System for delivering microwave ablation to subcutaneous tumors in small-animals under high-field MRI thermometry guidance

Šebek, Jan; Shrestha, Tej B.; Basel, Matthew T.; Chamani, Faraz; Zeinali, Nooshin; Mali, Ivina; Payne, Macy; Timmerman, Sarah A.; Faridi, Pegah; Pyle, Marla; O’Halloran, Martin; Dennedy, Michael Conall; Bossmann, Stefan H.; Prakash, Punit

doi:10.1080/02656736.2022.2061727

Cited by 4 publications

(6 citation statements)

References 40 publications

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“…and Sebek et al. ( 21 , 22 ). The antenna was secured with tape and a plastic cradle along the skin to allow for repositioning of the antenna while the mouse remained in the scanner.…”

Section: Methodsmentioning

confidence: 89%

T1-mapping characterization of two tumor types

Payne,

Mali,

Shrestha

et al. 2024

Biophysical Reports

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“…and Sebek et al. ( 21 , 22 ). The antenna was secured with tape and a plastic cradle along the skin to allow for repositioning of the antenna while the mouse remained in the scanner.…”

Section: Methodsmentioning

confidence: 89%

T1-mapping characterization of two tumor types

Payne,

Mali,

Shrestha

et al. 2024

Biophysical Reports

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“…Mice in the treatment group were anesthetized with isoflurane and the tumor was heated to temperatures of 55 °C or higher with a custom 2.45 GHz microwave antenna using MRI thermometry feedback as previously described. 35 Similar to the treatment mice, control group mice were anesthetized with isoflurane and imaged by MRI, but did not receive microwave ablation. Twenty-four hours after treatment or mock-treatment, mice were euthanized using a CO 2 chamber and tumors, lung, liver, kidney, spleen, small intestine, and brain were collected.…”

Section: Methodsmentioning

confidence: 99%

Section: Treating Tumors With Microwave Ablationmentioning

confidence: 99%

“…34 In previous work, our group has developed and tested an in vivo porcine model that utilized directed microwave radiation for potential thermal treatment of aldosterone-producing adenomas. 35 This work, however, did not include a functioning tumor target, demonstrating the need for a better characterized model of neoplastic PA that is useful for testing thermal therapies.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Biochemical Mouse Model of Primary Aldosteronism for Thermal Therapies

Timmerman,

Mullen,

Taylor

et al. 2024

Preprint

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Introduction Aldosterone-producing adenoma (APA) is the most common cause of endocrine-related hypertension but surgery is not always feasible. Current medical interventions are associated with significant side effects and poor patient compliance. New APA animal models that replicate basic characteristics of APA and give physical and biochemical feedback are needed to test new non-surgical treatment methods, such as image-guided thermal ablation, Methods A model of APA was developed in nude mice using HAC15 cells, a human adrenal carcinoma cell line. Tumor growth, aldosterone production, and sensitivity to angiotensin II were characterized in the model. The utility of the model was validated via treatment with microwave ablation and characterization of the resulting physical and biochemical changes in the tumor. Results The APA model showed rapid and relatively homogeneous growth. The tumors produced aldosterone and steroid precursors in response to angiotensin II challenge, and plasma aldosterone levels were significantly higher in tumor bearing mice two hours after challenge verses non-tumor bearing mice. The model was useful for testing microwave ablation therapy, reducing aldosterone production by 80% in treated mice. Conclusion The HAC15 model is a useful tumor model to study and develop localized treatment methods for APA.

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“…During thermal ablation, a characteristic heat map of radially decreasing temperature arises from the position of the thermal ablation applicator probe tip, outwards from the so-called ablation zone (T > 50°C ( 14 , 15 )) through to the transitional zone (42 < T < 45°C) between ablated and nonablated tissue ( 16 ) ( Fig. 1 ).…”

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confidence: 99%

Sublethal Hyperthermia Transiently Disrupts Cortisol Steroidogenesis in Adrenocortical Cells

et al. 2023

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Primary Aldosteronism is the most common cause of secondary hypertension. First-line treatment; adrenalectomy resects adrenal nodules and adjacent normal tissue, limiting suitability to those who present with unilateral disease. Use of thermal ablation represents an emerging approach as a possible minimally invasive therapy for unilateral and bilateral disease, to target and disrupt hypersecreting aldosterone producing adenomas, while preserving adjacent normal adrenal cortex. To determine the extent of damage to adrenal cells upon exposure to hyperthermia; the steroidogenic adrenocortical cell lines, H295R and HAC15 were treated with hyperthermia at temperatures between 37-50 °C with the effects of hyperthermia on steroidogenesis evaluated following stimulation with forskolin (FSK) and angiotensin II (ANGII). Cell death, protein/mRNA expression of steroidogenic enzymes and damage markers (HSP70/90), and steroid secretion were analysed immediately and 7-days post-treatment. Following treatment with hyperthermia, 42 °C & 45 °C, did not induce cell death and were deemed sublethal doses while ≥50 °C caused excess cell death in adrenal cells. Sublethal hyperthermia (45 °C) caused a significant reduction in cortisol secretion immediately following treatment while differentially affecting the expression of various steroidogenic enzymes, although recovery of steroidogenesis was evident 7-days post-treatment. As such, sublethal hyperthermia, which occurs in the transitional zone during thermal ablation induces a short-lived, un-sustained inhibition of cortisol steroidogenesis in adrenocortical cells in vitro.

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System for delivering microwave ablation to subcutaneous tumors in small-animals under high-field MRI thermometry guidance

Cited by 4 publications

References 40 publications

T1-mapping characterization of two tumor types

T1-mapping characterization of two tumor types

Characterization of a Biochemical Mouse Model of Primary Aldosteronism for Thermal Therapies

Sublethal Hyperthermia Transiently Disrupts Cortisol Steroidogenesis in Adrenocortical Cells

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