after treatment with a combination of Der m allergens and Dexamethasone. Additionally, OMICs analysis was conducted to examine the effects of HDMs allergenic stimulation on human epidermal cells. We aimed to improve our understanding of the molecular mechanisms within cells and identify potential targets and natural products in the treatment of asthma caused by HDMs allergens.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Multiplexed transcriptional profiling of Dermatophagoides house dust mites allergens in human epithelium cells

Lin,

Cheng,

et al. 2023

Environmental Toxicology

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“…Following published protocols, 15,16 we assessed the immunogenicity of recombinant protein rDer p 22 in mice. Six‐ to eight‐week‐old female BALB/c mice (purchased from Shanghai Laboratory Animal Center, SLAC) were maintained in a pathogen‐free facility, and experimental procedures were approved by the Animal Ethic Committee at The Affiliated Wuxi People's Hospital of Nanjing Medical University.…”

Section: Methodsmentioning

confidence: 99%

Dermatophagoides pteronyssinus allergen Der p 22: Cloning, expression, IgE‐binding in asthmatic children, and immunogenicity

Zhou

et al. 2022

Pediatric Allergy Immunology

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Background: Dust mite extract contains multiple components that, while useful in clinical allergy diagnosis and treatment, can cause serious side effects. Defining components of dust mite extract is important their contributions to allergic disease. This study aimed to characterize a novel dust mite allergen, Der p 22. Methods:We amplified the cDNA encoding Der p 22 from total RNA of the mite Dermatophagoides pteronyssinus, and inserted it into an expression construct for transformation to competent cells. Purified recombinant (r) Der p 22 was tested for IgEbinding reactivity in sera obtained from children with allergic asthma by the Affiliated Wuxi Children's Hospital of Nanjing Medical University (Jiangsu, China). rDer p 22 also was used to challenge BALB/c mice to assess effects on T helper cells and cytokine levels and applied to cultured lung epithelial cells to evaluate apoptosis and cytokine secretion.Results: rDer p 22 bound to IgE in 93.75% of sera from pediatric allergic asthma patients. Mice challenged with rDer p 22 had altered Th1/Th2 ratios in spleen and lymph, and lower levels of cytokines IFNγ but higher levels of IL-4 and IL-10 in alveolar lavage fluid compared with controls (p < .05). Cultured lung epithelial cells had greater apoptosis rates and exhibited higher levels of IL-6, IL-8, and GM-CSF when treated with rDer p 22 compared with control treatment (p < .05).Conclusions: Recombinant Der p 22 exhibited high IgE-binding rates in allergic children, indicating the activity of the recombinant protein and suggesting this novel allergen may be appropriate for inclusion in an allergy diagnostic workup. This finding is supported by in vitro and mouse in vivo studies showing rDer p 22 induced strong allergenic reactivity and apoptosis.

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“…Whether the Der m 1 major proteinase-activated immune responses are induced by the generated FCPs was determined. Furthermore, the relationship between proteinase allergen ( Der m 1 ) and another major allergen component ( Der m 2 ) [ 15 ], which is the group 2 allergen of Dermatophagoides and thought to be an MD-2-related lipid-binding protein [ 16 , 17 ], was studied in an animal-based model, in addition to the mechanism of FCPs formed by an allergenic proteinase from Der m 1 in human bronchial epithelium cells.…”

Section: Introductionmentioning

confidence: 99%

Integrated OMICs Approach for the Group 1 Protease Mite-Allergen of House Dust Mite Dermatophagoides microceras

Cheng

et al. 2022

IJMS

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House dust mites (HDMs) are one of the most important allergy-causing agents of asthma. In central Taiwan, the prevalence of sensitization to Dermatophagoides microceras (Der m), a particular mite species of HDMs, is approximately 80% and is related to the IgE crossing reactivity of Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farinae (Der f). Integrated OMICs examination was used to identify and characterize the specific group 1 mite-allergic component (Der m 1). De novo draft genomic assembly and comparative genome analysis predicted that the full-length Der m 1 allergen gene is 321 amino acids in silico. Proteomics verified this result, and its recombinant protein production implicated the cysteine protease and α chain of fibrinogen proteolytic activity. In the sensitized mice, pathophysiological features and increased neutrophils accumulation were evident in the lung tissues and BALF with the combination of Der m 1 and 2 inhalation, respectively. Principal component analysis (PCA) of mice cytokines revealed that the cytokine profiles of the allergen-sensitized mice model with combined Der m 1 and 2 were similar to those with Der m 2 alone but differed from those with Der m 1 alone. Regarding the possible sensitizing roles of Der m 1 in the cells, the fibrinogen cleavage products (FCPs) derived from combined Der m 1 and Der m 2 induced the expression of pro-inflammatory cytokines IL-6 and IL-8 in human bronchial epithelium cells. Der m 1 biologically functions as a cysteine protease and contributes to the α chain of fibrinogen digestion in vitro. The combination of Der m 1 and 2 could induce similar cytokines expression patterns to Der m 2 in mice, and the FCPs derived from Der m 1 has a synergistic effect with Der m 2 to induce the expression of pro-inflammatory cytokines in human bronchial epithelium cells.

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Systematic Characterization of the Group 2 House Dust Mite Allergen in Dermatophagoides microceras

Cited by 6 publications

References 51 publications

Multiplexed transcriptional profiling of Dermatophagoides house dust mites allergens in human epithelium cells

Multiplexed transcriptional profiling of Dermatophagoides house dust mites allergens in human epithelium cells

Dermatophagoides pteronyssinus allergen Der p 22: Cloning, expression, IgE‐binding in asthmatic children, and immunogenicity

Integrated OMICs Approach for the Group 1 Protease Mite-Allergen of House Dust Mite Dermatophagoides microceras

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