SYRCLE’s risk of bias tool for animal studies

Hooijmans, Carlijn R.; Rovers, Maroeska M.; Vries, Rob B. M. de; Leenaars, Marlies; Ritskes‐Hoitinga, Merel; Langendam, Miranda

doi:10.1186/1471-2288-14-43

Cited by 2,438 publications

(2,226 citation statements)

References 29 publications

(28 reference statements)

Supporting

Mentioning

2,041

Contrasting

Unclassified

Order By: Relevance

“…If there is any addition or exclusion, it should be noted with suitable reasons. On the other hand, we also agree with Hooijmans et al [77] that the registration of all animal studies should be more common and their protocols should be published in accessible databases with the hope of improving the standards of these studies. Thereby, further studies in humans would be more accurate.…”

Section: Discussionsupporting

confidence: 89%

“…The methodological quality of included animal studies was assessed (▶ Table 4) by using criteria from SYRCLEʼs tool [77]. This tool contains 10 items: sequence generation (selection bias), baseline characteristics (selection bias), allocation concealment (selection bias), random housing (performance bias), blinding (performance bias), random outcome assessment (detection bias), blinding (detection bias), incomplete outcome data (attrition bias), selective outcome reporting (reporting bias), and other sources of bias (other bias).…”

Section: Methodsological Quality Of Studiesmentioning

confidence: 99%

See 1 more Smart Citation

Ginsenoside Rh1: A Systematic Review of Its Pharmacological Properties

et al. 2018

View full text Add to dashboard Cite

Supporting information available online at http://www.thieme-connect.de/products ABSTR AC TGinsenoside Rh1 is one of major bioactive compounds extracted from red ginseng, which has been increasingly used for enhancing cognition and physical health worldwide. The objective of this study was to review the pharmacological effects of ginsenoside Rh1 in a systematic manner. We performed searches on eight electronic databases including MEDLINE (Pubmed), Scopus, Google Scholar, POPLINE, Global Health Library, Virtual Health Library, the System for Information on Grey Literature in Europe, and the New York Academy of Medicine Grey Literature Report to select the original research publications reporting the biological and pharmacological effects of ginsenoside Rh1 from in vitro and in vivo studies regardless of publication language and study design. Upon applying the inclusion and exclusion criteria, we included a total of 57 studies for our systemic review. Ginsenoside Rh1 exhibited the potent characteristics of anti-inflammatory, antioxidant, immunomodulatory effects, and positive effects on the nervous system. The cytotoxic effects of ginsenoside Rh1 were dependent on different types of cell lines. Other pharmacological effects including estrogenic, enzymatic, anti-microorganism activities, and cardiovascular effects have been mentioned, but the results were considerably diverged. A higher quality of evidence on clinical trial studies is highly recommended to confirm the consistent efficacy of ginsenoside Rh1.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Methodsological Quality Of Studiesmentioning

confidence: 99%

Ginsenoside Rh1: A Systematic Review of Its Pharmacological Properties

et al. 2018

View full text Add to dashboard Cite

show abstract

“…To access the risk of bias of RCTs, the Cochrane Collaboration has developed the Cochrane RoB Tool (41). Systematic reviews of experimental animal studies are not yet common practice, and just recently the SYstematic Review Centre for Laboratory animal Experimentation has presented a SYRCLE's RoB Tool for animal studies (42). The SYRCLE's RoB Tool is based on the Cochrane RoB Tool and has been adjusted for the aspects of bias known to play a role in animal intervention studies.…”

Section: Methodsmentioning

confidence: 99%

The impact of vitamin D on fetal and neonatal lung maturation. A systematic review

