Systematic Analysis of MYB Family Genes in Potato and Their Multiple Roles in Development and Stress Responses

Abstract

The MYB proteins represent a large family of transcription factors and play important roles in development, senescence, and stress responses in plants. In the current study, 233 MYB transcription factor-encoding genes were identified and analyzed in the potato genome, including 119 R1-MYB, 112 R2R3-MYB, and two R1R2R3-MYB members. R2R3-MYB is the most abundant MYB subclass and potato R2R3-MYB members together with their Arabidopsis homologs were divided into 35 well-supported subgroups as the result of phylogenetic analyses. Analyses on gene structure and protein motif revealed that members from the same subgroup shared similar exon/intron and motif organization, further supporting the results of phylogenetic analyses. Evolution of the potato MYB family was studied via syntenic analysis. Forty-one pairs of StMYB genes were predicted to have arisen from tandem or segmental duplication events, which played important roles in the expansion of the StMYB family. Expression profiling revealed that the StMYB genes were expressed in various tissues and several StMYB genes were identified to be induced by different stress conditions. Notably, StMYB030 was found to act as the homolog of AtMYB44 and was significantly up-regulated by salt and drought stress treatments. Furthermore, overexpression of StMYB030 in Arabidopsis enhanced salt stress tolerance of transgenic plants. The results from this study provided information for further functional analysis and for crop improvements through genetic manipulation of these StMYB genes.

MYB Superfamily in Brassica napus: Evidence for Hormone-Mediated Expression Profiles, Large Expansion, and Functions in Root Hair Development

“…Four of the newly identified subfamilies (S74, S75, S76, and S77) and S10 were likely to be Brassica -specific because no counterparts were identified in other species ( Figure 2 and Figure S3 ). Similarly, the previously defined A. thaliana -specific subfamily S12 [ 22 , 42 , 43 ], which is specifically involved in the glucosinolate biosynthesis pathway [ 42 ], was demonstrated to be distributed only in the nine Brassicaceae species and M. esculenta among the 16 plant species investigated in this study. Conversely, five subfamilies (S35, S43, S45, S47, and S48) were absent from the nine investigated Brassicaceae species.…”

“…), four asterid-specific subfamilies (S39, S40, S43, and S80), and nine subfamilies (S29–36 and S69) specific to one of the 35 investigated species were identified [ 43 ]. Similarly, many subfamilies/clusters/clades specific to different lineages and species were found in numerous studies [ 42 , 43 , 44 , 45 , 46 , 47 ]. For example, subfamily S29 was proven to harbor only potato genes [ 42 ], seven subfamilies (e.g., S1, S5, S11, etc.)…”

“…As shown in Figure 1 A, some of the 429 Bn2R-MYB s were clustered into five new subfamilies (S74–S78) alongside several A. thaliana ‘ orphan’ genes ( Figure S1 ), indicating the expansion trend of the Bn2R-MYB family, and perhaps functional diversification of its members. Genome-wide analyses of the 2R-MYB gene family have been carried out in many plant species such as A. thaliana [ 22 ], rice [ 23 ], Populus trichocarpa [ 40 ], Ananas comosus [ 41 ], and potato ( Solanum tuberosum ) [ 42 ]. The classification of this gene family in these studies commonly refers to the results obtained from A. thaliana [ 22 ], which includes 25 main subfamilies.…”

Changes in Ecophysiology, Osmolytes, and Secondary Metabolites of the Medicinal Plants of Mentha piperita and Catharanthus roseus Subjected to Drought and Heat Stress

“…One of the biggest issues concerning the impact of climate change on human being and natural ecosystems is the reaction of the Earth's hydrologic cycle to global warming [1,2]. As sessile species,…”

Abscisic Acid and Jasmonate Metabolisms Are Jointly Regulated During Senescence in Roots and Leaves of Populus trichocarpa

“…MYB TFs are a large family of proteins that are a crucial element in regulatory networks controlling plant development, metabolism, and responses to biotic and abiotic stresses [89]. The up-regulation of several MYB proteins was documented in senescing leaves of Arabidopsis [90] and Solanum [91]. MYB TFs are also involved in the jasmonate signaling cascade by interacting with JAZ proteins [76,92], which are known as repressors of JA-responsive genes.…”