Syrian hamster infected with <i>Leishmania infantum</i>: A new experimental model for inflammatory myopathies

Paciello, Orlando; Wójcik, Sławomir; Gradoni, Luigi; Oliva, Gætano; Trapani, Francesca; Iovane, Valentina; Politano, Luisa; Papparella, Serenella

doi:10.1002/mus.21502

Cited by 21 publications

(17 citation statements)

References 23 publications

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“…Syrian hamsters also exhibit severe clinical signs and symptoms that are similar to those observed in naturally infected dogs and humans [23,24,36]. However, the absence of iNOS expression [10,28,31] and the suppression of lymphoproliferative responses [64,65] observed, lead us to argue that the hamster is a more suitable model for pathological studies of VL than for the evaluation of vaccine candidates.…”

Section: Remarks and Discussionmentioning

confidence: 99%

“…In this context, BALB/c mice provide a better model of self-healing or subclinical infection than of disseminated visceral disease [10]. Paciello et al [36] reported that susceptible mouse strains do not reproduce progressive disease as observed in human active VL. Furthermore, the intensity of pathological changes in the visceral organs of BALB/c mice can vary depending on the initial inoculum size, as described above.…”

Section: Remarks and Discussionmentioning

confidence: 99%

“…The visceral infection in hamsters also induces pathological alterations in hepatocytes, mainly in the endomembrane system and the peroxisomal compartment, leading to a disturbance of liver metabolism [35]. In a recent study [36], hamsters infected with L. infantum were shown to develop analogous inflammatory myopathies to those observed in naturally infected dogs [37]. Taken together, all these factors probably contributed to the immune response disorders that resulted in the death of the animals [22,33].…”

Section: Syrian Hamster Model Of Vl: Suitability Of This Experimenmentioning

confidence: 99%

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Mechanisms of resistance and susceptibility to experimental visceral leishmaniosis: BALB/c mouse versus syrian hamster model

Nieto¹,

Domínguez‐Bernal

Orden

et al. 2011

Vet Res

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Several animal models have been established to study visceral leishmaniosis (VL), a worldwide vector-borne disease affecting humans and domestic animals that constitutes a serious public health problem. BALB/c mice and Syrian hamsters are the most widely used experimental models. In this paper, we summarize the advantages and disadvantages of these two experimental models and discuss the results obtained using these models in different studies of VL. Studies using the BALB/c mouse model have underscored differences between the liver and spleen in the course of VL, indicating that pathological evaluation of the visceral organs is essential for understanding the immune mechanisms induced by Leishmania infantum infection. The main goal of this review is to collate the relevant literature on Leishmania pathogenesis into a sequence of events, providing a schematic view of the main components of adaptive and innate immunity in the liver and spleen after experimental infection with L. infantum or L. donovani. This review also presents several viewpoints and reflections about some controversial aspects of Leishmania research, including the choice of experimental model, route of administration, inoculum size and the relevance of pathology (intimately linked to parasite persistence): a thorough understanding of which is essential for future VL research and the successful development of efficient control strategies for Leishmania spp.

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Section: Remarks and Discussionmentioning

confidence: 99%

Section: Remarks and Discussionmentioning

confidence: 99%

Section: Syrian Hamster Model Of Vl: Suitability Of This Experimenmentioning

confidence: 99%

See 1 more Smart Citation

Mechanisms of resistance and susceptibility to experimental visceral leishmaniosis: BALB/c mouse versus syrian hamster model

Nieto¹,

Domínguez‐Bernal

Orden

et al. 2011

Vet Res

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“…Those for which such an infiltration has been demonstrated are the model of crush-injury described above [7] and models designed to help understand the mechanisms of inflammatory myopathies that occur in man. In these latter cases, myositis is induced by various infectious agents [53, 54], immunization with muscle components, for example, myosin [55, 56], and intraperitoneal injections with lipopolysaccharide [57]. The TNF system has not been examined in any of these myositis models replicating the inflammatory myopathies seen in man.…”

