System peaks in capillary zone electrophoresis What are they and where are they coming from?

Beckers, JL Jozef; Everaerts, FM Frans

doi:10.1016/s0021-9673(97)00612-2

Cited by 34 publications

(36 citation statements)

References 14 publications

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“…The existence of the discontinuity in the diverse relationships of panels A±H of a SystChart indicates the presence of such a system peak and its corresponding mobility. As already shown, the measured mobilities of such SPs agree with calculated mobilities [16]. For SystCharts with two or more coions the existence of SPs is indicated by the discontinuities in the SystCharts.…”

Section: Remarkable Phenomena In Systcharts For Bges With Two Coionssupporting

confidence: 84%

“…Theoretically, the existence of SPs in a specific BGE can be deduced from the discontinuities in SystCharts. The SP mobilities can be calculated using a mathematical model describing the moving boundaries in CZE; these are the zones with a composition deviating from that of the BGE concerning concentrations of the ionic species of the BGE and/or pH [16]. Experimentally, SPs are measured by applying several BGEs at low pH.…”

Section: Discussionmentioning

confidence: 99%

“…In SystCharts calculated for BGEs consisting of two coions [13,16] the relationships between the parameters versus the mobilities of the analytes in several of the panels (A±H) exhibit discontinuities. histidine in the sample zone strongly increases and that of potassium strongly decreases.…”

Section: Remarkable Phenomena In Systcharts For Bges With Two Coionsmentioning

confidence: 99%

See 2 more Smart Citations

Peaks in capillary zone electrophoresis: Fact or fiction

Beckers

Boček

1999

Electrophoresis

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Section: Remarkable Phenomena In Systcharts For Bges With Two Coionssupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Peaks in capillary zone electrophoresis: Fact or fiction

Beckers

Boček

1999

Electrophoresis

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“…s j (t) in Eq. (1)) (Beckers and Everaerts 1997;Gaš et al 2007;Gebauer and Boček 1997;Macka et al 1997;Sellmeyer and Poppe 2002). Fig.…”

Section: Peak Modelsmentioning

confidence: 96%

Signal Processing Methods for Capillary Electrophoresis

Stewart¹,

Gideoni²,

Zhu³

2011

Systems and Computational Biology - Bioinformatics and Computational Modeling

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“…These zones migrate with a specific mobility through the electrophoretic system and are often called "eigen zones", "system zones" or "system peaks" [27][28][29][30][31][32]. SZs play a very important role in the electrophoretic process, and, their behavior is frequently decisive for the failure or success of the analysis.…”

Section: System Zones In Czementioning

confidence: 99%

The preparation of background electrolytes in capillary zone electrophoresis: Golden rules and pitfalls

Beckers

Boček

2003

Electrophoresis

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In this article the methodology of the design of suitable background electrolytes (BGEs) in capillary zone electrophoresis (CZE) is described. The principal aspects of the role of a BGE in CZE are discussed with respect to an appropiate migration behavior of analytes, including the transport of the electric current, the buffering of pH, the Joule heat, the electro-endosmotic flow (EOF) and the principal migration and detection modes. The impact of the composition of the BGE upon migration and detection is discussed. It is shown that the total concentration of the BGE is a principal factor and the adjustment of migrating analyte zones according to the Kohlrausch regulating function (KRF) is the principal effect in most of the sample stacking techniques. The number of co-ions and their properties are of key importance for peak shapes of the analyte peaks and for the existence of system zones. The detection of UV-transparent analytes may advanteously be done in the indirect UV mode, by using UV-absorbing co-ions, however, both peaks and dips may be expected in the UV trace in case of multiple co-ionic BGEs. Properties of BGEs can be predicted applying mathematical models and it is shown that with SystCharts, predictions can be given concerning the existence of system zones, detection modes and the peak shapes of analytes for a given BGE. Practical examples of methodological considerations are given in the design of suitable BGEs for four principal combinations of migration and detection modes. The properties of the BGEs selected are exemplified with experimental results. Golden rules are summarized for the preparation of suitable BGEs in CZE.

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System peaks in capillary zone electrophoresis What are they and where are they coming from?

Cited by 34 publications

References 14 publications

Peaks in capillary zone electrophoresis: Fact or fiction

Peaks in capillary zone electrophoresis: Fact or fiction

Signal Processing Methods for Capillary Electrophoresis

The preparation of background electrolytes in capillary zone electrophoresis: Golden rules and pitfalls

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