Lykkedegn

Sørensen

Beck-Nielsen

et al. 2015

American Journal of Physiology-Lung Cellular and Molecular Phys

117

104

View full text Add to dashboard Cite

-Respiratory distress syndrome (RDS) and bronchopulmonary dysplasia (BPD) are major complications to preterm birth. Hypovitaminosis D is prevalent in pregnancy. We systematically reviewed the evidence of the impact of vitamin D on lung development, surfactant synthesis, RDS, and BPD searching PubMed, Embase, and Cochrane databases with the terms vitamin D AND (surfactant OR lung maturation OR lung development OR respiratory distress syndrome OR fetal lung OR prematurity OR bronchopulmonary dysplasia). Three human studies, ten animal studies, two laboratory studies, and one combined animal and laboratory study were included. Human evidence was sparse, allowing no conclusions. BPD was not associated with vitamin D receptor polymorphism in a fully adjusted analysis. Animal and laboratory studies showed substantial positive effects of vitamin D on the alveolar type II cell, fibroblast proliferation, surfactant synthesis, and alveolarization. These data support the hypothesis of hypovitaminosis D as a frequent, modifiable risk factor of RDS and BPD, which should be tested in randomized controlled trials on pregnant women, those with threatening preterm delivery, or in the preterm neonates. Future experimental and human studies should aim to identify optimal time windows, vitamin D doses, and cut-off levels for 25-hydroxyvitamin D in interventions against RDS, BPD, and later adverse respiratory outcomes. vitamin D; fetus; lung; surfactant; preterm; neonate THE IMPORTANCE OF VITAMIN D in nonskeletal fetal and childhood health has become increasingly recognized in the last decades (19,65). The impact of vitamin D on early lung development and maturation and lung diseases of early life is an emerging field of research. Hypovitaminosis D during pregnancy is associated with reduced placental development and weight, leading to a presumed risk of preterm birth (24,25), which per se may lead to respiratory distress syndrome (RDS) and bronchopulmonary dysplasia (BPD). Moreover, vitamin D is suggested to play a role in the embryogenesis and in cellular growth and differentiation, including lung development and regulation of lung maturation in the fetus (23,29,38,70,71,108), whereby hypovitaminosis D could be hypothesized to aggravate premature neonatal lung diseases.Vitamin D is involved in the development of the innate and adaptive immune system, and, because of its unique capacity to bind to the vitamin D receptor (VDR) and thereby to serve as a transcriptional factor, vitamin D can regulate gene expression and exert widespread immunomodulatory effects including proliferation, differentiation, and complex regulation (19, 56, 58 -61, 64, 65, 68, 69, 105). Evidence suggests that vitamin D may stimulate regulatory T cell development and T cell function to suppress inappropriate Th1 and Th2 responses to environmental exposure (i.e., allergens, infection load), leading to a more balanced immune response inhibiting autoimmune diseases (with Th1 dominance) and allergic diseases (Th2 dominance) (55). Vitamin D could therefore be inv...

show abstract

“…Among the papers found from the literature survey, the published papers discussing the improvement of standards of reporting in animal studies, 10-13 a recently introduced RoB tool 14 and CAMARADES checklist 15 were selected to develop the initial questionnaire. By excluding the therapeutic variables from the proposed items in the aforementioned papers, a 47-item checklist was developed (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Potential variables affecting the quality of animal studies regarding pathophysiology of traumatic spinal cord injuries

et al. 2015

View full text Add to dashboard Cite

Study design: This is a Delphi study. Objectives: Defining variables that potentially influence the outcomes of an animal study regarding pathophysiology of traumatic spinal cord injury (TSCI). Setting: This study was conducted in Iran. Methods: A modified two-round Delphi study was conducted. As the first round, an initial questionnaire was developed on the basis of literature and a series of focus group discussions. In the second round, the participants were asked to score the items through a 10-point scale. Consensus was achieved through the following criteria: (1) the median of scores has to be at 7.5 or higher, and (2) at least 70% of participants need to rate 7 or higher. Also, the inter-rater reliability analysis was performed to determine consistency among raters using the Kappa coefficient and Cronbach's alpha. Results: Twenty-one experts participated in our study. From the first round of the study, a 47-item checklist was developed. By considering the aforementioned criteria for consensus building on extremely important factors, we reached a 15-item checklist including species, strain, method and level of injury, control group, genetic background, severity of injury, attrition, use of appropriate test, blindness, method of allocation to treatments, regulation and ethics, age/weight, bladder expression, number of animals/group and statistics. The inter-rater reliability for the raters was found to be Kappa = 0.82 (Po0.001). A Cronbach's alpha of 0.9 for all the questions indicated high internal consistency. Conclusion: This study introduces a checklist of variables that potentially influence the outcomes of animal studies regarding TSCI pathophysiology and describe its validity and reliability. Spinal Cord (2016) 54, 579-583; doi:10.1038/sc.2015.215; published online 22 December 2015 INTRODUCTIONTraumatic spinal cord injury (TSCI) is a devastating injury to the patient, family and society, as a TSCI results in multi-system complications, impaired health-related quality of life and poor prognosis for neurologic recovery. 1-3 The incidence of TSCI ranges from 3.6 to 195.4 patients per million around the world 4 and 25.5 per million per year in developing countries. 5 A prerequisite to the development of effective therapies for TSCI is a detailed understanding of the pathophysiological events occurring after injury. The pathophysiology of TSCI can be well divided into two phases, a primary and a secondary injury. The primary injury involves the initial mechanical damage, which is followed by a delayed onset of a secondary phase comprising a cascade of molecular and cellular events that inhibits the regeneration and also extends the injured site. As a result of the many technical limitations associated with the study of TSCI pathophysiology in humans, almost all of our detailed understanding of TSCI pathophysiology is based on animal studies. Translation of the obtained outcomes to the clinic could be achieved through well-designed animal studies and consideration of the variables, which are decisive in det...

show abstract

SYRCLE’s risk of bias tool for animal studies

Cited by 2,438 publications

References 29 publications

Ginsenoside Rh1: A Systematic Review of Its Pharmacological Properties

Ginsenoside Rh1: A Systematic Review of Its Pharmacological Properties

The impact of vitamin D on fetal and neonatal lung maturation. A systematic review

Potential variables affecting the quality of animal studies regarding pathophysiology of traumatic spinal cord injuries

Contact Info

Product

Resources

About