Section: Discussionmentioning

confidence: 99%

TNF-Alpha in the Locomotor System beyond Joints: High Degree of Involvement in Myositis in a Rabbit Model

Forsgren

Renström

Purdam³

et al. 2012

International Journal of Rheumatology

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The importance of TNF-alpha in arthritis is well documented. It may be that TNF-alpha is also markedly involved in muscle inflammation (myositis). An animal model where this can be investigated is needed. A newly developed rabbit myositis model involving pronounced muscle overuse and local injections of substances having proinflammatory effects was therefore used in the present study. The aim was to investigate the patterns of TNF-alpha expression in the developing myositis and to evaluate the usefulness of this myositis model for further TNF-alpha research. Human rheumatoid arthritis (RA) synovial tissue was examined as a reference. TNF-alpha immunoexpression and TNF-alpha mRNA, visualized via in situ hybridization, were detected in cells in the inflammatory infiltrates of the affected muscle (soleus muscle). Coexistence of TNF-alpha and CD68 immunoreactions was noted, suggesting that the TNF-alpha reactive cells are macrophages. Expression of TNF-alpha mRNA was also noted in muscle fibers and blood vessel walls in areas with inflammation. These findings demonstrate that TNF-alpha is highly involved in the myositis process. The model can be used in further studies evaluating the importance of TNF-alpha in developing myositis.

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“…Primary antibodies used in this study included rabbit polyclonal Beclin-1 (BECN 1 H300: sc-11,427, Santa Cruz Biotechnology, Dallas, Texas, US), rabbit polyclonal LC3 (ab51520, Abcam, Cambridge, UK), mouse monoclonal APP (6E10) (SIG-39320, BioLegend, San Diego, California, US). The method was previously described [29]. Briefly, the primary antibody diluted in Phosphate Buffer Saline (PBS) was applied overnight at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Amyloid precursor protein, lipofuscin accumulation and expression of autophagy markers in aged bovine brain

et al. 2017

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BackgroundAutophagy is a highly regulated process involving the bulk degradation of cytoplasmic macromolecules and organelles in mammalian cells via the lysosomal system. Dysregulation of autophagy is implicated in the pathogenesis of many neurodegenerative diseases and integrity of the autophagosomal - lysosomal network appears to be critical in the progression of aging. Our aim was to survey the expression of autophagy markers and Amyloid precursor protein (APP) in aged bovine brains. For our study, we collected samples from the brain of old (aged 11–20 years) and young (aged 1–5 years) Podolic dairy cows. Formalin-fixed and paraffin embedded sections were stained with routine and special staining techniques. Primary antibodies for APP and autophagy markers such as Beclin-1 and LC3 were used to perform immunofluorescence and Western blot analysis.ResultsHistologically, the most consistent morphological finding was the age-related accumulation of intraneuronal lipofuscin. Furthermore, in aged bovine brains, immunofluorescence detected a strongly positive immunoreaction to APP and LC3. Beclin-1 immunoreaction was weak or absent. In young controls, the immunoreaction for Beclin-1 and LC3 was mild while the immunoreaction for APP was absent. Western blot analysis confirmed an increased APP expression and LC3-II/LC3-I ratio and a decreased expression of Beclin-1 in aged cows.ConclusionsThese data suggest that, in aged bovine, autophagy is significantly impaired if compared to young animals and they confirm that intraneuronal APP deposition increases with age.

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Syrian hamster infected with Leishmania infantum: A new experimental model for inflammatory myopathies

Cited by 21 publications

References 23 publications

Mechanisms of resistance and susceptibility to experimental visceral leishmaniosis: BALB/c mouse versus syrian hamster model

Mechanisms of resistance and susceptibility to experimental visceral leishmaniosis: BALB/c mouse versus syrian hamster model

TNF-Alpha in the Locomotor System beyond Joints: High Degree of Involvement in Myositis in a Rabbit Model

Amyloid precursor protein, lipofuscin accumulation and expression of autophagy markers in aged bovine brain

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